Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 1,815,227 T→C 9.3% V2436A (GTT→GCT)  pksL → polyketide synthase of type I (bacillaene synthesis)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,815,2270TC 9.3% 119.0 / 4.7 43V2436A (GTT→GCT) pksLpolyketide synthase of type I (bacillaene synthesis)
Reads supporting (aligned to +/- strand):  ref base T (20/19);  new base C (2/2);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

AGACTTCAGAAACGCGCGGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGCAACAAAGAAGCCATTGCTGCTTTTGCTGCTGAT  >  BS168_reference_genome/1815097‑1815354
                                                                                                                                  |                                                                                                                               
aGACTTCAGAAACGCGCGGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGaaa                                                                                                                         <  7:222978/139‑1 (MQ=255)
        gAAACGCGCGGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCaa                                                                                                                 <  8:22723/139‑1 (MQ=255)
         aaaGGCGCGGAGCGATTGCTGCATGCCATTGGTTAACAAACTTATGTTTAAGCATATTTACACCGAATTGCCTATACGGTGCATGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAag                                                                                                                <  2:187324/139‑1 (MQ=255)
              cgcgGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGCAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGACACGATGCAAGAGCtt                                                                                                           >  8:21403/1‑139 (MQ=255)
              cgcgGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAACCGATGCAAGAGCtt                                                                                                           >  6:4157/1‑139 (MQ=255)
              cgcgGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCtt                                                                                                           >  2:31016/1‑139 (MQ=255)
                     cGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGaaa                                                                                                    <  3:180007/139‑1 (MQ=255)
                             gcATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTAt                                                                                            <  6:31402/139‑1 (MQ=255)
                             gcATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTAt                                                                                            >  5:201634/1‑139 (MQ=255)
                              cATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTAt                                                                                            <  3:87133/138‑1 (MQ=255)
                                tGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGaa                                                                                         >  8:36911/1‑139 (MQ=255)
                                     ttCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGa                                                                                                                           >  5:157249/1‑100 (MQ=255)
                                     ttCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGa                                                                                                                           <  6:157249/100‑1 (MQ=255)
                                           aacaaacaTATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCg                                                                               >  5:171112/1‑138 (MQ=255)
                                           aacaaacaTATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCg                                                                               >  5:51144/1‑138 (MQ=255)
                                                          aaGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTg                                                                >  3:184702/1‑138 (MQ=255)
                                                           aGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              <  6:171112/139‑1 (MQ=255)
                                                           aGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              <  5:45836/139‑1 (MQ=255)
                                                            gCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGaaa                                                                                                                         <  7:63450/79‑1 (MQ=255)
                                                            gCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGaaa                                                                                                                         >  8:63450/1‑79 (MQ=255)
                                                                     cacCGAATTGCGTATACTCTGCAAGTAGGCCTCGAAGAGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTAt                                                     <  8:81574/138‑1 (MQ=255)
                                                                     cacCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTAt                                                     <  7:36911/138‑1 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAAGGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              >  2:159001/1‑124 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              <  1:159001/124‑1 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGa                                               >  1:7037/1‑139 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGa                                               >  6:129762/1‑139 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGCTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCgg                                                                              >  8:104635/1‑108 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGCTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCgg                                                                              <  7:101668/108‑1 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGCTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCgg                                                                              <  7:104635/108‑1 (MQ=255)
                                                                          aaTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGCTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCgg                                                                              >  8:101668/1‑108 (MQ=255)
                                                                           aTTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGAc                                              <  7:139149/139‑1 (MQ=255)
                                                                                        tGCAAGTCGGCCGGGAAGGGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGcaa                                 >  4:139872/1‑139 (MQ=255)
                                                                                         gCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              <  8:118074/109‑1 (MQ=255)
                                                                                         gCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGaa                                                              >  7:118074/1‑109 (MQ=255)
                                                                                         gCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGcaa                                 >  7:180462/1‑138 (MQ=255)
                                                                                         gCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGcaa                                 >  6:88852/1‑138 (MQ=255)
                                                                                          cAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGcaac                                >  4:188995/1‑138 (MQ=255)
                                                                                          cAAGTCGGCCGGGAAGCGAAGAAAGAACGGCTGGCGTTCGTTGCTGAAACGATGCAAGAGCTTGAAGAAACGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTATATCGCGGACAAGTAAAGAGcaac                                >  5:223999/1‑138 (MQ=255)
                                                                                              tCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGCAACAaaga                           <  5:88852/139‑1 (MQ=255)
                                                                                                           gATGAAAGAACGGCTGGCGTTCGTTGAGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGaa                                                                       <  5:128908/82‑1 (MQ=255)
                                                                                                           gATGAAAGAACGGCTGGCGTTCGTTGAGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGaa                                                                       >  6:128908/1‑82 (MQ=255)
                                                                                                             tGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGCAACAAAGAAGCCATTGCTGCttt            <  6:51144/139‑1 (MQ=255)
                                                                                                                       gCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGCAACAAAGAAGCCATTGCTGCTTTTGCTGCtgat  <  6:87825/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                               
AGACTTCAGAAACGCGCGGAGCGATTGCTGCATGCCATTCGTGAACAAACATATGTTGAAGCAGATTTACACCGAATTGCCTATACGCTGCAAGTCGGCCGGGAAGCGATGAAAGAACGGCTGGCGTTCGTTGCGGAAACGATGCAAGAGCTTGAAGAAAAGCTGTATGAATGTATCTCCGGAACAGAAAATCGTGAATATGTCTATCGCGGACAAGTAAAGAGCAACAAAGAAGCCATTGCTGCTTTTGCTGCTGAT  >  BS168_reference_genome/1815097‑1815354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑