Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	376,018	Δ1 bp	100%	intergenic (‑220/‑14)	hxlA ← / → hxlR	3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	376,018	0	G	.	100.0%	154.3 / NA	37	intergenic (‑220/‑14)	hxlA/hxlR	3‑hexulose‑6‑phosphate synthase (HPS)/positive regulator of hxlAB expression (formaldehyde sensing)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (24/13);  total (24/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375882‑376151                                                                                                                                         |                                                                                                                                      aGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggg                                                                                                                                     <  8:193200/139‑1 (MQ=255)      cTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggggga                                                                                                                                <  1:2928/139‑2 (MQ=255)             gaTACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGATAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTACCATACAAAAAGCTTACTAA‑GGGGGGGATTCTAtt                                                                                                                        >  1:176032/1‑139 (MQ=255)                    tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   >  1:91436/1‑122 (MQ=255)                    tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                  >  4:9504/1‑138 (MQ=255)                    tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCg                                                                                                                  >  8:133890/1‑138 (MQ=255)                    tgAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGGCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggggg                                                                                                                                   <  2:91436/122‑1 (MQ=255)                         agagaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGga                                                                                                            >  8:49371/1‑139 (MQ=255)                           agaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggg                                                                                                                                     <  4:185604/113‑1 (MQ=255)                           agaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAggg                                                                                                                                     >  3:185604/1‑113 (MQ=255)                           agaCAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGgac                                                                                                           >  4:74567/1‑138 (MQ=255)                            gaCAATCAGATTTCTTTCGCTTGATTTTATGCGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACTTACAAATAGGTTACTAA‑GGGGGGGATTCTATAGAGCCGGATGgccga                                                                                                         >  6:205739/1‑139 (MQ=255)                                aTCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  3:18700/139‑1 (MQ=255)                                aTCAGATTGCTATCGCTTGATTTTATGAGTGAGTATAAATAAAAAAGAGTCTTCATAAATGTAAGTAACTGATGATACGTATCTAACATACAAAAAGGTTTCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACaaa                                                                                                     <  5:24233/139‑1 (MQ=255)                                                ttGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  7:57209/139‑1 (MQ=255)                                                attaTTTTATGTGGTAGTATAATGTATAAAGAGTCTGCAAAAAAGTAAGTAAGTGTTGAAACGTATCTATCATACAAATAGTTTACTTA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  1:49122/136‑1 (MQ=255)                                                  gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  7:133890/137‑1 (MQ=255)                                                  gATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTgaga                                                                                     <  7:49371/137‑1 (MQ=255)                                                        aTGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAAtt                                                                              <  7:132557/138‑1 (MQ=255)                                                        aTGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAAtt                                                                              >  4:198034/1‑138 (MQ=255)                                                        aTGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAAtt                                                                              <  6:107579/138‑1 (MQ=255)                                                             tgagtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCt                                                                        <  7:4108/139‑1 (MQ=255)                                                               agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGga                                                                                                            >  5:199934/1‑101 (MQ=255)                                                               agtATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGga                                                                                                            <  6:199934/101‑1 (MQ=255)                                                                  ataATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCa                                                                    <  3:103298/138‑1 (MQ=255)                                                                      tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAAGTGTGAGAAGGAATTAACGCTGGCAGTGAt                                                               >  2:188564/1‑139 (MQ=255)                                                                      tGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATAGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAt                                                               >  3:173118/1‑139 (MQ=255)                                                                           aaaGAGTCTGCAAAAAAGTAAGTAAGTGATGAAACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTACCGCTTGCAGTGATTGGCg                                                          >  6:399/1‑139 (MQ=255)                                                                                 tCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTaatt                                                    >  8:23314/1‑137 (MQ=255)                                                                                             taagtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCa                                        >  3:112079/1‑139 (MQ=255)                                                                                               agtaagtGATGATACGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACTAAAGTTTTAATTGTGAGAAGGAATTTACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCagt                                      >  5:85871/1‑137 (MQ=255)                                                                                                            aCGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                          <  7:28710/122‑1 (MQ=255)                                                                                                            aCGTATCTAACATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCt                                          >  8:28710/1‑122 (MQ=255)                                                                                                                    aaCATACAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGTCATTTAGGAAaag                   >  4:170151/1‑137 (MQ=255)                                                                                                                         aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATTGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCTGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  2:34758/1‑138 (MQ=255)                                                                                                                         aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTACCGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  6:122009/1‑138 (MQ=255)                                                                                                                         aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  3:183366/1‑138 (MQ=255)                                                                                                                         aCAAAAAGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  2:132411/1‑138 (MQ=255)                                                                                                                           aaaaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGcaca           >  1:168310/1‑138 (MQ=255)                                                                                                                             aaaGGTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGAGTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACaaaa        >  6:219126/1‑139 (MQ=255)                                                                                                                                 gTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTATCGCTTGCAGTGATTGGCGTTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             <  2:61910/130‑1 (MQ=255)                                                                                                                                 gTTACTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGca             >  1:61910/1‑130 (MQ=255)                                                                                                                                    aCTAA‑GGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTc  >  7:23642/1‑138 (MQ=255)                                                                                                                                         |                                                                                                                                      AGTTACTTGGAGGATACTGTGAGGAGAGACAATCAGATTTCTTTCGCTTGATTTTATGAGTGAGTATAATGAAAAAAGAGTCTGCAAAAAAGTAAGTAAGTGATGATACGTATCTAACATACAAAAAGGTTACTAAGGGGGGGGATTCTATTGAGCCGGATGGACGACAAAAGGTTTAATTGTGAGAAGGAATTAACGCTTGCAGTGATTGGCGGTAAATGGAAAATGCTCATTTTATGGCATTTAGGAAAAGAAGGCACAAAACGGTTC  >  BS168_reference_genome/375882‑376151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑