Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,569,011	G→A	V30I (GTA→ATA)	ylbG →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,569,011	0	G	A	100.0%	281.0 / NA	76	V30I (GTA→ATA)	ylbG	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/33);  total (43/33)

GTCTCGTCTTTTACATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATGTATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTGGAATTC  >  BS168_reference_genome/1568881‑1569145                                                                                                                                   |                                                                                                                                       ctctcgtctTATACATATAAGAGAAAACAGGCAGGTGATTGTTTTTGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCaaaa                                                                                                                                <  2:86608/138‑1 (MQ=255)    tcgtctTTTACATATAATAGAAAACAGGCAGGTGATTTTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTAGGGAATTTGCACTATATATAAAAACg                                                                                                                              >  1:298645/1‑138 (MQ=255)           ttACATATAAGAGAAAACAGGCAGGTGATTGTTTTGTAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTaaa                                                                                                                        <  2:288856/137‑1 (MQ=255)           ttACATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTaaa                                                                                                                        <  5:281051/137‑1 (MQ=255)            tACATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAat                                                                                                                      <  3:389575/138‑1 (MQ=255)              cATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATg                                                                                                                   <  7:387707/139‑1 (MQ=255)              cATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATg                                                                                                                   <  1:306130/139‑1 (MQ=255)                tataAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTc                                                                                                                 <  3:116673/139‑1 (MQ=255)                   aaGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTACATATCAAAACGATTAAAATATGTCgta                                                                                                              <  2:250446/139‑1 (MQ=255)                    agagAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  1:29560/138‑1 (MQ=255)                    agagAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  7:133058/138‑1 (MQ=255)                        aaaaCAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATg                                                                                                                   >  8:117787/1‑129 (MQ=255)                        aaaaCAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATg                                                                                                                   <  7:117787/129‑1 (MQ=255)                               gcaggTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgtact                                                                                                       <  8:102163/134‑1 (MQ=255)                               gcaggTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgtact                                                                                                       >  7:102163/1‑134 (MQ=255)                                     gATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGAc                                                                                            >  4:40959/1‑139 (MQ=255)                                             tGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgta                                                                                                         >  5:397581/1‑118 (MQ=255)                                             tGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgta                                                                                                         <  6:397581/118‑1 (MQ=255)                                              ggAGAATAGGCGCCAGGGCATTGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGa                                                                                    <  2:310709/138‑1 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAAc                                                                              >  3:352533/1‑138 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAAc                                                                              >  7:163625/1‑138 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGAACTGTGATATGGACCAAATTGAAAAAAc                                                                              >  1:364334/1‑138 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTAATGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             <  1:334803/139‑1 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATCTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             <  6:291818/139‑1 (MQ=255)                                                    tAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATATCGTACTGTACTGTGATATGGACCAAATTGAAAAAAc                                                                              >  5:291818/1‑138 (MQ=255)                                                         gcCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGa                                                                        >  1:9567/1‑139 (MQ=255)                                                            aGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGAc                                                                       >  3:55944/1‑137 (MQ=255)                                                            aGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGAc                                                                       >  2:129529/1‑137 (MQ=255)                                                                       gtgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgaa                                                                                                              >  2:375890/1‑85 (MQ=255)                                                                       gtgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgaa                                                                                                              <  1:375890/87‑3 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgta                                                                                                         <  3:53142/91‑1 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgtactgta                                                                                                         >  4:53142/1‑91 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  8:179036/86‑1 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  7:179036/1‑86 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGa                                                                        >  1:170686/1‑124 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGa                                                                        <  2:170686/124‑1 (MQ=255)                                                                        tgtATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTa                                                         >  5:222296/1‑139 (MQ=255)                                                                          tATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACt                                                       >  1:13014/1‑139 (MQ=255)                                                                                cACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTtg                                                 >  1:372502/1‑139 (MQ=255)                                                                                    cccTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGa                                              >  6:97535/1‑138 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  4:348557/1‑63 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  7:349683/1‑63 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  7:276457/63‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  2:179100/63‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  1:179100/1‑63 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  8:276457/1‑63 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  3:348557/63‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              >  4:385784/1‑63 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  8:349683/63‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCgta                                                                                                              <  3:385784/63‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGATAAAACAATGGACAAGATTGCGTCTGACTCATTTGTGAAAAAAGTGGAGc                                  >  5:213168/1‑139 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             <  5:318847/96‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             <  1:285582/96‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             >  2:285582/1‑96 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACa                                                                             >  6:318847/1‑96 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTg                                      <  4:146202/135‑1 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTg                                      >  3:146202/1‑135 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGc                                  >  3:208805/1‑139 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGc                                  >  2:131538/1‑139 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGc                                  >  3:22445/1‑139 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGc                                  >  5:247097/1‑139 (MQ=255)                                                                                               agCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGc                                  >  8:30470/1‑139 (MQ=255)                                                                                                gCAAAATGCTGAGGAATTTCGGGAATGTGCACTATATATGAAAACGATTAAAATATGTCGTACTGTACTGTTATATTGAGCAAATTGAATAAACAATTGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGTGcc                                 <  5:198995/139‑1 (MQ=255)                                                                                                gCAAAATGCTGAGGAAGTTCTGGAATGTGCACTATATATAAAATCGATTAAAATATGTCGTACTGTACTGTTATATGTAACAAATTGTAAAAACAATGTACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGcc                                 <  3:255535/139‑1 (MQ=255)                                                                                                gCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGcc                                 <  4:22445/139‑1 (MQ=255)                                                                                                           aGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATaacc                      >  5:376459/1‑137 (MQ=255)                                                                                                           aGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAAc                      >  7:85588/1‑139 (MQ=255)                                                                                                           aGGAAGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAAc                      >  5:60936/1‑139 (MQ=255)                                                                                                              aaGTTCGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGt                   >  6:182000/1‑139 (MQ=255)                                                                                                                   cGGGAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGttttt               <  4:55944/138‑1 (MQ=255)                                                                                                                     ggAATGTGCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTaa             >  8:89317/1‑138 (MQ=255)                                                                                                                         tgtgCAATATATATCAAAACGATTAAAATATCTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCTTCTTACTCATTTGTGAAAAAAGTGGAGCATTCTTATAAACCGTTTTTAAAAtac        >  7:327554/1‑137 (MQ=255)                                                                                                                           tgCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTgg       >  5:370005/1‑138 (MQ=255)                                                                                                                           tgCACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTgg       >  5:367266/1‑138 (MQ=255)                                                                                                                             cACTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTGGTAt    >  3:61652/1‑139 (MQ=255)                                                                                                                              aCTATATATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTGGAAtt   <  6:60936/139‑1 (MQ=255)                                                                                                                                tatataTCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTGGAATTc  >  4:40412/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                                       GTCTCGTCTTTTACATATAAGAGAAAACAGGCAGGTGATTGTATTGGAGAATAGGCGCCAGGGCATGGTTGTGTATCTGCACTCCCTAAAGCAGAGCAAAATGCTGAGGAAGTTCGGGAATGTGCACTATGTATCAAAACGATTAAAATATGTCGTACTGTACTGTGATATGGACCAAATTGAAAAAACAATGGACAAGATTGCGTCTTACTCATTTGTGAAAAAAGTGGAGCCTTCTTATAAACCGTTTTTAAAATTGGAATTC  >  BS168_reference_genome/1568881‑1569145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑