Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	89,128	T→A	5.7%	V134V (GTT→GTA)	lysS →	lysyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	89,128	0	T	A	5.7%	262.3 / 2.5	81	V134V (GTT→GTA)	lysS	lysyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base A (3/2);  total (41/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAAATCCAAATCTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCAAACATACG  >  BS168_reference_genome/88995‑89263                                                                                                                                      |                                                                                                                                        gCCAAATCCAAATCTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAg                                                                                                                                     >  1:192468/1‑138 (MQ=255)    aaatccaaatcTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAAc                                                                                                                                 <  3:309091/139‑1 (MQ=255)          aaatcTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCtt                                                                                                                           >  5:49994/1‑139 (MQ=255)           aatcTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCttt                                                                                                                          >  5:229833/1‑139 (MQ=255)                 cGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTg                                                                                                                    >  4:27108/1‑139 (MQ=255)                 cGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTAAAAGCAACTTCCTTTGAATTg                                                                                                                    >  4:250927/1‑139 (MQ=255)                           gACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAg                                                                                                          >  8:110398/1‑139 (MQ=255)                              aGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAgcgc                                                                                                       >  7:39663/1‑139 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCt                                                                                                                  <  5:52058/126‑1 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCt                                                                                                                  >  6:52058/1‑126 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     >  7:75918/1‑139 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     >  4:294416/1‑139 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     <  6:49994/139‑1 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     >  3:16847/1‑139 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     >  2:106193/1‑139 (MQ=255)                                tgtCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCtt                                                                                                     >  7:61103/1‑139 (MQ=255)                                tgtCGGTGACGACCAATAAGAAATCTTCAAGTCTTCTAACCTCCGTGATCTTATCGGCGTAACCCGAAAACTCTTCAAAACAAAAGTAGCCGAATTGTCTGTTAAATCAACTACCTTTGAATTTATGACAAAAGCGCtt                                                                                                     >  2:102754/1‑139 (MQ=255)                                  tCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCg                                                                                                   >  2:189202/1‑139 (MQ=255)                                    ggTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCgtcgtc                                                                                                 <  3:27108/139‑1 (MQ=255)                                     gTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTcc                                                                                                >  3:117473/1‑139 (MQ=255)                                     gTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGAGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTTAAGCAACTTCCTTTTAATTTCTGACAAAAGCGCTTCGTcc                                                                                                >  4:162165/1‑139 (MQ=255)                                          gacCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTc                                                                                           >  7:43202/1‑139 (MQ=255)                                          gacCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTc                                                                                           >  8:229957/1‑139 (MQ=255)                                            ccAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCt                                                                                         >  2:74308/1‑139 (MQ=255)                                            ccAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCt                                                                                         >  8:190054/1‑139 (MQ=255)                                               atatGAAATCTTCAAGTCTTCTGAGCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGAc                                                                                      <  5:149748/139‑1 (MQ=255)                                               atatGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGAc                                                                                      <  8:61103/139‑1 (MQ=255)                                                     aaTCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGAc                                                                                      >  3:204354/1‑133 (MQ=255)                                                     aaTCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGAc                                                                                      <  4:204354/133‑1 (MQ=255)                                                      aTCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATAc                                                                                >  5:220192/1‑138 (MQ=255)                                                          tCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATgg                                                                           >  5:214470/1‑139 (MQ=255)                                                          tCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTAAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATgg                                                                           >  5:13946/1‑139 (MQ=255)                                                          tCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAAATGTCTGTAAAAGCAACTTCCATTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCAtcg                                                                           >  5:9715/1‑137 (MQ=255)                                                           cacggcttctGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTAAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCa                                                                              <  6:9715/130‑1 (MQ=255)                                                           cAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCa                                                                              <  3:162165/135‑1 (MQ=255)                                                           cAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTACTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGt                                                                          >  4:37490/1‑139 (MQ=255)                                                           cAAGTCTTCTGACCTCGGTGATCTTATCGGCGGAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTAAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCa                                                                              <  6:13946/135‑1 (MQ=255)                                                                 ttctGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGAGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTaaaa                                                                    >  4:217589/1‑139 (MQ=255)                                                                   ctGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTaaa                                                                     <  7:123045/136‑1 (MQ=255)                                                                      aCCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACg                                                                <  2:192468/138‑1 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           >  5:64974/1‑138 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           <  1:106193/138‑1 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           >  5:304787/1‑138 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           <  1:74308/138‑1 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           >  4:17789/1‑138 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           <  8:39663/138‑1 (MQ=255)                                                                           ggTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           <  3:294416/138‑1 (MQ=255)                                                                           ggTGATCTTAACGGCGTAACCGTAAAATTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTTAAAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGag                                                           <  2:199846/138‑1 (MQ=255)                                                                            gTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGagca                                                         <  6:304787/139‑1 (MQ=255)                                                                               ttcttctCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGTCAAATACCATGGTTTAAAAGACGTTGAGCAgcg                                                      <  5:206884/138‑1 (MQ=255)                                                                                    aTCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCt                                                    <  7:110398/136‑1 (MQ=255)                                                                                    aTCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCt                                                    <  6:229833/136‑1 (MQ=255)                                                                                    aTCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCGTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAgcg                                                      <  5:203006/134‑1 (MQ=255)                                                                                     tCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTc                                                                                           <  4:32007/96‑1 (MQ=255)                                                                                     tCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTc                                                                                           >  3:32007/1‑96 (MQ=255)                                                                                        gCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTc                                                                                                 <  4:49698/87‑1 (MQ=255)                                                                                        gCGTAACCGGAAAAGACTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTc                                                                                                 >  3:49698/1‑87 (MQ=255)                                                                                         cGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACg                                                                <  5:205205/119‑1 (MQ=255)                                                                                         cGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACg                                                                >  6:205205/1‑119 (MQ=255)                                                                                         cGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAg                                            >  3:308841/1‑139 (MQ=255)                                                                                         cGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAg                                            <  6:214470/139‑1 (MQ=255)                                                                                          gTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgc                                           <  5:16818/139‑1 (MQ=255)                                                                                           tAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          >  8:40770/1‑139 (MQ=255)                                                                                           tAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          <  7:40770/139‑1 (MQ=255)                                                                                           tAAACGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCATCAgcg                                          <  8:180853/139‑1 (MQ=255)                                                                                                      gTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATc                               <  1:189202/139‑1 (MQ=255)                                                                                                       tCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCt                              <  6:214093/139‑1 (MQ=255)                                                                                                         ttCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          <  6:110632/125‑1 (MQ=255)                                                                                                         ttCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          >  5:110632/1‑125 (MQ=255)                                                                                                            aaaaCAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCg                         <  4:16847/139‑1 (MQ=255)                                                                                                            aaaaCAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCg                         <  3:250927/139‑1 (MQ=255)                                                                                                                 aaaTGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          <  7:289327/117‑1 (MQ=255)                                                                                                                 aaaTGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAgcg                                          >  8:289327/1‑117 (MQ=255)                                                                                                                   aTGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCa                                                                              >  7:192211/1‑79 (MQ=255)                                                                                                                   aTGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCa                                                                              <  8:192211/79‑1 (MQ=255)                                                                                                                   aTGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAAcc                   >  7:27842/1‑138 (MQ=255)                                                                                                                      tAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGa               <  3:217589/139‑1 (MQ=255)                                                                                                                           tacttGTCTGTTAAAGCATCTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCa          <  6:312871/136‑1 (MQ=255)                                                                                                                           gAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCa          <  7:190054/139‑1 (MQ=255)                                                                                                                                 tCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCAAACAt     <  8:75918/138‑1 (MQ=255)                                                                                                                                   tGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCAAACATACg  >  2:36366/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        GCCAAATCCAAATCTACGTAAGAAAAGACAGTGTCGGTGACGACCAATATGAAATCTTCAAGTCTTCTGACCTCGGTGATCTTATCGGCGTAACCGGAAAAGTCTTCAAAACAAATGTAGGCGAATTGTCTGTTAAAGCAACTTCCTTTGAATTGCTGACAAAAGCGCTTCGTCCGCTTCCTGACAAATACCATGGTTTAAAAGACGTTGAGCAGCGCTACCGTCAGCGCTATCTGGATCTTATCGTAAACCCAGACAGCAAACATACG  >  BS168_reference_genome/88995‑89263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑