| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,690,759 | G→A | 100% | R214H (CGT→CAT) | codY → | transcriptional regulator, GTP and BCAA‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,690,759 | 0 | G | A | 97.6% | 126.9 / ‑4.7 | 40 | R214H (CGT→CAT) | codY | transcriptional regulator, GTP and BCAA‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (16/23); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAA > BS168_reference_genome/1690630‑1690880 | tCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCTTGTCGGCATTACCCATTCTGTTAt > 4:87539/1‑139 (MQ=255) gTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATtg > 1:20706/1‑139 (MQ=255) cAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATtgtg > 8:14152/1‑138 (MQ=255) cTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACt < 4:137749/139‑1 (MQ=255) tCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCa < 6:73890/138‑1 (MQ=255) aGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGa < 6:170411/138‑1 (MQ=255) tCTTACAGTGAGCTTGAATCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGga < 3:13894/137‑1 (MQ=255) tCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGga < 2:171771/137‑1 (MQ=255) cAGTTAGCTTTAAGCAATTGAGCACTTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAgcgc < 2:194757/138‑1 (MQ=255) aGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCg < 2:20706/139‑1 (MQ=255) tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt > 4:110125/1‑139 (MQ=255) tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt > 2:139221/1‑139 (MQ=255) tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACACAGAAAGCTGGAGAGCGCCGgt > 7:154322/1‑139 (MQ=255) tGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGAATTAACAATTCTGTTATTCTGAACGCACTCAGAAAGCTGGAGAGCGCCGgt > 1:194757/1‑139 (MQ=255) aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTg > 8:65523/1‑125 (MQ=255) aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTg < 7:65523/125‑1 (MQ=255) aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGCGCGCCGgtgt > 7:99405/1‑139 (MQ=255) aGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGgtgt > 6:204111/1‑139 (MQ=255) cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTa > 3:165518/1‑139 (MQ=255) cTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTa > 8:192967/1‑139 (MQ=255) gAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc < 2:131927/138‑1 (MQ=255) cAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTc < 5:204111/139‑1 (MQ=255) ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGtt > 5:160472/1‑89 (MQ=255) ttGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGtt < 6:160472/89‑1 (MQ=255) cacaTTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCAt < 1:18577/139‑1 (MQ=255) aggagTTTGACGGAAATGAAGGTCTTCTTGGTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 4:213203/139‑1 (MQ=255) aggagCTTGACTGCAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCGGACCGTGTCGGCATAACCCATTCTGTTATTCTGCACGCACTCCGACAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 4:165518/139‑1 (MQ=255) aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAACTTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 2:157813/139‑1 (MQ=255) aggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGGCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaa < 7:14152/139‑1 (MQ=255) ggagCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGaaa < 8:208717/139‑1 (MQ=255) agCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAgg < 1:161510/139‑1 (MQ=255) cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCTTTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTa > 4:154328/1‑139 (MQ=255) cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGc < 7:112980/80‑1 (MQ=255) cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGc > 8:112980/1‑80 (MQ=255) cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc < 7:172050/111‑1 (MQ=255) cTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATc > 8:172050/1‑111 (MQ=255) aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCa < 7:192967/139‑1 (MQ=255) tgttgCACGTAAAATGCCTCACCGAGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCAGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAaacaac < 2:110999/139‑1 (MQ=255) aaaTTGCTGACCGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTaa < 8:83009/138‑1 (MQ=255) ccGTGTCGGCATTACCCATTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaa < 3:87539/139‑1 (MQ=255) | TCGTACAAATGGCTATCAGCTCGCTTTCTTACAGTGAGCTTGAAGCAATTGAGCACATTTTTGAGGAGCTTGACGGAAATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAA > BS168_reference_genome/1690630‑1690880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |