Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,654	Δ1 bp	intergenic (+29/+96)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,654	0	T	.	100.0%	319.1 / NA	70	intergenic (+29/+96)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (36/34);  total (36/34)

AAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGA  >  BS168_reference_genome/2480526‑2480775                                                                                                                                 |                                                                                                                          aaacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCg                                                                                                                >  6:177095/1‑139 (MQ=255)  aacaaaACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCgg                                                                                                               <  6:379111/139‑1 (MQ=255)       aaCCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑Ctttt                                                                                                         >  2:323965/1‑139 (MQ=255)             aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                   >  1:218868/1‑139 (MQ=255)                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                            <  8:206487/139‑1 (MQ=255)                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                             >  3:19753/1‑138 (MQ=255)                    aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGATGAACa                                                                                             >  3:223605/1‑138 (MQ=255)                    aaCTACGAATAAGGCTCAACGGGACCGAAAGAATCTTATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAACAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                            <  7:345595/139‑1 (MQ=255)                     aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                            <  1:160582/138‑1 (MQ=255)                      atACTAATCAGGCGAAATGGGAACGAAATAATCTTATTTTCTTTTGGTGAAAGACGGGTTACACTGGTGGAACATATAATAATAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAg                                                                                          <  4:223605/138‑1 (MQ=255)                         cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGTAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                       <  5:53642/139‑1 (MQ=255)                            ctCAGGCTCAATGGTACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATaa                                                                                    <  4:271230/138‑1 (MQ=255)                               aGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACa                                                                                  <  5:177095/138‑1 (MQ=255)                                ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                <  1:267714/139‑1 (MQ=255)                                ggCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGGGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAt                                                                                <  6:281685/139‑1 (MQ=255)                                 gCTCAATGGGAACGAAAGATTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                               <  5:226424/139‑1 (MQ=255)                                      aTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCt                                                                           >  6:308866/1‑138 (MQ=255)                                                    aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                            >  4:14690/1‑139 (MQ=255)                                                     tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                            >  4:355881/1‑138 (MQ=255)                                                        gatgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                          >  1:325479/1‑137 (MQ=255)                                                        acagatCTTTTCGTGAAACACCGCATACACTGGTGCAACATAAAAAAACAAGAAAAAACCCGAAAAAGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTAAAACAATCTCCTTAATAACAAAACCCCCa                                                          <  2:178473/134‑1 (MQ=255)                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCaa                                                         <  5:308866/137‑1 (MQ=255)                                                         atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                       >  5:350071/1‑139 (MQ=255)                                                                 ttGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAagag                                               >  8:23064/1‑139 (MQ=255)                                                                   ggTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCt                                             >  1:116685/1‑139 (MQ=255)                                                                      gAATGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AATCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAGCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                          <  6:135018/139‑1 (MQ=255)                                                                       aaaGACGGCTTTCAGTGGTGGTACATATAATAAGAAGAAAAATGCCGAAAATGTTTAC‑ATGCATTTTCGG‑CTTTTTTACGCTGATCAAAGGTATAACTTTCTCCTTATTTAGAAAACCCGCAAAGCTTAAGAGCtgtct                                         >  1:178473/1‑135 (MQ=255)                                                                       aaaGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCgg                                         >  3:232/1‑139 (MQ=255)                                                                           accgCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                     <  3:84154/136‑1 (MQ=255)                                                                           aCGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGt                                     <  4:349233/139‑1 (MQ=255)                                                                           aCGGCTTACACTGGTGGAACATAAAATAAGAAGAAAACAGCCGAAAATGAATAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAACGCTTACGCGCTTCGGGGGt                                     <  3:346294/139‑1 (MQ=255)                                                                             ggCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGttt                                   <  8:73399/139‑1 (MQ=255)                                                                                    acTGGTGGAACATTTAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                            <  8:64887/139‑1 (MQ=255)                                                                                    acTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                            <  2:218868/139‑1 (MQ=255)                                                                                    acTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGa                            <  7:41385/139‑1 (MQ=255)                                                                                       ggtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTa                         >  7:66311/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AATCATTTTAGG‑CTTTTTTACGCTGAACAAATGTAAAACAATATCCTTAATAACAAAACCCCCAAAGCGTAAGAGCTTCGGGGGTTTCTATttattaa                        >  2:77148/1‑138 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                            >  4:43519/1‑71 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                            <  3:43519/71‑1 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATATCAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  7:13656/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                          <  3:87501/105‑1 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                          >  4:87501/1‑105 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGTGTTTCTATTGATTAc                        >  8:177267/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  5:32371/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        <  6:350071/139‑1 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  2:41188/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  7:369335/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  5:381939/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  5:303930/1‑139 (MQ=255)                                                                                        gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  8:319464/1‑139 (MQ=255)                                                                                        gtgaAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                        >  8:193935/1‑139 (MQ=255)                                                                                         tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                       >  8:48686/1‑139 (MQ=255)                                                                                         tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                       >  6:61956/1‑139 (MQ=255)                                                                                             aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                              >  4:169893/1‑64 (MQ=255)                                                                                             aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                              <  3:169893/64‑1 (MQ=37)                                                                                               atatAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                  >  1:272536/1‑138 (MQ=255)                                                                                                tataATAAGCAGAAAACCGCCTAAAATGTTGAC‑AAGCATTTTCGG‑CTTTTTTACGCTGCACAAAGGTATCACACTCTCCTTAATAACAAAACCCCCACAGCGTACGAGCGGCGGGGGTTTCTATTGATTACTTCATCCa                <  3:289253/139‑1 (MQ=255)                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CATTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                >  4:354097/1‑139 (MQ=255)                                                                                                tataATAAGAAGAAAAAAGCCGAAAATGTATAC‑AAGCATATTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                <  3:289256/139‑1 (MQ=255)                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt              <  7:319464/139‑1 (MQ=255)                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt              <  3:14690/139‑1 (MQ=255)                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt              <  7:48686/139‑1 (MQ=255)                                                                                                  taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAt               >  2:350370/1‑138 (MQ=255)                                                                                                  taataaGAAGAAAAAAGAAGAAAATGTTTAC‑CAGCATTTTCGG‑CTTTTTTAAGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCTGGGGTTTCTATTGATTACTTAATCAAtt              <  6:297136/139‑1 (MQ=255)                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc             <  3:242840/134‑1 (MQ=255)                                                                                                        gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc             >  4:242840/1‑134 (MQ=255)                                                                                                           gaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATgg      >  2:228462/1‑138 (MQ=255)                                                                                                            aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                      <  3:111439/121‑1 (MQ=255)                                                                                                            aaaaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACtt                      >  4:111439/1‑121 (MQ=255)                                                                                                              aaaaGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGa  <  2:116685/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                          AAACAAAACCTTATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGA  >  BS168_reference_genome/2480526‑2480775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑