BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,751	Δ1 bp	intergenic (+44/‑3)	rrnI‑5S → / → trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,751	0	C	.	100.0%	426.1 / NA	110	intergenic (+44/‑3)	rrnI‑5S/trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (47/63); total (47/63)

Only 100 of 110 total aligned reads displayed.

CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑C‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTA  >  BS168_reference_genome/165620‑165866

cGTTCCCATCCCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGGAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              <  4:276688/139‑1 (MQ=18)

cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              <  1:39911/139‑1 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGCCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  6:160053/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGCCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  3:301233/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  8:275796/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  1:69411/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  8:35826/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGGTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                              >  1:116613/1‑137 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGcg                                                                                                         <  4:301233/139‑2 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  7:94372/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  1:65826/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  6:30753/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTGCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  1:116273/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCGCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  2:144154/139‑1 (MQ=255)

      cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGGTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                        <  4:120820/139‑1 (MQ=255)

       aTACCGAACTCGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTAACATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGAAGCt                                                                                                         >  7:175085/1‑137 (MQ=18)

       aTACCGAACACGGAAGTTAAGCTCTTCAGCGACGATGGTAGTCTGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         >  5:288402/1‑137 (MQ=21)

             aaCACGGAAGTTAAGCTCTTCAGCTCCTATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCAAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 <  6:288402/139‑1 (MQ=25)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTCCCATGTTTGTTTATGACGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  6:118358/1‑137 (MQ=21)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         >  8:140350/1‑131 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  7:140350/131‑1 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  6:202794/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  8:773/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  7:252090/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  6:371026/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  3:387629/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 <  2:69411/139‑1 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 <  3:304402/139‑1 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTACGCAGTAGCTCAGTgg                                                                                                   >  7:224622/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑AATCCGCAGTAGCTCAGTgg                                                                                                   >  7:201405/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGGGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                   >  5:2407/1‑137 (MQ=255)

             aaCACGGAAGTGACGCTCTTCAGCGCCGATGGTAGTCGGGGGGTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACAATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 <  8:175085/139‑1 (MQ=21)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  1:95487/139‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  4:127506/139‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  7:235642/139‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  4:387629/139‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  1:303319/139‑1 (MQ=255)

                          aGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                        <  3:282444/119‑1 (MQ=255)

                          aGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                        >  4:282444/1‑119 (MQ=255)

                              ctTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          <  2:176786/129‑1 (MQ=255)

                              ctTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                          >  1:176786/1‑129 (MQ=255)

                              ctTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTaa                                                                                >  7:21545/1‑139 (MQ=255)

                              ctTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTaa                                                                                >  8:72634/1‑139 (MQ=255)

                              ctTCAGCGCCGATGGTAGTCGGGGGGTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTGTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGGGGTAGAGCTATCGGCTGTTaa                                                                                <  3:195025/139‑1 (MQ=37)

                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                              >  1:221974/1‑139 (MQ=255)

                                 ccgcgcCGTTGGTAGTCGGGGGGTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTaa                                                                                <  8:144075/134‑1 (MQ=37)

                                   gcgcCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           <  2:167191/139‑1 (MQ=255)

                                   gcgcCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           <  7:35826/139‑1 (MQ=255)

                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCTGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                     >  6:182035/1‑139 (MQ=255)

                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         >  1:244065/1‑103 (MQ=255)

                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  2:244065/103‑1 (MQ=255)

                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                     >  1:37332/1‑139 (MQ=255)

                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                     >  5:140905/1‑139 (MQ=255)

                                          tGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGt                                                                    <  7:194216/139‑1 (MQ=255)

                                               gTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGtt                                                               <  5:163937/139‑1 (MQ=255)

                                                  gggggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           >  5:392496/1‑124 (MQ=255)

                                                  gggggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           <  6:392496/124‑1 (MQ=255)

                                                    gggTTTCCCCCTGTGAGAGTAGGACGCCGGCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAAt                                                          <  2:392688/139‑1 (MQ=255)

                                                    gggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAAt                                                          <  3:364023/139‑1 (MQ=255)

                                                     ggTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGACGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCTGTCGTAGGTTCGAATc                                                         >  8:87377/1‑139 (MQ=37)

                                                             ccTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGc                                                 <  5:98637/139‑1 (MQ=255)

                                                              cTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                <  4:190893/139‑1 (MQ=255)

                                                              cTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                <  7:773/139‑1 (MQ=255)

                                                              cTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                <  2:4604/139‑1 (MQ=255)

                                                               tgggagaGTAGGCCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGTTCGAATCCTACCTGCgg                                               >  2:255918/4‑139 (MQ=21)

                                                                     agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                           >  7:164707/1‑73 (MQ=255)

                                                                          gACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCtt                                   >  6:331220/1‑139 (MQ=21)

                                                                             gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                <  3:157384/139‑1 (MQ=21)

                                                                             gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                <  3:163279/139‑1 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         >  5:372682/1‑65 (MQ=255)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                         <  6:372682/65‑1 (MQ=255)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 >  4:287941/1‑73 (MQ=38)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                 <  3:287941/73‑1 (MQ=38)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           <  7:29311/95‑1 (MQ=25)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                           >  8:29311/1‑95 (MQ=25)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                >  1:368473/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCa                                >  8:200246/1‑137 (MQ=21)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccattg                                      <  1:369690/131‑6 (MQ=18)

                                                                               cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccattg                                      >  2:369690/1‑126 (MQ=18)

                                                                                gccAAGCAGATATATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAg                             <  4:16148/139‑1 (MQ=18)

                                                                                    aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCt                           >  3:55747/1‑137 (MQ=21)

                                                                                    aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGCTAGAGCTATCGGCTGTTAACAGATCGGTCGTAGGTTCGAATCCTACGTGCGGAG‑CAATTGCTTCCATAGCt                           >  7:336890/1‑137 (MQ=21)

                                                                                    aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑AATCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTca                         <  3:268318/139‑1 (MQ=21)

                                                                                      cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTcagc                       <  6:140905/139‑1 (MQ=21)

                                                                                      cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTcagc                       <  2:368473/139‑1 (MQ=21)

                                                                                      cAGATTTATTAATTCTAACATGTTTGTGTATGAAGATTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTcagc                       <  8:201405/139‑1 (MQ=18)

                                                                                              ttaATTCTACCATGTTTTATTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGGTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGTTCGAATCCTACCTGCGGAGCC‑TTTGCTTCCATAGCTCAGCAGGTagag               <  5:182035/139‑1 (MQ=18)

                                                                                              ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTagag               <  5:371026/139‑1 (MQ=21)

                                                                                              ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTagag               <  7:72634/139‑1 (MQ=21)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCTCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCTATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGAAGGTAGAGCACtt          >  4:201202/1‑139 (MQ=18)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt          >  1:118313/1‑139 (MQ=21)

                                                                                                   tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt          >  5:123843/1‑139 (MQ=21)

                                                                                                    cTACCATGTTTGTTAATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACtt          <  6:73785/138‑1 (MQ=18)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc         <  4:165818/136‑1 (MQ=18)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa       <  7:156145/138‑1 (MQ=21)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa       <  3:163644/138‑1 (MQ=21)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                       <  8:62363/106‑5 (MQ=14)

                                                                                                       ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                       >  7:62363/1‑102 (MQ=14)

                                                                                                            tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa  <  2:221974/138‑1 (MQ=21)

                                                                                                            tttgtttATGAAGCTTTAAAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTTTCGGCTGTTAACGGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCC‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTa  <  7:97168/138‑1 (MQ=18)

CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑C‑ATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTA  >  BS168_reference_genome/165620‑165866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`