BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,749	A→T	5.7%	intergenic (+34/‑122)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,749	0	A	T	5.7%	12717.0 / 118.6	4022	intergenic (+34/‑122)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (360/3362); new base T (274/11); total (640/3382)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4519 total aligned reads displayed.

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

 atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                              <  6:152748/138‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  2:105266/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  3:134110/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  4:163540/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  4:47191/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGGACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  8:6468/139‑1 (MQ=255)

  taaatcAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                            <  3:23444/134‑1 (MQ=255)

    aatcAATTAATCAAGACACAGTAAAGAATTTAAGTACCATTACTTATGATCGAGTATTGTCTATTTTTAATAGATATCTCTTATTTTACGGGTGGAAATAATTCTATGAGTCGC‑TTTTTTAATTTTGGATAGTTACACg                                                                                                                                          <  8:159450/136‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGATAGTTACACg                                                                                                                                          <  8:201669/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  1:219243/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                          <  4:121481/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                             <  7:211053/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                             <  8:3409/139‑1 (MQ=255)

                 agCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                             <  3:178832/138‑1 (MQ=255)

                 agCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                             <  2:213354/138‑1 (MQ=255)

                        gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                                      <  6:207859/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  5:85797/139‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  5:110974/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  3:118116/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  5:169130/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCAATACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  7:148457/138‑1 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGTATGGAGAT‑aat                                                                                                                  <  4:205519/139‑2 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑aaa                                                                                                                  <  5:43305/139‑1 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  5:139328/137‑1 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑a                                                                                                                    <  5:213757/137‑1 (MQ=255)

                             cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAatt                                                                                                                <  4:103369/139‑1 (MQ=255)

                             cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAatt                                                                                                                <  3:91232/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  1:121557/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  1:89823/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  3:150166/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  1:206278/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  3:213867/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  2:165469/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  3:49266/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  4:27329/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAattatt                                                                                                             <  3:75918/139‑1 (MQ=255)

                                   caGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAg                                                                                                           <  2:65862/137‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAg                                                                                                           <  3:165519/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAg                                                                                                           <  4:65385/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAg                                                                                                           <  8:219323/138‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGat                                                                                                         <  8:52344/139‑1 (MQ=255)

                                     gTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGata                                                                                                        <  5:100285/139‑1 (MQ=255)

                                     gTACCATTACTTATGAGGAAGGATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGACATAATTCTATGAGTCGC‑GTTTTTAAATTTGGAAAGTTACCCGTTACTAAAGGGAATGGAGAT‑AAATTATTAGata                                                                                                        <  8:173501/139‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGAtata                                                                                                      <  1:188880/138‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTGTTTTAATTTTGAAAATTTAACCTTAAAAAAAGTAAATGAGAT‑AAAATATTTtatata                                                                                                      >  6:45199/1‑138 (MQ=255)

                                               ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGAc                                                                                              <  7:180133/139‑1 (MQ=255)

                                               ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGAc                                                                                              <  5:182240/139‑1 (MQ=255)

                                                   gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTATTTTTGTAAGTTACACGGTTCTTAAGGGTATGGAGTT‑AAATTTTTAGTTTTTCTACTGACACCt                                                                                          >  4:188415/1‑139 (MQ=255)

                                                   gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGAAATTTTCACGGTAGTAAAGGGTATGGTGAT‑TAATTTTTATATATAATAATGACAGCt                                                                                          >  8:140850/1‑139 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCa                                                                                      <  4:146053/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCa                                                                                      <  6:92885/139‑1 (MQ=255)

                                                        aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAAGTTACACGGTTATAAAGGGAATTGTGAT‑AAATTTTTATATATAATACTGACAGCGTccca                                                                                     >  6:191185/1‑137 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACTTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTAGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACATCTTCCaag                                                                                    <  8:79449/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaag                                                                                    <  7:122948/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaag                                                                                    <  6:61239/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaag                                                                                    <  5:25896/139‑1 (MQ=255)

                                                          tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAGTGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                   <  2:179519/139‑1 (MQ=255)

                                                          tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                   <  3:33623/139‑1 (MQ=255)

                                                            ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                 <  7:90856/139‑1 (MQ=255)

                                                               tCTATTTTAAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTa                                                                              <  7:16348/139‑1 (MQ=255)

                                                                 tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTTTTTTGTAATTTTAAAGGTAATAAAAGGGATGGAGATAAAATTTTTTAAAAAAATA‑TGACAGCTTCCaaaaaacgaa                                                                             >  6:10365/1‑133 (MQ=255)

                                                                   tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                            <  7:47962/137‑1 (MQ=255)

                                                                   tttttAATAGTTATCTATTAATTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGTCAGCTTCCAAGAAGCTaaa                                                                            <  6:172614/137‑1 (MQ=255)

                                                                    ttttAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAACGGTTATAAATGGAATGGTGAG‑AAATTATTTTTTATAATAATGTAAGCTTGCCAGAATCGAAaggg                                                                         >  1:46649/1‑137 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                                        <  7:19590/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                                        <  8:106407/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag‑ag                                                                        <  1:57799/138‑1 (MQ=255)

                                                                      ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑Agg                                                                       <  6:124578/138‑1 (MQ=255)

                                                                         aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGATAGTTTCACGTTTATAAAGGGGTTGGTGTT‑AAATTATTTGATTTACTTATGACAGCTTCCAAGAAGCGAAAG‑AGGTgcc                                                                   >  5:56967/1‑139 (MQ=255)

                                                                                    ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGcc                                                            <  1:160475/135‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATCATTCTATGCGTCGC‑TTTTTTAAATTTGGACAGGTACACGTTACTAAAGGCAATGGAGAT‑AAATTATTAGATATACTCCTGACAGCTGCCAAGCAGCTAAAG‑AGGTCCCTAGCGCCTACgg                                                       <  4:83808/138‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACgg                                                       <  1:136125/138‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACgg                                                       <  3:195277/138‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACgg                                                       <  2:146726/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑TTAAGTTAAACCGTACTTAATGGAATTGTGAT‑AAATTATTATATATAATACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACcggg                                                     >  7:136858/1‑139 (MQ=255)

                                                                                          aaCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAATTTAAAGGTGATAAAATTGATTGATGT‑TAATTATTTTATATAATACTGTCAACTACCAATAAACGAAAA‑AGGGCCCAAACCCCGACggg                                                      >  2:33371/1‑135 (MQ=255)

                                                                                           aCGTGAGTAAATATTTCTATTAGTCGC‑TTTTTTAAATTTGGAAATTTACACGTTACTATAGGTAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAAt                                                  <  5:7036/138‑1 (MQ=255)

                                                                                           aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAAt                                                  <  8:121920/138‑1 (MQ=255)

                                                                                                  gaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggta                                          <  5:153951‑M1/139‑6 (MQ=255)

                                                                                                    aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                        <  1:30433‑M1/139‑8 (MQ=255)

                                                                                                        atTCTATGAGTCGC‑TTTTTTATATTTGGAAAGTTACAAGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                                    <  1:173600‑M1/139‑12 (MQ=255)

                                                                                                         ttCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaa                                   <  7:74234‑M1/139‑13 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTAATAAAGGGAATGGATAT‑AAATTATTAGATATACTAATGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGAGCCTACGGGGAATTTGgtgtacattgaaaaagg                              <  3:64370‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  2:48389‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  1:72463‑M1/139‑18 (MQ=255)

                                                                                                               gtgtcGC‑TTTTTTAAATTTGGAAAGTTACACTTTACTAAAGGGAATTGAGAT‑AAATTATTAGATATACTTCTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                              <  1:100491‑M1/136‑18 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:104795‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  7:23761‑M1/138‑18 (MQ=255)

                                                                                                                aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  7:59927‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  2:196400‑M1/139‑20 (MQ=255)

                                                                                                                 gTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag                           <  7:37180‑M1/139‑21 (MQ=255)

                                                                                                                 gTCGC‑TTTTTTAAATTTGGAAATTTACACGTTAATAAAGGGAATTGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  1:14914‑M1/138‑20 (MQ=255)

                                                                                                                 gTCGC‑GTTTTTAAATTTGGAAAGTTACACGTTACTAAAGCGAATGGACAT‑AAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  6:76061‑M1/138‑20 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAA‑GGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                    <  8:188867‑M1/138‑28 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACAAGTTACTAAACGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAACAATCTAAAGCAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  2:129401‑M1/138‑26 (MQ=255)

                                                                                                                         ttttAAATTTGCAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  7:107186‑M1/137‑26 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                 <  5:201167‑M1/138‑31 (MQ=255)

                                                                                                                              aaTTTGTAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt               <  5:124402‑M1/139‑33 (MQ=255)

                                                                                                                                     aaaGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatt        <  6:2283‑M1/139‑40 (MQ=255)

                                                                                                                                           acacGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgataaaggaagagtatgagtattcaacagttccgtg  >  2:86265‑M1/1‑94 (MQ=255)

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAT‑AAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAG‑AGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`