| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,073,672 | A→T | 100% | F16I (TTC→ATC) | scoC ← | transcriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,073,672 | 0 | A | T | 100.0% | 78.0 / NA | 24 | F16I (TTC→ATC) | scoC | transcriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (11/13); total (11/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGAAAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTTAATA > BS168_reference_genome/1073550‑1073804 | aTACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCttt > 1:35950/1‑138 (MQ=255)aTACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTc > 3:33056/1‑139 (MQ=255) tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca < 1:203681/139‑1 (MQ=255) tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca < 7:186644/139‑1 (MQ=255) tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca < 4:221889/139‑1 (MQ=255) aTAAAATTTTATGCTCATTATTATTCAGGTCATACTGTTTTATCCATTGCTGCCAATTCTTCTGGATCGATTTCCTAAGATACTTGCTAAGATGATTCATTTTATTGGTGATTACCAGAGCTTCTTTCACATCATAggg < 4:33056/139‑1 (MQ=255) aTAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAggg < 2:35950/139‑1 (MQ=255) aTAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAggg < 1:154362/139‑1 (MQ=255) tgCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTc < 3:76014/139‑1 (MQ=255) aGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCAc > 4:173129/1‑139 (MQ=255) tGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGc < 6:49637/82‑1 (MQ=255) tGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGc > 5:49637/1‑82 (MQ=255) ttGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt < 5:164111/139‑1 (MQ=255) tgctgcCTATCCTTCTCGATCGATTTCCAAATTGCCTTGCTAAGCTGAGGCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATGATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttgttttt < 1:29061/138‑1 (MQ=255) tgctgcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCtttttctttttc > 4:7700/1‑139 (MQ=255) tgctgcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCtttttctttttc > 5:137885/1‑139 (MQ=255) tgctgcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCtttttctttttc > 4:147183/1‑139 (MQ=255) gcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCta > 4:201229/1‑139 (MQ=255) ctcGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCaa < 6:137885/138‑1 (MQ=255) tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt < 3:217815/101‑1 (MQ=255) tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt > 4:217815/1‑101 (MQ=255) aGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTGTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAg > 7:133973/1‑138 (MQ=255) aGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAg > 6:215456/1‑138 (MQ=255) ggTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTtaata < 8:133973/138‑1 (MQ=255) | ATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGAAAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTTAATA > BS168_reference_genome/1073550‑1073804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |