breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
read file | reads | bases | passed filters | average | longest | mapped | |
---|---|---|---|---|---|---|---|
errors | JADR33_R3_A3_F57_I0_R1_S61_L001_R1_001.good.fq | 223,367 | 28,248,488 | 100.0% | 126.5 bases | 139 bases | 94.9% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L001_R2_001.good.fq | 223,367 | 28,250,222 | 100.0% | 126.5 bases | 139 bases | 92.0% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L002_R1_001.good.fq | 226,884 | 28,733,185 | 100.0% | 126.6 bases | 139 bases | 95.7% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L002_R2_001.good.fq | 226,884 | 28,734,373 | 100.0% | 126.6 bases | 139 bases | 93.8% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L003_R1_001.good.fq | 224,560 | 28,381,171 | 100.0% | 126.4 bases | 139 bases | 95.5% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L003_R2_001.good.fq | 224,560 | 28,381,212 | 100.0% | 126.4 bases | 139 bases | 93.6% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L004_R1_001.good.fq | 224,036 | 28,233,313 | 100.0% | 126.0 bases | 139 bases | 96.1% |
errors | JADR33_R3_A3_F57_I0_R1_S61_L004_R2_001.good.fq | 224,036 | 28,235,059 | 100.0% | 126.0 bases | 139 bases | 94.7% |
total | 1,797,694 | 227,197,023 | 100.0% | 126.4 bases | 139 bases | 94.5% |
seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
---|---|---|---|---|---|---|---|
coverage | distribution | BS168_reference_genome | 4,215,606 | 37.7 | 3.5 | 77.5% | Bacillus subtilis subsp. subtilis str. 168 complete genome. |
coverage | distribution | pSIJ663_pHT315_araE_xy | 11,568 | 8059.2 | 339.5 | 22.5% | . |
total | 4,227,174 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
option | limit | actual |
---|---|---|
Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 15836 |
Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 64 |
Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.004 |
reference sequence | pr(no read start) |
---|---|
BS168_reference_genome | 0.89550 |
pSIJ663_pHT315_araE_xy | 0.47209 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
option | value |
---|---|
Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
Junction allow suboptimal matches | FALSE |
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
option | value |
---|---|
Mode | Full Polymorphism |
Ploidy | 1 (haploid) |
Consensus mutation E-value cutoff | 10 |
Consensus frequency cutoff | 0.75 |
Consensus minimum variant coverage each strand | OFF |
Consensus minimum total coverage each strand | OFF |
Consensus minimum variant coverage | OFF |
Consensus minimum total coverage | OFF |
Polymorphism E-value cutoff | 2 |
Polymorphism frequency cutoff | 0.05 |
Polymorphism minimum variant coverage each strand | 2 |
Polymorphism minimum total coverage each strand | OFF |
Polymorphism minimum variant coverage | OFF |
Polymorphism minimum total coverage | OFF |
Polymorphism bias cutoff | OFF |
Predict indel polymorphisms | YES |
Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
program | version |
---|---|
bowtie2 | 2.2.6 |
R | 3.4.4 |
step | start | end | elapsed |
---|---|---|---|
Read and reference sequence file input | 10:15:13 04 Jun 2021 | 10:15:44 04 Jun 2021 | 31 seconds |
Read alignment to reference genome | 10:15:44 04 Jun 2021 | 10:19:50 04 Jun 2021 | 4 minutes 6 seconds |
Preprocessing alignments for candidate junction identification | 10:19:50 04 Jun 2021 | 10:20:16 04 Jun 2021 | 26 seconds |
Preliminary analysis of coverage distribution | 10:20:16 04 Jun 2021 | 10:21:21 04 Jun 2021 | 1 minute 5 seconds |
Identifying junction candidates | 10:21:21 04 Jun 2021 | 10:21:22 04 Jun 2021 | 1 second |
Re-alignment to junction candidates | 10:21:22 04 Jun 2021 | 10:22:41 04 Jun 2021 | 1 minute 19 seconds |
Resolving best read alignments | 10:22:41 04 Jun 2021 | 10:23:18 04 Jun 2021 | 37 seconds |
Creating BAM files | 10:23:18 04 Jun 2021 | 10:24:16 04 Jun 2021 | 58 seconds |
Tabulating error counts | 10:24:16 04 Jun 2021 | 10:24:44 04 Jun 2021 | 28 seconds |
Re-calibrating base error rates | 10:24:44 04 Jun 2021 | 10:24:48 04 Jun 2021 | 4 seconds |
Examining read alignment evidence | 10:24:48 04 Jun 2021 | 10:43:07 04 Jun 2021 | 18 minutes 19 seconds |
Polymorphism statistics | 10:43:07 04 Jun 2021 | 10:43:09 04 Jun 2021 | 2 seconds |
Output | 10:43:09 04 Jun 2021 | 10:43:41 04 Jun 2021 | 32 seconds |
Total | 28 minutes 28 seconds |