| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 2,271,523 | A→C | 100% | D45E (GAT→GAG) | uvrX ← | lesion bypass phage DNA polymerase; phage SPbeta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 2,271,523 | 0 | A | C | 100.0% | 70.4 / NA | 24 | D45E (GAT→GAG) | uvrX | lesion bypass phage DNA polymerase; phage SPbeta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/15); total (9/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCA > BS168_reference_genome/2271393‑2271656 | aaaGCTTCTTTTGTGGATTTACAATGTGTATCCTTTGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTAt < 5:222536/138‑1 (MQ=11)aaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTAtt < 4:91023/139‑1 (MQ=12)aaaGCTTCATTTGGGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCAAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTAtt < 2:114941/139‑1 (MQ=11) agagactgGATTTACAATGTGTATCCTTGGATCTTAAGGTATCTCAAATAGTCTCGATCGTGTTTTGATTCAAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCGCTac < 6:54597/132‑1 (MQ=11) aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCa > 6:123075/1‑132 (MQ=255) aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCa < 5:123075/132‑1 (MQ=255) cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCTATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGATATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc < 3:188781/138‑1 (MQ=11) tgtttccTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTATGTGCTGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa < 3:96083/133‑1 (MQ=11) tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa < 2:25911/138‑1 (MQ=255) gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATATTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCACTACAACAGCAAGATAGCATGTTAAAGGGTTAAg > 5:168063/1‑139 (MQ=255) cAAATAGTCTCGATCCTGTTTTGATTCAAAAATCTTTTTTAAGTGCATGAGATGCAGATAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCACCATTGTTACAGc < 6:168063/139‑1 (MQ=14) tctcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATa > 8:198045/1‑137 (MQ=255) gATCCTGTTTTGATTCCAAAATGTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAg < 5:74518/139‑1 (MQ=255) tAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACaaa < 7:198045/138‑1 (MQ=255) gaTGCAGCTAATAGAACACTTCACTGTCTCTCCGTCTTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACTGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTa < 5:11524/138‑1 (MQ=14) gCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTg > 1:14919/1‑139 (MQ=255) tAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca > 4:66851/1‑117 (MQ=255) tAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca < 3:66851/117‑1 (MQ=255) cactTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGACGCATAAAAGGATTGCATGTCGAGACAAAGTATATTCTTACGTGGAAATAGTGAGtt > 4:127434/1‑138 (MQ=255) ttCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGc < 7:31318/56‑1 (MQ=255) ttCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGc > 8:31318/1‑56 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca < 7:146788/104‑1 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca > 8:146788/1‑104 (MQ=255) cccTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTaatcaa > 6:131318/1‑139 (MQ=255) tctctcCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAatcaatca < 2:14919/137‑1 (MQ=255) tctctcCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAatcaatca < 6:52588/137‑1 (MQ=255) | AAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCTTACGTGGAAATTGTGAGTAATCAATCA > BS168_reference_genome/2271393‑2271656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |