| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 675,819 | C→T | 11.4% | L13L (TTG→TTA) | ydjJ ← | putative membrane associated potassium channel; prophage region 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 675,819 | 0 | C | T | 11.4% | 93.0 / 3.3 | 35 | L13L (TTG→TTA) | ydjJ | putative membrane associated potassium channel; prophage region 3 |
| Reads supporting (aligned to +/- strand): ref base C (17/14); new base T (2/2); total (19/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTAGAACCTTTGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAATTGAACGAATGTTTGATTTTACAG > BS168_reference_genome/675684‑675953 | aCTTAGAACCTTTGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCaa > 4:54995/1‑138 (MQ=255) gAACCTTTGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAACTCAATCATCCCCGCTGTCAAtaataa < 7:121018/139‑1 (MQ=255) aaCCTTTGCGTGGTCACCGCTGATTTTGCGGCCGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAAtaataa > 8:132649/1‑138 (MQ=255) tcgtttGCGTGGTCAGCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGAGAGGCTTCTTGTGATCGGGCCGGCACCGCTCTCTAACCTTAACGTGGTTCATAAATAATCAATCATCCCCGCTGTCAATAATaaa < 8:134167/135‑1 (MQ=255) ttGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGcc > 3:194380/1‑139 (MQ=255) ttGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGcc > 5:161220/1‑139 (MQ=255) ttGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACACGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGcc > 6:83517/1‑139 (MQ=255) tGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGcc < 6:85620/138‑1 (MQ=255) tGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCt > 1:183850/1‑139 (MQ=255) gTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCa < 4:194380/139‑1 (MQ=255) tGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCaa < 6:161220/139‑1 (MQ=255) tGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACATGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCaa < 1:113588/139‑1 (MQ=255) tCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAAtaataa < 8:120487/125‑1 (MQ=255) tCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAAtaataa > 7:120487/1‑125 (MQ=255) aTTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTTAATAATAAAAGTCCTGCAATGATATAACCCt > 8:105390/1‑139 (MQ=255) aTTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATACCCGCTGTTAATAATAAAAGGCCTGCAATGATATAACCCt > 6:188465/1‑139 (MQ=255) gCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCtcatt < 2:183850/138‑1 (MQ=255) ccAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCt > 7:50932/1‑138 (MQ=255) ggCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATACCGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCa < 5:179600/100‑1 (MQ=255) ggCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATACCGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCa > 6:179600/1‑100 (MQ=255) ggCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAg > 8:150456/1‑139 (MQ=255) aCAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGc > 1:46711/1‑139 (MQ=255) aCAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGc < 2:46711/139‑1 (MQ=255) tgtgATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTAt > 2:78753/1‑139 (MQ=255) cGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTttctt > 3:31634/1‑139 (MQ=255) gCACCGCTCTCTAACCATAACGTTGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTAtt < 4:31634/139‑1 (MQ=255) gCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTAtt < 8:50932/139‑1 (MQ=255) ctctctAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTAATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTATTGTCTATTTACttg > 4:128926/1‑138 (MQ=255) aCGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCt > 7:58821/1‑138 (MQ=255) gTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCATCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTATATTTCATTTTATCCTGAGTCTGa > 6:68734/1‑138 (MQ=255) tGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGGCTGaaa < 3:54995/139‑1 (MQ=255) cTGTTAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAATTGAACGAATGTTTGATTTTACag < 7:105390/139‑1 (MQ=255) cTGTTAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAAATGAACGAATGTTTGATTTTACag < 5:188465/139‑1 (MQ=255) cTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAATTGAACGAATGTTTGATTTTa > 7:219650/1‑136 (MQ=255) cTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAATTGAACGAATGTTTGATTTTACag < 1:78753/139‑1 (MQ=255) | ACTTAGAACCTTTGCGTGGTCACCGCTGATTTTGCGGCAGCCCTTCCACAGCCAGGCAGACAGGCATCTTGTGATCGGGCCGGCACCGCTCTCTAACCATAACGTGGTCCATAAAAAATCAATCATCCCCGCTGTCAATAATAAAAGGCCTGCAATGATATAACCCTCATTCATTTTGGTTGCCTCCCTTAAGTCAGCCTTCTTTTATTTTCTTCTCTATTTCCTTTTATCCTGAGTCTGAAACAATTGAACGAATGTTTGATTTTACAG > BS168_reference_genome/675684‑675953 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |