| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,345,064 | C→T | 6.1% | G117G (GGG→GGA) | nth ← | endonuclease III, apurinic apyrimidic DNA lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,345,064 | 0 | C | T | 6.1% | 211.7 / 2.7 | 65 | G117G (GGG→GGA) | nth | endonuclease III, apurinic apyrimidic DNA lyase |
| Reads supporting (aligned to +/- strand): ref base C (35/26); new base T (2/2); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01 | |||||||||||
TTTTCAACTTCCAGAACCGAGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTAATATC > BS168_reference_genome/2344927‑2345189 | ttttCAACTTCCAGAACCGAGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGAccc > 3:88861/1‑139 (MQ=255) aCTTCCAGAACAGAGTCCTTCCACCGGCAAATGCCCATTCGATTGCTGACTCTCTCCTCATGTGTATCTACGGGTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCa < 8:128509/139‑1 (MQ=255) atccccaaCCCAGCCCATCCCCCCGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGCGTATCTACGGCTAAGGCCGGCACGCCAAACCCAACGGAAACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGt < 6:296765/133‑1 (MQ=255) ccAGAACCGAGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGttt > 5:296765/1‑139 (MQ=255) gAACCGAGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGAc < 4:106241/139‑1 (MQ=255) aGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCt > 6:304258/1‑138 (MQ=255) ccACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATcgc < 8:276938/137‑1 (MQ=255) ccACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATcgc < 2:244015/137‑1 (MQ=255) ccACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCgg > 7:276938/1‑139 (MQ=255) tcCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa > 1:50776/1‑139 (MQ=255) cacaTGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCgg < 7:290260/111‑1 (MQ=255) cacaTGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCgg > 8:290260/1‑111 (MQ=255) cacaTGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa < 4:161872/137‑1 (MQ=255) ggTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTATCCCGACCCCTGGCAGTATGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCAtatt > 2:113204/1‑138 (MQ=255) tCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTc < 7:126594/138‑1 (MQ=255) cTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCtttt > 2:251362/1‑138 (MQ=255) aTGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAAACTTCAATAATCATTTTAATAAGCATTTTaa > 2:158240/1‑139 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATCCCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGaa > 2:20667/1‑138 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCgg > 4:36517/1‑91 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCgg < 3:36517/91‑1 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAat > 2:148238/1‑139 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATATTAATAATCTTTTGaa > 2:89116/1‑138 (MQ=255) tGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATATTCAATAATCATTAAACTCAGCTTTTGaa > 8:130974/1‑138 (MQ=255) gCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGCCCCCTGGCAGTTTGCCAAGCTCATCGCGTTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAatat > 5:68982/1‑139 (MQ=255) gCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCCCTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAatat > 2:52749/1‑139 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTccg > 2:163626/1‑94 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTccg < 1:163626/94‑1 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa > 6:98157/1‑110 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa < 5:98157/110‑1 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTc > 3:49769/1‑139 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTc > 1:250422/1‑139 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCGTTTGAATATTTTTc > 5:232226/1‑139 (MQ=255) cACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAAACTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTc > 4:88294/1‑139 (MQ=255) cggaaCGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 2:175735/136‑1 (MQ=255) cGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 4:59171/139‑1 (MQ=255) cGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 4:88861/139‑1 (MQ=255) cGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 1:84353/139‑1 (MQ=255) cGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 5:304258/139‑1 (MQ=255) cGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTttatta < 2:250422/139‑1 (MQ=255) aCGGATACCACTACGTTTGCGGTCTTTCTCCCGACTCCTGGCAGTTTGAc > 5:28580/1‑50 (MQ=255) aCGGATACCACTACGTTTGCGGTCTTTCTCCCGACTCCTGGCAGTTTGAc > 5:32775/1‑50 (MQ=255) aCGGATACCACTACGTTTGCGGTCTTTCTCCCGACTCCTGGCAGTTTGAc < 6:28580/50‑1 (MQ=255) aCGGATACCACTACGTTTGCGGTCTTTCTCCCGACTCCTGGCAGTTTGAc < 6:32775/50‑1 (MQ=255) aTACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATAttttt > 2:230137/1‑121 (MQ=255) aTACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATAttttt < 1:230137/121‑1 (MQ=255) aTACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGAtata > 3:43220/1‑138 (MQ=255) ccACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAAcc > 7:115886/1‑139 (MQ=255) cTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat < 6:244403/139‑1 (MQ=255) cTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat < 1:158240/139‑1 (MQ=255) tACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgatt > 2:203902/1‑139 (MQ=255) tACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgatt > 2:269893/1‑139 (MQ=255) cGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGGTTTATTACGATATTATCCgattga > 6:122334/1‑139 (MQ=255) cGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat < 8:35390/136‑1 (MQ=255) cGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat > 7:35390/1‑136 (MQ=255) cGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCGTTTGAATATTTTTCGCTTTATTACGATATAAACCgattga > 6:297537/1‑139 (MQ=255) gCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa > 8:137041/1‑79 (MQ=255) gCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCaataa < 7:137041/79‑1 (MQ=255) gCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTaa > 8:74077/1‑139 (MQ=255) gCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTaa > 4:48618/1‑139 (MQ=255) gCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCGCCGCCGGAATCTTCAATAATCATTTTACTCAGCGTTTGAATATTTTTAGCTTTATTACGATATAAACAGATTGATTTaa > 3:215702/1‑139 (MQ=255) ggTCTTTCTCCAGACCCCTGGCAGTTTGACAAGCTCATCTCGGTCTCTCTGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTAata < 7:130974/139‑1 (MQ=255) tCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTAATATc < 1:203902/139‑1 (MQ=255) cTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTtct < 2:285267/55‑1 (MQ=255) cTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTtct > 1:285267/1‑55 (MQ=255) gACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat > 4:136182/1‑117 (MQ=255) gACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCgat < 3:136182/117‑1 (MQ=255) | TTTTCAACTTCCAGAACCGAGTCCTTCCACCGGCAAATGCCCAATCGTTTGCTGACTCTCTCCACATGGGTATCTACGGCTATGGCCGGCACGCCAAACGCAACGGATACCACTACGTTTGCGGTCTTTCTCCCGACCCCTGGCAGTTTGACAAGCTCATCGCGGTCTCTCGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTACTCAGCTTTTGAATATTTTTCGCTTTATTACGATATAAACCGATTGATTTAATATC > BS168_reference_genome/2344927‑2345189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |