Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 376,025 (G)8→7 intergenic (‑227/‑7) hxlA ← / → hxlR 3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome376,0180G.100.0% 277.1 / NA 61intergenic (‑220/‑14)hxlA/hxlR3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (40/21);  total (40/21)

AGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375882‑376151
                                                                                                                                        |                                                                                                                                     
aGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggg                                                                                                                                     <  4:309893/139‑1 (MQ=255)
  ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  8:187948/139‑1 (MQ=255)
  ttACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  6:380100/139‑1 (MQ=255)
   tACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  7:133140/138‑1 (MQ=255)
    aCTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggg                                                                                                                                  >  1:133624/1‑138 (MQ=255)
    aCTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggggg                                                                                                                                  >  6:368102/1‑138 (MQ=255)
     cTTGGTGGTTACTGTGAGGATAGAAAATAATATTTCTTTAGCTTTTTTTTATGAGTTACTATAATGAAAAAATAGTATGCAAAAAAGTAAGTAAGTTATGATACGTTTCTAACATACAAAAATGTTACTAAggtgggga                                                                                                                                <  7:274117/139‑2 (MQ=255)
     cTTGGATGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  1:110597/139‑2 (MQ=255)
     cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTAATGAGTGAGTATAATGAAAAAAGAGTCTTCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  4:313330/139‑2 (MQ=255)
        ggaggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  6:267082/136‑2 (MQ=255)
         gaggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAgggtgggattc                                                                                                                             >  6:82414/1‑134 (MQ=255)
          aggaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTa                                                                                                                          <  8:15160/139‑1 (MQ=255)
               aCTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAg                                                                                                                     >  5:386747/1‑139 (MQ=255)
                   tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                  >  8:63639/1‑138 (MQ=255)
                    gaggagAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGa                                                                                                                >  5:87403/1‑139 (MQ=255)
                    gaggagAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGa                                                                                                                >  6:179007/1‑139 (MQ=255)
                      ggagAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGATAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATg                                                                                                              >  4:29129/1‑139 (MQ=255)
                        agagaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAATGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGga                                                                                                            >  4:339101/1‑139 (MQ=255)
                          agaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgac                                                                                                           >  7:101089/1‑138 (MQ=255)
                               aTCAGATTTCTTTCTCTTGATTGTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGGGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:39819/139‑1 (MQ=255)
                               aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:392850/139‑1 (MQ=255)
                                    aTTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAAAATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGtt                                                                                                >  6:200072/1‑139 (MQ=255)
                                      ttctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACa                                                                                                       >  8:310682/1‑130 (MQ=255)
                                      ttctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACa                                                                                                       <  7:310682/130‑1 (MQ=255)
                                        ctttcGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTaa                                                                                             <  5:342693/138‑1 (MQ=255)
                                        ctttaGATTGATTTTATTTGTGATTATAATGAAAAAAGAGTCTGCAAATAAGTAAGTAAGTGATGATACGTATCTAACATACAATAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTaa                                                                                             <  2:93005/138‑1 (MQ=255)
                                                 gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  8:225531/137‑1 (MQ=255)
                                                 gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  6:292167/137‑1 (MQ=255)
                                                 gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  8:101089/137‑1 (MQ=255)
                                                       aTGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAAtt                                                                              >  6:305332/1‑138 (MQ=255)
                                                             gagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   >  7:393434/1‑80 (MQ=255)
                                                             gagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  8:393434/80‑1 (MQ=255)
                                                              agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGATGGAATTATCGCTTg                                                                      >  8:111960/1‑139 (MQ=255)
                                                              agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        <  5:294574/137‑1 (MQ=255)
                                                              agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        >  6:294574/1‑137 (MQ=255)
                                                              agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTg                                                                      >  1:173467/1‑139 (MQ=255)
                                                                tataATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGATGGAATTAACGCTTg                                                                      >  2:371165/1‑137 (MQ=255)
                                                                     tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAg                                                                                   <  4:180559/119‑1 (MQ=255)
                                                                     tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAg                                                                                   >  3:180559/1‑119 (MQ=255)
                                                                     tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAt                                                               >  8:360721/1‑139 (MQ=255)
                                                                       aaaaaaGAGTCAGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTg                                                             <  2:381475/139‑1 (MQ=255)
                                                                          aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCg                                                          >  8:15403/1‑139 (MQ=255)
                                                                               gTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGTAGTGATTGGCGGTaaa                                                     >  3:184800/1‑139 (MQ=255)
                                                                                 cttaaaaaaaGTAAGTTATTGATGATACTTATATAAAATTCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAAt                                                    <  2:193376/134‑1 (MQ=255)
                                                                                 cTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATg                                                   >  1:12894/1‑139 (MQ=255)
                                                                                     aaaaaaGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGaaa                                               <  7:360721/139‑1 (MQ=255)
                                                                                         aagtaattaagtGATCATACGTATCTAACATACAAAAAGGCTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGCCAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAATGGCGGTAAATGGAAAATGc                                           <  7:242964/139‑1 (MQ=255)
                                                                                          agtaagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                          <  3:339101/139‑1 (MQ=255)
                                                                                                      atgatACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCa                              <  3:29129/139‑1 (MQ=255)
                                                                                                          tACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCAtt                            >  5:402893/1‑137 (MQ=255)
                                                                                                            cGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAg                         >  1:346177/1‑138 (MQ=255)
                                                                                                             gTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAgg                        >  4:139900/1‑138 (MQ=255)
                                                                                                              tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAGCGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGa                       >  4:28442/1‑138 (MQ=255)
                                                                                                              tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGa                       >  5:99674/1‑138 (MQ=255)
                                                                                                              tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGa                       >  3:203579/1‑138 (MQ=255)
                                                                                                              tATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAAGGGAAAATGCTCATTTTATGGCATTTAGGa                       >  5:114584/1‑138 (MQ=255)
                                                                                                                tCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGaa                      >  6:115842/1‑137 (MQ=255)
                                                                                                                    aCATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaaga                  <  6:402893/137‑1 (MQ=255)
                                                                                                                     cATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCtt                                                                       <  8:347452/83‑1 (MQ=255)
                                                                                                                     cATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCtt                                                                       >  7:347452/1‑83 (MQ=255)
                                                                                                                     cATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGTAAAAGAAgg               >  4:33867/1‑139 (MQ=255)
                                                                                                                     cATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAgg               >  2:150785/1‑139 (MQ=255)
                                                                                                                     cATACAAAAAGGTTACTAA‑GGGCGCGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAgg               >  8:81234/1‑139 (MQ=255)
                                                                                                                        aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  6:71779/1‑138 (MQ=255)
                                                                                                                        aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  6:278448/1‑138 (MQ=255)
                                                                                                                        aCAAAAAGGTTACTAA‑GGGGGGGAGTCTATTGAGCCGGATGGACGACAAATGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGACGGca             >  4:362442/1‑138 (MQ=255)
                                                                                                                          aaaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGcaca           >  8:308052/1‑138 (MQ=255)
                                                                                                                          aaaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGAAAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGcaca           >  6:101582/1‑138 (MQ=255)
                                                                                                                                 ttACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaaga                  <  5:145098/124‑1 (MQ=255)
                                                                                                                                 ttACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAaaga                  >  6:145098/1‑124 (MQ=255)
                                                                                                                                  tACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  2:402769/56‑1 (MQ=255)
                                                                                                                                  tACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     >  1:402769/1‑56 (MQ=255)
                                                                                                                                   aCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGTAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGAACAAAACGGTTc  >  1:234727/1‑138 (MQ=255)
                                                                                                                                   aCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTc  >  4:124079/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                     
AGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375882‑376151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑