BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	2,207	C→T	8.3%	intergenic (–/‑771)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	2,207	0	C	T	8.3%	29303.0 / inf	9252	intergenic (–/‑771)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base C (3384/5041); new base T (305/467); total (3727/5525)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-04

Only 100 of 9398 total aligned reads displayed.

TGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  pSIJ663_pHT315_araE_xy/2068‑2344

    ttATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCAt                                                                                                                                       >  4:81194/1‑139 (MQ=255)

     tataAAACCCGCAAACATTTTAACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTCTGGAAGTTTAGCAACAACAGTAGTTTTATTTACAATC‑TTATTTTTATTAGCCGTCAAATTTCTCCCTCa                                                                                                                                        >  8:69198/1‑138 (MQ=255)

          aTCCTGGAAACATTTTTACATAGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAAGAGGTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  <  2:85753/139‑1 (MQ=255)

          aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  >  1:81344/1‑139 (MQ=255)

          aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  <  3:184215/139‑1 (MQ=255)

          aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  <  8:53898/139‑1 (MQ=255)

          aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  <  4:189038/139‑1 (MQ=255)

          aTCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  <  6:197154/139‑1 (MQ=255)

             cGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGtctc                                                                                                                               >  5:203818/1‑139 (MQ=255)

               ggAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCtt                                                                                                                             >  8:37974/1‑138 (MQ=255)

                 aaaCATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTta                                                                                                                           <  2:149833/139‑1 (MQ=255)

                    cATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTatat                                                                                                                        <  6:48667/139‑1 (MQ=255)

                      tttttACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCT                                                                                                                      <  4:109145‑M1/139‑1 (MQ=255)

                              tCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGGCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCtttgtttta                                                                                                              <  1:217093/139‑1 (MQ=255)

                                 ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCa                                                                                                           >  7:175452/1‑139 (MQ=255)

                                 ttAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCa                                                                                                           >  1:67173/1‑138 (MQ=255)

                                      aGTGCAAGTCAAGTTATATGTATCTATCAAGCTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTATTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     <  8:190241/139‑1 (MQ=255)

                                       gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAgg                                                                                                     <  3:44529/139‑1 (MQ=255)

                                       gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAgg                                                                                                     <  2:222/139‑1 (MQ=255)

                                       gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAgg                                                                                                     <  2:168116/139‑1 (MQ=255)

                                       gTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     <  5:161409/139‑1 (MQ=255)

                                        tGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGa                                                                                                    <  4:22404/139‑1 (MQ=255)

                                         gCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATTTATTTATTTTATCATAAAGTAg                                                                                                   >  3:7499/1‑139 (MQ=255)

                                          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGt                                                                                                  >  7:83716/1‑139 (MQ=255)

                                          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCTTAAAGGAGTa                                                                                                 >  5:6934/1‑139 (MQ=255)

                                          caagtcaagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTGTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGat                                                                                                   >  7:37565/1‑137 (MQ=255)

                                               caagtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTGTTTATATCTTTATTTTGTCATAAAGGAGTATTTg                                                                                             >  2:71295/1‑139 (MQ=255)

                                                  gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTgttt                                                                                                                <  1:151194‑M1/117‑5 (MQ=255)

                                                  gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTAGATCTTTGTTTTATCAGAAAGGATTATTTGAAc                                                                                          >  6:11752/1‑139 (MQ=255)

                                                  gtTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGt                                                                                                                                  >  4:36631/1‑98 (MQ=255)

                                                     atatGTCTCTATTATGATGTGTGGAAGTTTTGCCACATCAGTTGGTTTATTTACAATC‑TTTTTTTTCTTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATCTCTTTATTTTCTCGTAAAGGAGTATTTGAACCg                                                                                        <  1:97795/138‑1 (MQ=255)

                                                     atatGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCg                                                                                        <  1:166839/138‑1 (MQ=255)

                                                       atGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAgg                                                                                                     <  1:200100/122‑1 (MQ=255)

                                                          tATCTATATTGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  8:163378/138‑1 (MQ=255)

                                                          tATCTATAATGATTTGTGGCAGTTATGCCACAACAGTCTGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  7:223435/138‑1 (MQ=255)

                                                          tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  2:56638/138‑1 (MQ=255)

                                                          tATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  3:91895/138‑1 (MQ=255)

                                                           aTCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGTAGTATTTGAACCGTCGCGCgg                                                                                >  5:223065/1‑139 (MQ=255)

                                                            tCTATAATGATTTGTGGGAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTAttt                                                                                              >  5:189962/1‑125 (MQ=255)

                                                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATAATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  5:498/1‑139 (MQ=255)

                                                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATaa                                                                                                        >  7:128876/1‑115 (MQ=255)

                                                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  1:18483/1‑139 (MQ=255)

                                                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  1:132869/1‑138 (MQ=255)

                                                            tCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  8:205068/1‑138 (MQ=255)

                                                             cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCAtct                                                                                                                                     <  4:94574/85‑1 (MQ=255)

                                                             cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTGTCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  4:139678/1‑138 (MQ=255)

                                                             cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCggg                                                                               >  6:150154/1‑139 (MQ=255)

                                                             cTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCgg                                                                                >  2:1147/1‑138 (MQ=255)

                                                              tataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  2:37186/139‑1 (MQ=255)

                                                              tataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTAGTCTCTTTATATCTTTGTTTTATAATAAAGGAGTTTTTGAACCGTCGCGCGGGa                                                                              <  7:69198/139‑1 (MQ=255)

                                                               ataATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  8:17697/139‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTTGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  1:50827/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTTGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGATCCGTcg                                                                                     <  3:101218/130‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATCTTTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  4:154540/139‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTTCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGGACCGTCGCGCGGGa                                                                              <  3:198725/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  3:167687/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  3:59978/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  5:35961/1‑137 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  3:216472/138‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCg                                                                                        <  3:2777/127‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:117928/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              >  1:116834/1‑137 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  7:146094/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:84836/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  3:150326/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGAc                                                                             <  3:134519/138‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTACATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  8:134685/137‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  1:100035/136‑1 (MQ=255)

                                                                taATGATTTGTGGAAGTTTTGCCAAAACAGTTTGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCCTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  2:185507/137‑1 (MQ=255)

                                                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGttt                                                                       <  7:53046/139‑1 (MQ=255)

                                                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTTTCGTAAAGGAGTATTTGAACCGTcgcg                                                                                   >  4:124645/1‑127 (MQ=255)

                                                                     aTTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTcgcg                                                                                   <  7:113873/127‑1 (MQ=255)

                                                                      tttGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCTCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTa                                                                      >  6:97616/1‑139 (MQ=255)

                                                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCGTAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATg                                                                    >  7:160707/1‑139 (MQ=255)

                                                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATGAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  6:179187/129‑1 (MQ=255)

                                                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGtt                                                                        <  2:204833/135‑1 (MQ=255)

                                                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                     >  7:20579/1‑138 (MQ=255)

                                                                        tgtgGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATAATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTAt                                                                     >  5:111301/1‑138 (MQ=255)

                                                                            gAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAg                                                                <  6:135481/139‑1 (MQ=255)

                                                                                      cacaACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                      <  7:189948/139‑1 (MQ=255)

                                                                                      cacaACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTa                                                      <  4:67047/139‑1 (MQ=255)

                                                                                           caGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTCATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaa                                                 <  3:119052/139‑1 (MQ=255)

                                                                                             gTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGaata                                               <  6:83675/139‑1 (MQ=255)

                                                                                              ttGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATAATTTATTGaataa                                              >  4:172853/1‑139 (MQ=255)

                                                                                                 gTTTATTTACAATC‑‑TTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  5:84597/139‑1 (MQ=255)

                                                                                                  tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  6:213879/139‑1 (MQ=255)

                                                                                                  tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCGTCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  8:148834/139‑1 (MQ=255)

                                                                                                  tttatttaCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  8:222471/139‑1 (MQ=255)

                                                                                                  tttatttaCAATC‑TTGTTTTTATTAGCCGTCAAATTTCTCCCTCCTCTCGTCTCTTTCTATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGa                                          <  5:143511/139‑1 (MQ=255)

                                                                                                  tttaattaAAATC‑GTTTTTTTAATACCCCGCAAAATTCCCCCTCCTCTCCTCTCTTTATATATTTAATTTATCAAAAAGGTCTATTTGCACCGGCCCCCGGGACAGGTTTATTTTACGGAAATTTTATTGAATAATTGa                                          <  6:100555/139‑1 (MQ=255)

                                                                                                        taCAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGa                                                                              <  5:174753/97‑1 (MQ=255)

                                                                                                            aTC‑TTTTTTTTATTAGCCGTCAATTTTCTCCCTCATCTAGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                <  6:6842/139‑1 (MQ=255)

                                                                                                            aTC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTGTTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAgg                                <  8:100502/139‑1 (MQ=255)

                                                                                                              atctttttttATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCATTATATAGTTATTTTATATTAAAGGAGTTTTTGAACCGTCGCGCGGGCCAGGTTTAGGATAGGGATATTTTATTTAATAATTGATGGTATAAgg                                >  4:48979/4‑138 (MQ=255)

                                                                                                                 tttttttATTAGCCGTCAAATTTCTCCCTCATCTCTTCTCTTTATATCTTTATTTTATAGTAAATGAGTATTTGAACCGTCGCGCTGGACAGGTTTATGATAGGGATATTTTATTTAATAATTGATTGTTTaattgaat                            >  2:131185/1‑133 (MQ=255)

                                                                                                                    ttttATTAGCCGTCAAATTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACttt                          <  6:30364/138‑1 (MQ=255)

                                                                                                                      ttattaGCCCTCTAATTTCTCCTTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGTGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCa                        <  1:34146/138‑1 (MQ=255)

                                                                                                                                  aattttCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGt            <  4:206167/135‑1 (MQ=255)

                                                                                                                                  aaaTTTCTCCCTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGt            <  5:127416/138‑1 (MQ=255)

                                                                                                                                           ccTCATCTTGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTAt   <  6:165686/138‑1 (MQ=255)

TGCTTTATAAATCCGGGAAACATTTTTACATCGTTAGAAGTGCAAGTCAAGTTATATGTATCTATAATGATTTGTGGAAGTTTTGCCACAACAGTTGGTTTATTTACAATC‑TTTTTTTTATTAGCCGTCAAATTTCTCCCTCATCTCGTCTCTTTATATCTTTATTTTATCATAAAGGAGTATTTGAACCGTCGCGCGGGACAGGTTTATGATAGGGATATTTTATTGAATAATTGATGGTATAAGGGACTTTCATGCTTGGAAAGTGGGGATTATG  >  pSIJ663_pHT315_araE_xy/2068‑2344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`