Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 3,139,266 T→C 79.0% intergenic (‑40/‑165) rpmEB ← / → ythA ribosomal protein L31/putative cytochrome bd menaquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,139,2660TC79.0% 79.3 / 15.0 38intergenic (‑40/‑165)rpmEB/ythAribosomal protein L31/putative cytochrome bd menaquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base T (2/6);  new base C (11/19);  total (13/25)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ATGTTTTCGTAGAGGTGCTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCAAATAATAAACCCAAAA  >  BS168_reference_genome/3139136‑3139391
                                                                                                                                  |                                                                                                                             
aTGTTTTCGTAGAGGTGCTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATaa                                                                                                                        <  2:266962/138‑1 (MQ=255)
       cGTAGAGGTGCTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACa                                                                                                                 <  1:76946/138‑1 (MQ=255)
                 cTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTtttactttat                                                                                                                          <  8:263764/119‑1 (MQ=255)
                 cTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTtttactttat                                                                                                                          >  7:263764/1‑119 (MQ=255)
                 cTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGt                                                                                                       <  5:131068/138‑1 (MQ=255)
                     gAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAAtc                                                                                                  <  7:87059/139‑1 (MQ=255)
                     gAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAAtc                                                                                                  <  6:141643/139‑1 (MQ=255)
                      aaaaCGGTAGCCGCTGTTGACATACTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTATATAACCTTACAAACGTTTTGTCAAtc                                                                                                  <  1:6705/138‑1 (MQ=255)
                            gTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTtat                                                                                           <  2:312866/139‑1 (MQ=255)
                             tAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAATCTTCTtata                                                                                          <  3:182462/139‑1 (MQ=255)
                                ccGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTACTTTCATGGGTATCTCCTTTCAATAAAACGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCTATCTTCTTatat                                                                                         >  4:300189/1‑137 (MQ=255)
                                  gCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCtt                                                                                     <  1:85611/139‑1 (MQ=255)
                                  gCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCtt                                                                                     <  8:303523/139‑1 (MQ=255)
                                  gCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCtt                                                                                     <  2:82459/139‑1 (MQ=255)
                                    tGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTTTCATTCTTCTTATttccttcc                                                                                   >  5:139988/1‑139 (MQ=255)
                                      ttGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCct                                                                                 <  3:166207/139‑1 (MQ=255)
                                           aTCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCct                                                                                 >  6:90466/1‑134 (MQ=255)
                                           aTCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCct                                                                                 <  5:90466/134‑1 (MQ=255)
                                             ccTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATACCCTTACAAACGTGTTGTCAATCTTCTTATATCCTTCCCTCTGttt                                                                           >  1:269239/1‑138 (MQ=255)
                                              cTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAAtc                                                                                                  <  2:62322/114‑1 (MQ=255)
                                              cTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAAtc                                                                                                  >  1:62322/1‑114 (MQ=255)
                                              cTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAAt                                                                                                   >  8:290456/1‑113 (MQ=255)
                                              cTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAAt                                                                                                   <  7:290456/113‑1 (MQ=255)
                                              cTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTca                                                                         <  7:154757/139‑1 (MQ=255)
                                              cTGAAATATCACTTTGTGGGTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTGTTGTCAATCTTCTTATATCCTTCCCTCTGTTTca                                                                         <  2:16264/139‑1 (MQ=255)
                                                gAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTcaca                                                                       >  4:130880/1‑139 (MQ=255)
                                                 aaaTATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTcaca                                                                       <  2:269239/138‑1 (MQ=255)
                                                             gtgGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCa                                                           <  1:235172/138‑1 (MQ=255)
                                                             gtgGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCa                                                           <  6:11669/138‑1 (MQ=255)
                                                                   cTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCAtt                                                    >  5:274072/1‑139 (MQ=255)
                                                                   cTTTGGATGAATTCATTCTTTCATGGGTATCACCTTTCAATAAATCGTAATAATTAAGTTTTATTTTATAACATTACAAACGTTTTGTCAATCTTCTTATATCATTCCCTCTGTTTCACATCTTAAACATCATGGAAtt                                                    >  4:175279/1‑139 (MQ=255)
                                                                             aTTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAgg                                          <  3:130880/139‑1 (MQ=255)
                                                                             aTTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAg                                           >  5:216229/1‑138 (MQ=255)
                                                                                                tctcCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAg                        <  6:265088/138‑1 (MQ=255)
                                                                                                      ttCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATATAACCGAGGAAGCa                  >  1:308864/1‑138 (MQ=255)
                                                                                                      ttCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTATTGTCAATCTTCTTATATCCTTCCATCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCa                  >  1:313290/1‑138 (MQ=255)
                                                                                                       tCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCaa                 >  4:99047/1‑138 (MQ=255)
                                                                                                       tCAATAAATCGTAATAATTACGTTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCa                  <  6:274072/137‑1 (MQ=255)
                                                                                                       tCAATAAATCGTAATAATTACGGTTTACTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCaa                 <  4:44493/138‑1 (MQ=255)
                                                                                                                     ttatTACGTTTTTCTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTACCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCAAATAATAAACCCaaaa  <  2:313290/137‑1 (MQ=255)
                                                                                                                                  |                                                                                                                             
ATGTTTTCGTAGAGGTGCTTAGAAAACGGTAGCCGCTGTTGACATCCTGAAATATCACTTTGTGGTTCTTTGGATGAATTCCTTCTTTCATGGGTATCTCCTTTCAATAAATCGTAATAATTACGTTTTATTTTATAACCTTACAAACGTTTTGTCAATCTTCTTATATCCTTCCCTCTGTTTCACATCGTAAACCTCATGGCATTTAAGAATAGGAAGAATAATCTAACCGAGGAAGCAAATAATAAACCCAAAA  >  BS168_reference_genome/3139136‑3139391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑