Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,481,481 T→C 100% intergenic (+30/‑66) ykzU → / → ykuH conserved hypothetical protein/conserved protein of unknown function

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,481,4810TC100.0% 121.7 / NA 34intergenic (+30/‑66)ykzU/ykuHconserved hypothetical protein/conserved protein of unknown function
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/17);  total (17/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCCTTTAAACGTGCTTTTGATGTTGAGGATTATAGTGAAGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGTCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGTATTATTTTTAAGCAGTTTAGTTATCAT  >  BS168_reference_genome/1481346‑1481602
                                                                                                                                       |                                                                                                                         
aGCCTTTAAACGTGCTTTTGATGTGGAGGATTATAGTGAAGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACCAGCGAACGTTAAAACTGGCCg                                                                                                                         <  1:167688/138‑1 (MQ=255)
      tAAACGTGCTTTTGATGTTGAGGATTATAGTGAAGAGATTGGAAAAATAACAGCACAATCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGc                                                                                                                    <  7:139499/137‑1 (MQ=255)
                 ttgatgttgatgATTATACAGAAGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGAAATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTAtttt                                                                                                        <  3:125684/138‑1 (MQ=255)
                                 taGTGAAGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTGATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGa                                                                                        >  8:123861/1‑138 (MQ=255)
                                      aaGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAt                                                                                  >  3:23274/1‑139 (MQ=255)
                                      aaGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAt                                                                                  >  7:85765/1‑139 (MQ=255)
                                      aaGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAt                                                                                  >  6:112187/1‑139 (MQ=255)
                                         agaTTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAtttt                                                                               >  3:125588/1‑139 (MQ=255)
                                          gaTTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAttttt                                                                              <  8:85765/139‑1 (MQ=255)
                                           aatGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAAttttt                                                                              >  5:81181/3‑138 (MQ=255)
                                           aTTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTa                                                                             <  6:876/139‑1 (MQ=255)
                                            ttGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTaa                                                                            <  8:17311/139‑1 (MQ=255)
                                            ttGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTaa                                                                            <  6:51606/139‑1 (MQ=255)
                                            ttGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTa                                                                             <  4:110204/138‑1 (MQ=255)
                                                     tAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTTAATTTATGATATAATTTTTAAAGATTAAgg                                                                   >  1:189538/1‑139 (MQ=255)
                                                     tAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAgg                                                                   >  1:192442/1‑139 (MQ=255)
                                                     tAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAg                                                                    >  6:193316/1‑138 (MQ=255)
                                                       aCAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGa                                                                  <  5:112187/138‑1 (MQ=255)
                                                          gCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGa                                                              <  4:27178/139‑1 (MQ=255)
                                                                          aTGTTATCTAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCGTGTTTTTTTTTCTATTTAAATTTATTATATAATTTTTAAACATTAAAGAGTTATTCAATGAAGAAAATg                                              >  6:17250/1‑139 (MQ=255)
                                                                          aTGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAACCTGGCCGTTCTCTAGTTTTATTTTCTAATTTAATTTATGATATAATTTTTTAAGATTATGGAGTGATTCATTGAAGAAATTg                                              >  7:189462/1‑139 (MQ=255)
                                                                          aTGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTTAAGATTAAGGAGTGATTCATTGAAGAAATTg                                              >  6:119677/1‑139 (MQ=255)
                                                                            gTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCTAACGTTAAAACTGGCATTTCGCTAGTTTTATTTTCTATTTAAATTTATGATATAATTATTAAAGATTAAGGAGTGATTCATTGAAGAAAATGt                                             >  7:164820/1‑138 (MQ=255)
                                                                              tATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTa                                           >  5:19873/1‑138 (MQ=255)
                                                                                  gagaAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTaaaaaa                                      <  5:193316/139‑1 (MQ=255)
                                                                                     aaaaaaaTGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTATATTTAAATTTATGATTTAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAagt                                    >  4:58296/1‑138 (MQ=255)
                                                                                       aaaaaTGATATCTGACTAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAagttag                                 <  6:9002/139‑1 (MQ=255)
                                                                                       aaaaaTGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGTTTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAagttag                                 <  6:81181/139‑1 (MQ=255)
                                                                                       aaaaaTGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAagttag                                 <  6:9003/139‑1 (MQ=255)
                                                                                       aaaaaTGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAagttag                                 <  4:137208/139‑1 (MQ=255)
                                                                                              atatCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGtat                           <  6:19873/138‑1 (MQ=255)
                                                                                                          aCTGCAAAACAAGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGTATTATTTTTAAGCAg              >  4:119224/1‑139 (MQ=255)
                                                                                                                    aaGCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGTATTATTTTTAAGCAGTTTAGTTatc    <  4:125588/139‑1 (MQ=255)
                                                                                                                      gCGAACGTTAAAACTGGCCGTTCGCTTGTTTTATTTTCTATTTAAATTTTTGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGTATTATTTTTATGCAGTTTAGTTatcct  >  6:50390/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                         
AGCCTTTAAACGTGCTTTTGATGTTGAGGATTATAGTGAAGAGATTGGAAAAATAACAGCACAAGCATTTAGAGATGTTATCGAGAAAAAAATGATATCTGAATAAACTGCAAAACAAGCGAACGTTAAAACTGGTCGTTCGCTTGTTTTATTTTCTATTTAAATTTATGATATAATTTTTAAAGATTAAGGAGTGATTCATTGAAGAAATTGTTAAAAAAGTTAGTTGTATTATTTTTAAGCAGTTTAGTTATCAT  >  BS168_reference_genome/1481346‑1481602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑