Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	2,439,190	C→T	6.6%	E222K (GAG→AAG)	spoVAF ←	stage V sporulation protein AF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,439,190	0	C	T	6.6%	193.9 / 2.0	61	E222K (GAG→AAG)	spoVAF	stage V sporulation protein AF
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base T (2/2);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

GTAATGATCACACTCGGCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAATCCGTCTTTGACCG  >  BS168_reference_genome/2439054‑2439316                                                                                                                                         |                                                                                                                               gTAATGATCACACTCGGCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGa                                                                                                                              <  2:81269/139‑1 (MQ=255)               cGGCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGc                                                                                                                 >  5:309057/1‑138 (MQ=255)               cGGCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTATGATTAACAATGAATTCCTCGACAGTTTTGTCTGc                                                                                                                 >  1:212499/1‑138 (MQ=255)                ggCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGc                                                                                                                 <  4:6467/137‑1 (MQ=255)                ggCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGc                                                                                                                 <  2:8594/137‑1 (MQ=255)                          ccAATATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATc                                                                                                      >  4:294202/1‑138 (MQ=255)                            aCTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCAt                                                                                                   <  3:24622/139‑1 (MQ=255)                            aCTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCAt                                                                                                   <  3:126510/139‑1 (MQ=255)                            aCTATAATGAAAACATGGCCTTCTAGTACATGATTTGCGGCCAGATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCAt                                                                                                   <  2:177306/139‑1 (MQ=255)                                 aaTGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACa                                                                                              >  2:298701/1‑139 (MQ=255)                                   tGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACAt                                                                                             >  5:73002/1‑138 (MQ=255)                                      tAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAAt                                                                                         >  6:113979/1‑139 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTTGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATg                                                                                        >  8:165818/1‑138 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTTGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATg                                                                                        >  8:35684/1‑138 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATgg                                                                                       >  2:117216/1‑139 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATg                                                                                        >  8:59439/1‑138 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATg                                                                                        >  5:110313/1‑138 (MQ=255)                                        aCATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATg                                                                                        >  6:4863/1‑138 (MQ=255)                                              ccTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGa                                                                                      <  4:271999/134‑1 (MQ=255)                                              ccTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGa                                                                                      >  3:271999/1‑134 (MQ=255)                                                 tCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAAttt                                                                              >  4:74616/1‑139 (MQ=255)                                                   tAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCt                                                                            >  7:144400/1‑139 (MQ=255)                                                                    ccACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAAc                                                           <  3:261001/139‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTTGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGAt                                                <  7:165818/136‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGGCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAg                                             >  7:257562/1‑139 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGAt                                                <  1:298701/136‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGAt                                                <  5:93587/136‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGAt                                                <  6:73002/136‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCa                                              <  7:59439/138‑1 (MQ=255)                                                                                  tCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAg                                             >  3:273799/1‑139 (MQ=255)                                                                                      tataGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGcc                                                                                                                >  6:51226/1‑68 (MQ=255)                                                                                      tataGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGcc                                                                                                                <  5:51226/68‑1 (MQ=255)                                                                                      tataGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCtat                                         <  5:113979/139‑1 (MQ=255)                                                                                      tataGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCtat                                         <  3:74616/139‑1 (MQ=255)                                                                                          gcgcACGATCGGAATAGGATTATAGCTTTGATTAACTATGAATTCCTCGACCGTTTTGTCTGCCTTTGTTAATACATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCTGATCCTGATCATCTATAtc                                      <  6:304780/138‑1 (MQ=255)                                                                                          gcgcACGAGCGGAAAAGGATTATAGCTTTGATTAACTATGAATTCGTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCATTGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtc                                      <  2:212499/138‑1 (MQ=255)                                                                                          gcgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtc                                      <  6:309057/138‑1 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGa                                                                                      >  5:288232/1‑89 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGa                                                                                      <  6:288232/89‑1 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAAc                                                           >  4:81810/1‑116 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAAc                                                           <  3:81810/116‑1 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtctt                                    >  5:142624/1‑139 (MQ=255)                                                                                           cgcACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtctt                                    >  1:104450/1‑139 (MQ=255)                                                                                             cACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTAGAtcttc                                   >  2:98182/1‑138 (MQ=255)                                                                                              aCGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCta                                 <  4:273799/139‑1 (MQ=255)                                                                                                gAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCtata                               >  5:48970/1‑139 (MQ=255)                                                                                                gAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCtata                               >  5:303522/1‑139 (MQ=255)                                                                                                gAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCtata                               >  7:245749/1‑139 (MQ=255)                                                                                                      aaaaGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACaa                         <  4:248478/139‑1 (MQ=255)                                                                                                      aaaaGGAATATAGCTTTGATTAACAATGAATTCCTAGTCAGATTTGTCTGCCATTGTTAATCCATCGACATCTATGGATGCAATTTCTTTTTCTACGATATCAACCAGATCCGGATCAGCTATATCTTCTATATAACaa                         <  6:255647/139‑1 (MQ=255)                                                                                                                taGCTTTGATTAACAATGAATTCCTTGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAAt               <  7:35684/139‑1 (MQ=255)                                                                                                                taGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAAt               <  1:117216/139‑1 (MQ=255)                                                                                                                taGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAAt               <  2:104450/139‑1 (MQ=255)                                                                                                                taGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAAt               <  6:142624/139‑1 (MQ=255)                                                                                                                taGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAAt               <  6:110313/139‑1 (MQ=255)                                                                                                                     ttGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCtat                                         <  3:307657/108‑1 (MQ=255)                                                                                                                     ttGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCtat                                         >  4:307657/1‑108 (MQ=255)                                                                                                                      tGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAATCCGTc          <  8:257562/138‑1 (MQ=255)                                                                                                                             cAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAATCCGTCTTTGACCg  >  2:142537/1‑139 (MQ=255)                                                                                                                                  aaTTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtc                                      >  3:80427/1‑98 (MQ=255)                                                                                                                                  aaTTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATAtc                                      <  4:80427/98‑1 (MQ=255)                                                                                                                                         |                                                                                                                               GTAATGATCACACTCGGCGATGTGTCCACTATAATGATAACATGGCCTTCTAGAACATGATTTGCGGCCACATCCGGTCTTTCTGTATAGCGCACGAGCGGAAAAGGATTATAGCTTTGATTAACAATGAATTCCTCGACAGTTTTGTCTGCCATTGTTAATCCATCGACATCAATGGATGCAATTTCTTTTTCTACGATCTCAACCAGATCCGGATCAGCTATATCTTCTATATAACAAATGCTTAAATCCGTCTTTGACCG  >  BS168_reference_genome/2439054‑2439316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑