Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	97.0%	193.5 / ‑0.3	67	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base T (24/41);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAA  >  BS168_reference_genome/165900‑166166                                                                                                                                            |                                                                                                                                  gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTat                                                                                                                                   <  4:345822/139‑2 (MQ=255) gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTat                                                                                                                                   <  5:389686/139‑2 (MQ=255)    tAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTatcc                                                                                                                                 >  2:47323/1‑135 (MQ=255)     aaaTGTATTATTACCAAGGTTTCTCATAAGGAGAACGCTGTTTTTATGGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAAGACCGTCGTCCGCTCCA‑TTTatcct                                                                                                                                >  3:272783/1‑134 (MQ=21)          tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACggg                                                                                                                          <  1:43024/139‑1 (MQ=255)                  ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGt                                                                                                                   >  8:134518/1‑138 (MQ=255)                    aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTTAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGCTGCC‑TTTATCCTAACGGGTTATTGTaaa                                                                                                                >  8:54768/1‑139 (MQ=21)                      ggTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTaa                                                                                                                              <  2:83947/123‑1 (MQ=255)                      ggTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTaa                                                                                                                              >  1:83947/1‑123 (MQ=255)                      ggTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAaatt                                                                                                              >  8:73199/1‑139 (MQ=255)                          tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAaatttaat                                                                                                          >  8:166945/1‑139 (MQ=255)                            tcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCGCCA‑TTTATACTAACGGGATATTTTAaattgaattg                                                                                                        >  4:91014/1‑139 (MQ=21)                                  ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                                  <  1:30708/139‑1 (MQ=255)                                  ggAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                                  <  7:368896/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                               <  2:234247/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                               <  1:258199/139‑1 (MQ=255)                                     gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                               <  5:312881/139‑1 (MQ=255)                                       aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             <  3:80173/139‑1 (MQ=255)                                        aGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             >  4:13146/1‑138 (MQ=255)                                        aGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                             >  4:159609/1‑138 (MQ=255)                                          cgttttttAATGCGATATGCGGAAGTAGTTCAGTTGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGTTTCGAATCCCGGCTTCCGCTCCA‑TTTATCCTAACGTGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  6:399492/137‑1 (MQ=21)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  6:13182/139‑1 (MQ=255)                                          cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                          <  7:73199/139‑1 (MQ=255)                                             tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCTGGTTCGAATACCGGCTTGCGCTGCA‑TTTATACTAACGGGTTATTGTAAAATTCTTTGCGCCCGGAGCGCAATTg                                                                                       >  6:46300/1‑139 (MQ=17)                                             tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTGGAATCCCCGCTTACCCACCATTTTT‑ACTAACGGGATATTTTTAAATGAATTTAGCCCCTAGCACAAATg                                                                                       >  1:175673/1‑139 (MQ=12)                                             tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAt                                                                                         <  1:228037/137‑1 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGat                                                                                                                        >  7:346905/1‑99 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGat                                                                                                                        <  8:346905/99‑1 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                                >  3:113408/1‑139 (MQ=255)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTGCGCGCCC‑TTTATCCTAACGGGATATTGTTAAATTAATTTCGCCCGGAGCTCAATTggtgtgag                                                                                >  3:235736/1‑133 (MQ=18)                                                    tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATACCGTCTTCCGCGCCA‑ATTATCCTAACGGGATATTGTAAATTGAAATGCGCCCGTAGCTCAATTggg                                                                                     >  3:335273/1‑133 (MQ=18)                                                     gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                                <  3:13146/138‑1 (MQ=255)                                                     gCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATagag                                                                                <  5:375667/138‑1 (MQ=255)                                                               gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                     <  8:259938/139‑1 (MQ=255)                                                               gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                     <  7:134518/139‑1 (MQ=255)                                                               gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTAGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                                     <  7:90302/139‑1 (MQ=255)                                                                      ttccggggTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  8:254817/133‑1 (MQ=255)                                                                      ttCAGTGGTAGAAGACGACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTACCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTTACTACGGATCaa                                                              <  8:15009/139‑1 (MQ=37)                                                                      ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                              <  7:166945/139‑1 (MQ=255)                                                                      ttCAGTGGCAGAACTCGACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCGA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGACCGTAGCTCAATTGGATAGAGGGTTTGACTACGGATCaa                                                              <  6:169499/139‑1 (MQ=25)                                                                           tGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGt                                                         >  3:46729/1‑139 (MQ=255)                                                                            ggTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                        <  5:3135/139‑1 (MQ=255)                                                                            ggTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGCTCCC‑TTTATCCTAACGGGTTATTGTAAATTGAATTGCGCCCGGAGCTCAATTGGATTGAGCGTTGTGCGACGGCTCGAAAGGtt                                                        >  8:344198/1‑139 (MQ=21)                                                                             gTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                        <  7:14165/138‑1 (MQ=255)                                                                                gAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATACTAACGGGATAATGTAAATTTAATTTCGCCCCTAGCTCAATTTGATTGAGCGTTTGACTACGGATAAAAAGGTTAggg                                                    >  8:14165/1‑139 (MQ=25)                                                                                     ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACggg                                                                                                                          >  6:184906/1‑64 (MQ=255)                                                                                     ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACggg                                                                                                                          <  5:184906/64‑1 (MQ=255)                                                                                      gacagtGCCATGGGGGGGGGCGCGGGTTCGAATCCCTTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  5:307960/134‑1 (MQ=25)                                                                                      cagcgtGCCACGGTGGGGGGCGCGGGTTCGAATCCCGTATTCCGCTCCA‑TTTATCCTTACGGGATTTTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGTCTACGGATCAAAAGGTTAGGGGTTCGa                                              <  4:272783/134‑1 (MQ=21)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  1:352434/139‑1 (MQ=255)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  3:91014/139‑1 (MQ=255)                                                                                      caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCGTCCGCTCCATTTTT‑ACTAACGGGTTATTTTAAATTGAATTTCGCCCGGAGGGCAATTTGTGAGGGCGTTTTAATACGGGTCAAAAGGTTAGGGGTTCGa                                              >  6:37835/1‑139 (MQ=16)                                                                                      caccTTGCCAAGGGGGGGGTCGCGGGTGCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                              <  4:296475/139‑1 (MQ=255)                                                                                        ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                            >  8:365753/1‑139 (MQ=255)                                                                                           tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAaattgaattg                                                                                                        >  6:229241/1‑76 (MQ=255)                                                                                           tGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAaattgaattg                                                                                                        <  5:229241/76‑1 (MQ=255)                                                                                             ccAAGGTGGGGGTCGCGGATTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCctct                                       >  6:152015/1‑139 (MQ=255)                                                                                                 ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                   <  2:337562/139‑1 (MQ=255)                                                                                                 ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCggg                                   <  4:235736/139‑1 (MQ=255)                                                                                                   tGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTATATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcg                                 <  4:13042/139‑1 (MQ=255)                                                                                                   tGGGGGTCGCGGGGTCGAATCCCGTCGTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTGCGACTCCTCTCGGgcg                                 <  7:156790/139‑1 (MQ=25)                                                                                                     ggggTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGGCTACGGATCAAAAGGTTAGGGGTTCGCCTCCGCTCGGgcgcg                               >  3:8895/1‑139 (MQ=25)                                                                                                      gggTCGCGGGTTCGAATCCCCTCTTACGCTCCA‑ATTATGAAAACGGGATATTTTAAATTGAATTGAGACCGTATCTCAAATGGAAAGAGCCGTTGACAACGGATAAAAAAGTTATGGGTTAGACTACTCACGGgctgtc                              >  6:307960/1‑135 (MQ=255)                                                                                                        gTCGCGGGTTCGAATCCCGGCTTCCGCTCCA‑TTTATCCTACCGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                            <  1:163480/139‑1 (MQ=255)                                                                                                             gggTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCt                       <  5:276439/139‑1 (MQ=255)                                                                                                               gTTCGAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCttt                     <  4:113408/139‑1 (MQ=255)                                                                                                                   gAATCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaa                  <  4:177333/138‑1 (MQ=255)                                                                                                                    aaTCCCGTCTTCCGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata                <  7:365753/139‑1 (MQ=255)                                                                                                                                cGCTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAgg     <  4:335273/138‑1 (MQ=255)                                                                                                                                  cTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCGTCTCGGGCGCGCCAGATCTTTTATTAGAGTAGATAGGaat  >  5:132708/1‑138 (MQ=17)                                                                                                                                  cTCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGaa   >  1:281395/1‑138 (MQ=255)                                                                                                                                            |                                                                                                                                  GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGCTCCA‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGATAGGAAA  >  BS168_reference_genome/165900‑166166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑