BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	116	T→A	5.1%	intergenic (–/‑2862)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	116	0	T	A	5.1%	17677.7 / 144.4	6427	intergenic (–/‑2862)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base T (2551/3450); new base A (9/413); total (2563/3864)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 6438 total aligned reads displayed.

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTG  >  pSIJ663_pHT315_araE_xy/1‑252

ccctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                        >  2:29120‑M1/22‑139 (MQ=255)

ccctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                        <  7:41113‑M1/118‑1 (MQ=255)

ccctgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                        <  5:134120‑M1/118‑1 (MQ=255)

   tgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                        <  6:59454‑M1/118‑1 (MQ=255)

   tgataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTaa                                                                                                                                        <  8:10749‑M1/118‑1 (MQ=255)

     ataaatgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGaa                                                                                                                                   <  7:208507‑M1/123‑1 (MQ=255)

          tgcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGATCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAacgta                                                                                                                               <  4:120148‑M1/127‑3 (MQ=255)

           gcttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaaa                                                                                                                             <  1:158790‑M1/129‑1 (MQ=255)

            cttcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAAt                                                                                                                            <  7:139221‑M1/130‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACTTAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                          <  1:219497‑M1/132‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGaa                                                                                                                              <  7:194746‑M1/128‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                          <  7:67702‑M1/132‑1 (MQ=255)

               caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                          <  7:152957‑M1/132‑1 (MQ=255)

                    aTCCATCCTCCAACGTTGCAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTCATTAAAAACAAACATAGCGGAAAAATTAGCAGTAACTAAATAGACCGGAAATGAAAAAAg                                                                                                                    >  8:54661‑M1/2‑139 (MQ=255)

                    aTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAg                                                                                                                    <  3:2686‑M1/138‑1 (MQ=255)

                     tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                   >  5:15000/1‑139 (MQ=255)

                     tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                   >  1:187039/1‑139 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                 >  8:23518/1‑139 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                 <  3:13734/139‑1 (MQ=255)

                       cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTATTGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAAAAGAAAAATAGCGGTAAAATTACCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                 <  3:54934/139‑1 (MQ=255)

                       cATCCTCAAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGTAAATGAAAAACGCCa                                                                                                                 <  5:99825/139‑1 (MQ=255)

                       aaTCCTCCAAAGTTGGTGAGTGAGTTTTATGTCGCAAATATTAATGTTTGTGGTGAACATTATCAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAAGGTAAATGAAAAAAGCCa                                                                                                                 <  2:116925/138‑1 (MQ=255)

                       aaTCCTCCAAAGTTGGATAGTGTGTTTTATGTCGAAAATATTAATGTTTCTGGTTAAAATTAACAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                 <  3:70123/138‑1 (MQ=255)

                         tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                               >  5:5818/1‑139 (MQ=255)

                         tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                               <  5:103150/139‑1 (MQ=255)

                         tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTAAGCAAATTTTCGTACATTAAATAGAAACATAGCGGAAAAATAAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCAct                                                                                                               >  6:134283/1‑139 (MQ=255)

                          cctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                              >  5:84277/1‑139 (MQ=255)

                             cagaaGTTGGATATTGTGTTTTATTTCGAAAATATTAATGTTTCTGGTGAAACTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGTTAAAATTACCATTAAATTAATAGAACGTAAATGAAAAAAGCCACtctc                                                                                                            <  8:157111/135‑1 (MQ=255)

                              cAAATTTGGCTAGTGATTTTTATGGCGCAAATATTAATGTTTCTGGTCAACCTTATAAAATTTTCGTTTATTTAATAGAAACATACCGGAAAAATTCGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCat                                                                                                          <  5:27194/139‑1 (MQ=255)

                                     ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                   >  1:133501/1‑139 (MQ=255)

                                     ggAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGTTGAACCTTATCAAATTTTCGTTGATTAAATAGAAAAATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTa                                                                                                    <  7:164857/138‑1 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACTGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                  >  6:205959/1‑139 (MQ=255)

                                      gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                  <  3:82801/139‑1 (MQ=255)

                                           tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                             >  3:121373/1‑139 (MQ=255)

                                            gagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                            >  5:4413/1‑139 (MQ=255)

                                            gagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGAGTTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTgtatac                                                                                            >  7:1269/1‑134 (MQ=255)

                                             tgtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAAAAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  7:25465/138‑1 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACTGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                            >  6:170694/1‑138 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  4:171082/139‑1 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  4:188392/139‑1 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  1:62417/139‑1 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           >  8:85081/1‑139 (MQ=255)

                                             agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                            >  1:146205/1‑138 (MQ=255)

                                             agtTTTATGGCGAAAATATTAATGTTTCTGGTGAACCTTATCAATTTTGCGTTGATTTAAAAGAAACATAGCGTAAAAATTACCAGTAACTAAATACACGGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  2:81511/139‑1 (MQ=255)

                                              gtgttATGTCTCAAATATTATGGTTTCTGTTTAAAATAAAAAAATTTTCGTTTAATAAATATAAAATTAGCGATAAATTAAGATCTCAATTAGAAGATCTAAAATCAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                           <  4:39745/135‑1 (MQ=255)

                                                     gTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCtt                                                                                     >  2:134587/1‑137 (MQ=255)

                                                      gctaaaaTATTAATGTTTCTTGTGAAACTTATAAAATTTTTGTTTTTTTAATACAAACATAGCTTTAAAATTATCAGTAAATTAATATAACCTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTa                                                                                   <  2:61905/134‑1 (MQ=255)

                                                       cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCTGTAAAATTAGCAGTATCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGATATTGGCTACCAACCTTTAGc                                                                                 >  7:79519/1‑139 (MQ=255)

                                                       cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                 >  7:56075/1‑139 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                 >  8:204867/1‑137 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                 >  3:18563/1‑137 (MQ=255)

                                                         cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                 >  5:70135/1‑137 (MQ=255)

                                                            ttattatTGTTTCTGGTCAACCTTATCAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTAGAATTAACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  8:18748/138‑1 (MQ=255)

                                                            atatTAATTTTTCTTGTGAACCTTATCAAATTTTCGTTGTTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  1:173518/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  2:168743/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  5:182616/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  6:95890/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAAATTAATACAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  1:10003/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  3:154415/139‑1 (MQ=255)

                                                            atatTAATGTTTCTGGTGAAACTATTAAAATTTTCGTTTATTTAATAAAAACAAAGCTTTAAAATTAGCAGAAAATAAATAACACGTAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  4:100781/139‑1 (MQ=255)

                                                             tatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAAt                                                                           <  7:180630/139‑1 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                    <  8:70057/136‑1 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                 >  1:76700/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                 >  1:92850/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGAGAGAATGACTTAATcc                                                                 >  3:3898/1‑139 (MQ=255)

                                                                       tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                 >  7:94461/1‑139 (MQ=255)

                                                                       tCTGGGCAACCTTATCAAATTTTCGTTGATTTAATTTAAACATAGCGTTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                  <  8:40104/138‑1 (MQ=255)

                                                                       gcTGGAGAACCTTAACAAATTTTCTTTTATTAAATCCAAAAATAGCGGTAAAATAAGCAGTAACTTAATCCACCGCAAATCAACAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGATTGACTTAATc                                                                  <  6:115334/137‑1 (MQ=255)

                                                                        cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGAAACTCTCATATGCTATTGGCTACCAACATTTAGCTAGAATGACTTAATCCt                                                                >  3:103977/1‑139 (MQ=255)

                                                                           tttaaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                             <  2:151475/136‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATCGCTGTAATATTAGCAGAAACTTAATAGAACGTAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                             <  5:118/139‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                             <  3:215811/139‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGTTGATTAAATACAAACATAGCGGAAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                             <  3:199266/139‑1 (MQ=255)

                                                                           gTGAACCTTATCAAATTTTCGATGATTTAATAGAAACATAGCGGTAAAATTCGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                             <  6:42791/139‑1 (MQ=255)

                                                                              aaCATTATCAATTTATGGTTGTTTTAAAAGAAAGATACCGTTTAAATTACCATTAAGTAAAAAGACCTGAATTTAAAAACGCCACTCTCATATGCTATTTGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                          <  2:207147/139‑1 (MQ=255)

                                                                               aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                          <  1:12008/138‑1 (MQ=255)

                                                                                   tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                     >  7:109485/1‑139 (MQ=255)

                                                                                   tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                     >  1:201953/1‑139 (MQ=255)

                                                                                     taaaaTTTTCTTGTATTTAATAGAAAAATAGCGGTAAAATAACCCTTAAATTAATCTAACGTAATTTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                    <  4:138614/136‑1 (MQ=255)

                                                                                     tCAAATTTTCGTTTATTAAATACAAACATAGCGGTAAAATAAGCAGTAACTTAATACAACGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACa                                                   <  6:77331/139‑1 (MQ=255)

                                                                                     tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                     >  4:15436/1‑137 (MQ=255)

                                                                                     tCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                    <  8:141479/138‑1 (MQ=255)

                                                                                            ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                             <  4:205961/138‑1 (MQ=255)

                                                                                             tCGTTGATATAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                            >  5:211339/1‑138 (MQ=255)

                                                                                              cGTTGATTTAATATAAAAATAGCGTTTAAATTACCATTAACTTAAAAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCg                                           <  4:54486/138‑1 (MQ=255)

                                                                                                   aTTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                            <  8:1254/100‑1 (MQ=255)

                                                                                                              aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGgctgtc                          >  1:36805/1‑135 (MQ=255)

                                                                                                                aacgCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                        <  5:166340/136‑1 (MQ=255)

                                                                                                                  aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTg                                                               <  6:154397/98‑1 (MQ=255)

                                                                                                                  aGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                      >  2:4607/1‑139 (MQ=255)

                                                                                                                      ttAAAATTCGCATTAAATTAATTCAACGCAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                  <  6:19085/138‑1 (MQ=255)

                                                                                                                       tAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                  >  1:50039/1‑138 (MQ=255)

                                                                                                                        aaaaTTAGCAGTAACTAAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                 <  2:187039/138‑1 (MQ=255)

                                                                                                                              aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt          >  4:86767/1‑139 (MQ=255)

                                                                                                                               gCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAtt         >  4:69180/1‑139 (MQ=255)

                                                                                                                                cAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGTGCCAACTTCAAATAAGTTAttt        >  5:197039/1‑139 (MQ=255)

                                                                                                                                   tAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGcc     <  8:172956/139‑1 (MQ=255)

                                                                                                                                     aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt   >  3:171283/1‑139 (MQ=255)

                                                                                                                                     aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt   >  4:224349/1‑139 (MQ=255)

                                                                                                                                     aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt   >  6:223202/1‑139 (MQ=255)

‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTG  >  pSIJ663_pHT315_araE_xy/1‑252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`