| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 4,153,115 | G→T | 100% | L192I (CTT→ATT) | walK ← | two‑component sensor histidine kinase [YycG] |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,153,115 | 0 | G | T | 92.1% | 64.2 / 0.4 | 26 | L192I (CTT→ATT) | walK | two‑component sensor histidine kinase [YycG] |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (14/9); total (16/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCGG > BS168_reference_genome/4152985‑4153243 | tcatGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCCAAACAATTGCCAAAc > 8:61170/1‑139 (MQ=255) cTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGa < 3:97123/138‑1 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGcc > 4:124745/1‑139 (MQ=255) tttAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAATGCCAAACCGGTGCCGGACGc > 6:280/1‑138 (MQ=255) ccTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGGCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTAt < 2:184973/139‑1 (MQ=255) tCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCgt > 1:72149/1‑139 (MQ=255) gagaAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 2:72149/139‑1 (MQ=255) gCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTTCCGGACGCCAGTATAGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCAt > 6:202933/1‑139 (MQ=255) gCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGGCTTGATTTGATTAAAGACATCTTCCa > 4:57049/1‑138 (MQ=255) gCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAG‑AAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGCTCGTCTTCATTTGATTAAATCCATCTTCCATACTcgcg > 8:215052/1‑139 (MQ=255) atatCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGa < 3:77743/139‑1 (MQ=255) tCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa < 8:77680/97‑1 (MQ=255) tCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGa > 7:77680/1‑97 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATcgcg > 3:47762/1‑139 (MQ=255) gtgggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATcgcg > 2:49079/1‑139 (MQ=255) ggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGAt < 2:154631/84‑1 (MQ=255) ggtAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGAt > 1:154631/1‑84 (MQ=255) cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGTCGCCAGTTTGGTGTTGAGCGTGGTGGTTTGATTTAAGCCCTCTTCCATACGCGCGCCACCGTAGAGCGCGCCCCCAcccgcgg > 2:125503/1‑135 (MQ=255) cTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGCCAACGTAGCTCGCGCCCACAACctcgt > 7:86751/1‑137 (MQ=255) ccAGAAAAATACCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGtt < 4:47762/139‑1 (MQ=255) aCCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg < 7:11380/40‑1 (MQ=255) aCCGAGAAGAGCCGTCAAAACAATTGCCAAACCTGTGCCg > 8:11380/1‑40 (MQ=255) agaagaGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACg > 4:225409/1‑99 (MQ=255) agaagaGCCGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACg < 3:225409/99‑1 (MQ=255) cGTCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTcgcg < 7:61170/139‑1 (MQ=255) tCAAAACAATTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCgg > 7:66331/1‑138 (MQ=255) | TCATGTCCATACTTTTTAACCTTCCTCGAGAAATTCCCTTTCGCAAGCTCCATCGCCTGCTTTCGCATATCTGAAAGCGGGTGGGTAATGGTTCTTGCCAGAAAAATACCGAGAAGAGCCGTCAAAACAAGTGCCAAACCTGTGCCGGACGCCAGTATCGTGTTGATCGTCTTCATTTGATTAAAGACATCTTCCATACTCGCGACAACGTAGATCGCGCCCACAACCTCTTGGTTTTCAGTCATGACGGGCTTCGCGG > BS168_reference_genome/4152985‑4153243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |