BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	10.0%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	10.0%	13295.0 / inf	4658	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (244/3896); new base T (501/17); total (745/3913)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4728 total aligned reads displayed.

TAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3598‑3877

     aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTg                                                                                                                                          <  1:177318/139‑1 (MQ=255)

     aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTT‑AAtttt                                                                                                                                          >  6:4787/1‑138 (MQ=255)

     aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTGTTT‑TAtttt                                                                                                                                          >  4:91086/1‑138 (MQ=255)

      acAGTATCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTgg                                                                                                                                         <  1:94976/138‑1 (MQ=255)

       cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGa                                                                                                                                        <  5:80448/139‑1 (MQ=255)

        aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGa                                                                                                                                        <  2:187374/138‑1 (MQ=255)

        aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGa                                                                                                                                        <  6:78408/138‑1 (MQ=255)

        aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGa                                                                                                                                        <  3:60815/138‑1 (MQ=255)

          tttCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AAtt                                                                                                                                            >  7:48762/1‑132 (MQ=255)

              atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTac                                                                                                                                 <  8:53426/139‑1 (MQ=255)

              atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTa                                                                                                                                  <  4:217406/138‑1 (MQ=255)

              atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTa                                                                                                                                  <  5:207373/138‑1 (MQ=255)

              atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAAGTTa                                                                                                                                  >  6:94443/1‑138 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAGTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  8:204395/139‑1 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  6:29851/139‑1 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  6:13826/139‑1 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  7:16626/139‑1 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  7:213671/139‑1 (MQ=255)

               tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGGACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTaca                                                                                                                                <  8:6468/139‑1 (MQ=255)

                 aatcAATTAATCAAGACACAGTAAAGAATTTAAGTACCATTACTTATGATCGAGTATTGTCTATTTTTAATAGATATCTCTTATTTTACGGGTGGAAATAATTCTATGAGTCGC‑TTTTTTA‑ATTTTGGATAGTTACACg                                                                                                                              <  8:159450/136‑1 (MQ=255)

                 aaGCAATGAAACACGCCAAAGTTAACAAATTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                              <  1:80707/139‑1 (MQ=255)

                 aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                              <  2:42188/139‑1 (MQ=255)

                 aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                              <  8:172610/139‑1 (MQ=255)

                 aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACg                                                                                                                              <  3:31435/139‑1 (MQ=255)

                      aTGTAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                         <  6:110719/139‑1 (MQ=255)

                      aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                         <  2:118469/139‑1 (MQ=255)

                      aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                         <  3:43674/139‑1 (MQ=255)

                      aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                         <  3:208377/139‑1 (MQ=255)

                      aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACt                                                                                                                         <  4:175740/139‑1 (MQ=255)

                         aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTaaa                                                                                                                      <  4:156460/139‑1 (MQ=255)

                         aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTaa                                                                                                                       <  3:86949/138‑1 (MQ=255)

                              cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  1:63146/139‑1 (MQ=255)

                              cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                 <  4:116750/139‑1 (MQ=255)

                               gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTA‑AATTT‑GAAATTTTAACGTTAATAAAGGGAAt                                                                                                                >  8:189119/1‑139 (MQ=255)

                               gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTATAATTTGTAAATTTA‑ACCTTTCTAAAAGTAAt                                                                                                                >  8:3567/1‑139 (MQ=255)

                                     ttataCAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                          <  5:130185/135‑1 (MQ=255)

                                      tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  7:196481/139‑1 (MQ=255)

                                      tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  3:224208/139‑1 (MQ=255)

                                      tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  7:148589/139‑1 (MQ=255)

                                        aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATGTTTTATTTAAAGGGAGGAAATAATTCTATGAGTCGG‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  3:188669/139‑1 (MQ=255)

                                         aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAAGTTTGACGGTACTTAAGGGAATGGAGATAAat                                                                                                      >  8:106975/1‑139 (MQ=255)

                                         aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTAGGAGTCGC‑TTTTTTA‑AAATTGGAAAGTTTCCAGGTGCTAAAGGGAATGtagtttata                                                                                                      >  7:182353/1‑133 (MQ=255)

                                          cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                     <  1:49832/139‑1 (MQ=255)

                                             tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTTATAGTTATATAATATTTAACGGGATGAAATAAATCAATGAATCCCGGTTTTTA‑AATTTTTAAAATTAAAAGTAAATAAAGCTAATGCA‑ATAAattaat                                                                                                  >  2:57873/1‑137 (MQ=255)

                                             tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  3:72241/139‑1 (MQ=255)

                                             tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  6:197179/139‑1 (MQ=255)

                                             tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  1:150802/139‑1 (MQ=255)

                                                aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  7:74985/138‑1 (MQ=255)

                                                aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  5:174701/138‑1 (MQ=255)

                                                  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  5:104240/139‑1 (MQ=255)

                                                  gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  2:97042/139‑1 (MQ=255)

                                                  gTACCATTACTTATGAGCAAGTATTGTCTAATTTTTATAGTTATCTATTATTTTACGGGAGGAAATAATTATATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  2:154534/139‑1 (MQ=255)

                                                     ccATTACTTATGAGCAAGTAATGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTT‑AATTTTTAAATTTAAACCTTAAAAAATGGAATGGATAAAAAATATtatataat                                                                                           >  2:20872/1‑136 (MQ=255)

                                                            ttATGTTCAAGTATTTTCTATTTTTAATAGTTATCTATTTTTTATCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATTAATTATTAGATATACTACTGa                                                                                    <  3:143388/138‑1 (MQ=255)

                                                            ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  4:197884/139‑1 (MQ=255)

                                                            ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  5:50380/139‑1 (MQ=255)

                                                              aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  5:219060/137‑1 (MQ=255)

                                                                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTT‑AATTTGGAAAGTTTCACGTTTCTAAAGGGATTGGAGTTAAATTTTTATATATAATACTGACCGc                                                                                >  1:163377/1‑139 (MQ=255)

                                                                gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑TATTTTGAAATTTTCACGGTAGTAAAGGGTATGGTGATTAATTTTTATATATAATAATGACAGCt                                                                               >  8:140850/1‑139 (MQ=255)

                                                                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTTTATGAGTCGC‑TTTTTTA‑AATATGGAAAGGTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:115211/139‑1 (MQ=255)

                                                                    aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TATTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  7:92083/139‑1 (MQ=255)

                                                                     aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTTGTAAGTTTAACGTTTCTAAAGGGAATGGAGGTTAATTTTTATATATACTAATGACACCTTACa                                                                           >  4:37360/1‑138 (MQ=255)

                                                                     aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTT‑AATTTGGTAATTTAAAAGTTAATAAAAGGAATTGAGATAAATTATTTTATTTACTTCTGACAACTTGCa                                                                           >  7:38038/1‑138 (MQ=255)

                                                                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTA‑AATTTTAAAATTTA‑AAGTTAATAAATTGAATTGAGCAAAATTATTTTATATAATAATTAAAGCTTACaa                                                                          >  1:208884/1‑138 (MQ=255)

                                                                      gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  7:122948/139‑1 (MQ=255)

                                                                       tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  3:173857/139‑1 (MQ=255)

                                                                             cTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                   <  5:191762/138‑1 (MQ=255)

                                                                               aTTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTAACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  1:193217/138‑1 (MQ=255)

                                                                                tttttAATAGTTATCTATTATTTAACGGGTGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                <  4:56569/138‑1 (MQ=255)

                                                                                tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                <  4:136684/138‑1 (MQ=255)

                                                                                  tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  1:222255/138‑1 (MQ=255)

                                                                                            aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTA‑AATTTTGATAGTTTAACGTTTCTAAAGGGAAATGAGATAAATTATTATATATAAAAATGACAGCTTCCAACAAACTAAATAGGTCCCTAgcgc                                                   >  8:219869/1‑139 (MQ=255)

                                                                                                             aggaAATACTTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                  <  5:146177‑M1/139‑4 (MQ=255)

                                                                                                                 aattatTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAATGTAATGGAGATATATTATTTTATATACTACTGAGAGGTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  3:112090‑M1/139‑8 (MQ=255)

                                                                                                                 aataatTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  3:127545‑M1/139‑8 (MQ=255)

                                                                                                                 aataatTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  5:96454‑M1/139‑8 (MQ=255)

                                                                                                                 aataatTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  4:72929‑M1/139‑8 (MQ=255)

                                                                                                                     cttcTATGATGCGC‑TATTTTA‑AATTTGGAAAGTTAAAAGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgtgtacattga                          <  2:172817‑M1/138‑12 (MQ=255)

                                                                                                                           ttagtCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:27709‑M1/137‑18 (MQ=255)

                                                                                                                           tGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTAGTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  4:100662‑M1/139‑18 (MQ=255)

                                                                                                                           tGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  8:122990‑M1/139‑18 (MQ=255)

                                                                                                                           tGAGTAGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACATCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  8:79880‑M1/139‑18 (MQ=255)

                                                                                                                           tGACTCGC‑TTTTTTA‑ACTGTGGCAACTGACACGGTACTAAAGGCAAAGGAGACAAAATATTAGATATACAACAGACAGCGTCCCAGAAGCTAAAGAGGGCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAgg               <  2:76835/139‑1 (MQ=255)

                                                                                                                            gtgtcGC‑TTTTTTA‑AATTTGGAAAGTTACACTTTACTAAAGGGAATTGAGATAAATTATTAGATATACTTCTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                    <  1:100491‑M1/136‑18 (MQ=255)

                                                                                                                            gAGTCGC‑TTTTTTA‑AATTTTGAAAGTTACACGTTACTAAAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGTAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  7:136923‑M1/138‑18 (MQ=255)

                                                                                                                             attcgC‑TTTTTTA‑TATTTGGTAAGTTACACGTTACTAAAGGGAATGTATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagga                   <  1:121595‑M1/136‑19 (MQ=255)

                                                                                                                             aGTCGC‑TTTTTTA‑AATTTGGAAATTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  5:65079‑M1/139‑20 (MQ=255)

                                                                                                                             aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  6:212350‑M1/139‑20 (MQ=255)

                                                                                                                             aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:23918‑M1/139‑20 (MQ=255)

                                                                                                                             aGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:59927‑M1/139‑20 (MQ=255)

                                                                                                                             aGTCGA‑TTTTTTA‑AATTTTTAAATTTACAAGTTACTAAAGTGAATGTATATAAATTATTAGATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACTGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa             <  6:177268/139‑1 (MQ=255)

                                                                                                                              gTCGC‑TTTTTTA‑AATTTTGAAAGTTACAAGTTACTAATGGTTATCGAGATAAATTTTTAGATATACTACTTACAGCTTCCAAGAATATAAAGAGGTCCCTTGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  3:157456‑M1/138‑20 (MQ=255)

                                                                                                                               ttagatttttta‑aattTGGAAAGTTACACGTCACTAAAGGGAATGGAGATAAATTATTAGATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  1:136191‑M1/133‑20 (MQ=255)

                                                                                                                                     tttttA‑AATTTGGAAAGTTACACGTAACTAAAGGTAATGGAGATAAATTATTATATATACTACTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:157740‑M1/138‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  6:62962‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:36730‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  2:138847‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGCAATGTGACAGGTTCCTAATGGGAATGGAGATAAATTATTAGATATACTACTGCCAGCTTCCAAGATGCTTAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  6:159440‑M1/137‑26 (MQ=255)

                                                                                                                                      ttttA‑AATTTGCAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  7:107186‑M1/137‑26 (MQ=255)

                                                                                                                                          a‑aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  1:33371‑M1/138‑31 (MQ=255)

TAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTA‑AATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3598‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`