Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,435,685	T→C	E36G (GAA→GGA)	ppiB ←	peptidyl‑prolyl isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,435,685	0	T	C	100.0%	346.4 / NA	95	E36G (GAA→GGA)	ppiB	peptidyl‑prolyl isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (67/28);  total (67/28)

TATGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTTCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTATTTTAATTT  >  BS168_reference_genome/2435554‑2435820                                                                                                                                    |                                                                                                                                        tATGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGc                                                                                                                                  <  2:361247/139‑1 (MQ=255)   tGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCCTCCGCCTTGGCTGACGAAGCCAGGGATCACTCGCTGGAATGTTCGCCCGGCATAACAGCCTCCGTTCGc                                                                                                                                  >  1:132795/1‑137 (MQ=255)   tGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCaa                                                                                                                                <  7:278673/139‑1 (MQ=255)   tGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCACGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCaa                                                                                                                                >  1:275269/1‑139 (MQ=255)     cGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGc                                                                                                                              >  3:133224/1‑139 (MQ=255)     cGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGc                                                                                                                              >  1:249846/1‑139 (MQ=255)       ggTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGCTCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCtt                                                                                                                            >  4:244048/1‑139 (MQ=255)           gCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCCTTTc                                                                                                                         >  2:315760/1‑138 (MQ=255)           gCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCACGTCATAAAAGCCTCCGTTCGCACGCTTTTc                                                                                                                         >  6:322636/1‑138 (MQ=255)           gCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCAGTGCGGGCATCCGCCTTGGCTGACGACGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTc                                                                                                                         >  5:159741/1‑138 (MQ=255)            ccTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCa                                                                                                                        <  1:256463/138‑1 (MQ=255)            ccTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCAGAACCAGTTCCGTGCGGGCATCCGCCTTGGCAGACGAAGCCAGGTATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCa                                                                                                                        <  6:326658/138‑1 (MQ=255)                   tttCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTg                                                                                                                 >  8:359893/1‑138 (MQ=255)                       acaTTAGATTGAGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAAc                                                                                                             <  8:349291/138‑1 (MQ=255)                                  tgtATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTg                                                                                                  <  4:117795/138‑1 (MQ=255)                                  tgtATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTg                                                                                                  >  1:235076/1‑138 (MQ=255)                                       cccGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCtt                                                                                            >  1:315195/1‑139 (MQ=255)                                        ccGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCtt                                                                                            <  2:216359/138‑1 (MQ=255)                                           gACCGCCTGTACCAGTTCCGTGCTGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTAGCAAGCTTTTCAAAGTTTGCAACAGTTCGAGGTGCAGCTTCAg                                                                                         >  2:132660/1‑138 (MQ=255)                                           gACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAgg                                                                                        >  6:7468/1‑139 (MQ=255)                                            aCCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCaa                                                                                                              >  7:335752/1‑116 (MQ=255)                                            aCCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCaa                                                                                                              <  8:335752/116‑1 (MQ=255)                                            aCCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCATCAGTTCCAGGTGCCGCTTCAGGa                                                                                       >  1:283239/1‑139 (MQ=255)                                            aCCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGa                                                                                       >  7:105708/1‑139 (MQ=255)                                              cGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGCCGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGAt                                                                                      >  6:206143/1‑138 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAACGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATa                                                                                     >  8:350272/1‑137 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCa                                                                                                      <  4:202218/120‑1 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCa                                                                                                      >  3:202218/1‑120 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATaaa                                                                                   >  2:31670/1‑139 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATaaa                                                                                   >  5:117784/1‑139 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATa                                                                                     >  4:19096/1‑137 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATa                                                                                     >  4:299379/1‑137 (MQ=255)                                                ccTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATa                                                                                     >  2:232509/1‑137 (MQ=255)                                                    tACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTTGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTc                                                                               <  7:346191/139‑1 (MQ=255)                                                          ttCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAAtt                                                                          >  3:175917/1‑138 (MQ=255)                                                          ttCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAAtt                                                                          >  5:400112/1‑138 (MQ=255)                                                          ttCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTc                                                                         <  7:80876/139‑1 (MQ=255)                                                          ttCCGTGCGGGCATCCCCCTTTGCTGACGAAGCCAGGGATCACACGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAATCTTTTCAAAGTTTGCAACAGTTCCATGTGCAGCGTCAGGATAAAGTTCAAAtt                                                                          >  8:346191/1‑138 (MQ=255)                                                              gTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAtt                                                                     >  2:298905/1‑139 (MQ=255)                                                              gTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAtt                                                                     >  7:210388/1‑139 (MQ=255)                                                              gTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCATCCGTTCGCAAGCTTTTAAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAtt                                                                     >  6:358762/1‑139 (MQ=255)                                                              gTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCACGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGTTGCAGCTTCAGGATACAGTTCcaattcaatt                                                                     >  6:17222/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCCGGGATCACTCGGTGGAATGTAAGCCCGTCATAAACGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTGCAGGATAAAGTTCAAATTCAAttt                                                                    >  2:68813/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  7:222585/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  6:79156/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  7:322809/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  6:143633/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCGTTTCAAAGTTTGAAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  7:184323/1‑139 (MQ=255)                                                               tGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCCGCGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTTCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAAttt                                                                    >  4:79746/1‑139 (MQ=255)                                                               tGCGGGCAACCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTTAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTACAGGAGCAGCTTCAGGATAAAGTTCAAATTAAAatt                                                                    >  4:147877/1‑139 (MQ=255)                                                                           ccTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGtctt                                                        <  5:322636/139‑1 (MQ=255)                                                                           ccTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCCTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGtctt                                                        <  1:315760/139‑1 (MQ=255)                                                                                gCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCtaa                                                    >  6:244930/1‑138 (MQ=255)                                                                                 cTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCtaat                                                   >  3:262402/1‑138 (MQ=255)                                                                                    aCGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGCGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATaaa                                                >  6:72231/1‑138 (MQ=255)                                                                                     cGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCATCAGTTCGAGGTGCAGCTTCAGGATAAAGTTGATATTCATTTTTGATGCCGTCTTCTAATaaaaa                                              >  7:262557/1‑139 (MQ=255)                                                                                          ccAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAAc                                         <  4:259183/139‑1 (MQ=255)                                                                                             gggATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTg                                      <  2:105725/139‑1 (MQ=255)                                                                                                 tCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGtttt                                  <  3:299379/139‑1 (MQ=255)                                                                                                 tCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGtttt                                  <  8:322809/139‑1 (MQ=255)                                                                                                    cTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCaa                               <  2:61647/139‑1 (MQ=255)                                                                                                    cTCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCaa                               >  8:253027/1‑139 (MQ=255)                                                                                                     tCGGTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAAt                              >  8:363043/1‑139 (MQ=255)                                                                                                       ggTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCaa                                                                                                              >  8:210493/1‑57 (MQ=255)                                                                                                       ggTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCaa                                                                                                              <  7:210493/57‑1 (MQ=255)                                                                                                       ggTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAAt                              <  7:359893/137‑1 (MQ=255)                                                                                                       ggTGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTg                            >  7:384618/1‑139 (MQ=255)                                                                                                        gTGGAATGTAAGCCCGACATAAAAGCCTCCGTTCGCTAGCTTTTCAAAGTTTGCAAAAGTTCCAGTTGCAGCTTCAGGATAAATTTCAAATTCAATTTTGTTACAGTCTTCTAATAAAAATTAACCTGTTTTAAATTg                            >  7:325603/1‑138 (MQ=255)                                                                                                         tGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTccc                                                             <  2:349679/104‑1 (MQ=255)                                                                                                         tGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTccc                                                             >  1:349679/1‑104 (MQ=255)                                                                                                         tGGAATGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTa                          >  2:155930/1‑139 (MQ=255)                                                                                                            aaTGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGc                       <  5:244930/139‑1 (MQ=255)                                                                                                            aaTGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGc                       <  8:210388/139‑1 (MQ=255)                                                                                                              tGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTg                            <  2:26840/132‑1 (MQ=255)                                                                                                              tGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTg                            >  1:26840/1‑132 (MQ=255)                                                                                                              tGTAAGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGcc                      >  8:348003/1‑138 (MQ=255)                                                                                                                 aaGCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCACGATAAAGTTCAAATTCAAATTTGTTCCCGTCTTCTAATAAAATGTAACCTGTATACAATTGTATGCCTAca                  >  2:340181/1‑138 (MQ=255)                                                                                                                   gCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATTCCTActt                 >  6:172843/1‑138 (MQ=255)                                                                                                                   gCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActtt                >  2:55037/1‑139 (MQ=255)                                                                                                                   gCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActtt                >  5:143280/1‑139 (MQ=255)                                                                                                                   gCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActtt                >  6:72686/1‑139 (MQ=255)                                                                                                                   gCCCGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActt                 >  2:175077/1‑138 (MQ=255)                                                                                                                     ccGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActttct              <  1:31670/139‑1 (MQ=255)                                                                                                                     ccGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActttct              <  1:232509/139‑1 (MQ=255)                                                                                                                      cGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTActttctt             >  4:36161/1‑139 (MQ=255)                                                                                                                      cGTCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACATGTTTTCAATTGTATGACTAcgttctt             >  4:301372/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTa           >  2:40410/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTa           >  3:236869/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTa           >  2:124748/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTa           >  7:143991/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTa           >  6:166349/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGATTTCAATTGTATGCCTACTTTCTTTa           >  4:47044/1‑139 (MQ=255)                                                                                                                        tCATAAAAGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGGCTTCTAATAAAAAGTAACCTGTTTTCAATTGTAGGCCTActttcttga           >  6:166523/1‑137 (MQ=255)                                                                                                                             aaaGCCTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTATTTTa      <  6:400112/139‑1 (MQ=255)                                                                                                                                 ccTCCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTATTTTAAttt  <  1:55037/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TATGCGGGTTGCCTTCTGTTTCACATTTGATTGTGTATCCCGGACCGCCTGTACCAGTTCCGTGCGGGCATCCGCCTTGGCTGACGAAGCCAGGGATCACTCGGTGGAATGTAAGCCCGTCATAAAAGCCTTCGTTCGCAAGCTTTTCAAAGTTTGCAACAGTTCCAGGTGCAGCTTCAGGATAAAGTTCAAATTCAATTTTGTTCCCGTCTTCTAATAAAAAGTAACCTGTTTTCAATTGTATGCCTACTTTCTTTATTTTAATTT  >  BS168_reference_genome/2435554‑2435820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑