| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,474,890 | C→T | 100% | L45L (TTG→TTA) | kre ← | regulator of transcription factor ComK function via modulation of mRNA stability |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,474,890 | 0 | C | T | 95.5% | 66.8 / ‑4.3 | 22 | L45L (TTG→TTA) | kre | regulator of transcription factor ComK function via modulation of mRNA stability |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (7/14); total (8/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT > BS168_reference_genome/1474768‑1475024 | ggCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGtttttt > 4:175944/1‑137 (MQ=255) gCATTTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag < 4:181334/139‑1 (MQ=255) gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag < 3:52459/139‑1 (MQ=255) aaaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGata < 3:4076/138‑1 (MQ=255) aaaaTAGTAGTCTCCGATTGAGATAACCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGata < 2:26062/138‑1 (MQ=255) gaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 2:70508/138‑1 (MQ=255) aaaTCGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 6:174148/139‑1 (MQ=255) aaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg < 3:175944/139‑1 (MQ=255) aaTAGTAGTCTCCGATTGAGATAAGCAAGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg < 4:176296/139‑1 (MQ=255) tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAATATGAAGCACTATTTTCTTTCCTTTACGTTCTTTGACCAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAggg > 1:70097/1‑139 (MQ=255) aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAATCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCgg > 3:189841/1‑139 (MQ=255) cACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt > 4:152407/1‑139 (MQ=255) gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa < 4:189841/139‑1 (MQ=255) tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa > 1:42075/1‑138 (MQ=255) tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa < 2:42075/138‑1 (MQ=255) ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCATTTACCTTCTTTGACAAGCTTTTGTAAATCCAGTTTTTTCTGCAGATATAGGTATTTAGTGTTGTTGTAGGTTTTTTCATg > 7:158765/1‑139 (MQ=255) cTGTTTGCTGAACCTTTGATTTTTTGAAAAATGAAGCCATATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc < 8:158765/139‑1 (MQ=255) cTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc < 4:173800/139‑1 (MQ=255) ttGTCTGAACCTTGGATTTTTTGAAAAATGAAGCACTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac < 3:142611/138‑1 (MQ=255) ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca > 6:148027/1‑137 (MQ=255) tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGGTTTTGGATGGCGGTTCACATTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCt > 2:14323/1‑139 (MQ=255) ttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCat < 5:148027/138‑1 (MQ=255) | GGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT > BS168_reference_genome/1474768‑1475024 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |