Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,480,667	Δ1 bp	intergenic (+42/+83)	zwf → / ← gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,480,667	0	T	.	100.0%	411.9 / NA	91	intergenic (+42/+83)	zwf/gndA	glucose‑6‑phosphate 1‑dehydrogenase (NADP‑dependent)/NADP+‑dependent 6‑P‑gluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (40/51);  total (40/51)

ATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACG  >  BS168_reference_genome/2480538‑2480794                                                                                                                                  |                                                                                                                                aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                       <  5:282379/138‑1 (MQ=255) aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                       <  6:217780/138‑1 (MQ=255) aTCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGc                                                                                                                      >  5:29164/1‑139 (MQ=255) aTCGCCTACCTACGACTCCGGCCCAAGCGGACCCAAAGAATATGATGATCTTTGGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑Ctttttt                                                                                                                          <  2:191967/135‑1 (MQ=255)      cTAACTACGAATCAGCCTCAATGGGACCGAAAGAATCTTATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                 <  3:216742/139‑1 (MQ=255)       tAACTACGAATCAGGCTCATTTTGACCGAAAGAATCTGATGATCTTTTGGTTAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGa                                                                                                                   <  2:372447/136‑1 (MQ=255)       tAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAc                                                                                                                 >  6:360801/1‑138 (MQ=255)        aaCTACGATTCAGGGTAACTGGGACCGAAAGAATATTATTTTTTTTTTGTTAAAGACGGCTTACACTGGTGGAACATATAATATGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  4:5001/139‑1 (MQ=255)        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  4:49067/139‑1 (MQ=255)        aaCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                                >  8:253051/1‑138 (MQ=255)         aCTTCGATTCAGGGTCATTGGGACCGAAATAATGTGATTATCTTTTGGTGAAAGACGGGTTACACTGTTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  8:250800/138‑1 (MQ=255)         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  6:311906/138‑1 (MQ=255)         aCTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACa                                                                                                                <  7:230180/137‑1 (MQ=255)         aCTACGAATCAGGCGCAATGGGACCTAAAGAATCTGATGATCTTTTGGTTAAAGACGGCTTACACTGTGGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  8:261941/138‑1 (MQ=255)          cTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGaaa                                                                                                                 >  1:372447/1‑134 (MQ=255)             ctaaTCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTTGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                                          <  6:406030/137‑1 (MQ=255)             cGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGta                                                                                                          >  6:131285/1‑139 (MQ=255)             cGAATCAGGCTCAATGGGACCGAAAGAATCTGAGGATCTGTTGGTGAAAGACGGCTTACACTGGTGGAACAGATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCAGTTTCGG‑CATTTTTACGATGACCAAAGGta                                                                                                          >  3:222529/1‑139 (MQ=255)              gAATCAGGCTCAATGGGACCG‑AAGAATCAGGAGATGGTTTGGTGAAAGACGGGATTCCCAGGTGGAACATAAAATAAGTAGAAAAAAGCCCAAAATGTTTAAAACCAATTTTCGG‑‑TTTTTTACGGAGAACAAAGGtaa                                                                                                         >  8:270783/1‑137 (MQ=255)                   aGGCTCAATGGGACCTAAAGAATCTGATTATCTTTTGGTGAAAGACGTCTTACACTGGTGGATCATATAATAAGAAGAAAAAAGCCGAAAATGTATAC‑AAGCAATTACGG‑ATTTATTACGCTGAACAAAGGTATaa                                                                                                       >  2:341039/1‑136 (MQ=255)                   aGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGtata                                                                                                        <  1:101580/135‑1 (MQ=255)                     gCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAAtc                                                                                                  >  3:209976/1‑139 (MQ=255)                        cAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGAAGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAAAGATTAC‑ATGCATTTTCGG‑CTTTTTTAAGCTGAACATAGGTATAACAAtc                                                                                                  >  1:105472/1‑136 (MQ=255)                         aaTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                       >  7:20813/1‑114 (MQ=255)                         aaTGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACg                                                                                                                       <  8:20813/114‑1 (MQ=255)                               aCCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTtaataa                                                                                        >  3:268479/1‑139 (MQ=255)                               aCCAAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               >  4:152898/1‑116 (MQ=255)                               aCCAAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACaa                                                                                                               <  3:152898/116‑1 (MQ=255)                                  gAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTg                                                                                                                    >  1:41670/1‑108 (MQ=255)                                  gAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTg                                                                                                                    <  2:41670/108‑1 (MQ=255)                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                     >  4:329284/1‑138 (MQ=255)                                   aaaGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                     >  5:126264/1‑138 (MQ=255)                                      gAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACaa                                                                                     >  1:17922/1‑135 (MQ=255)                                        aTCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                               <  6:92522/139‑1 (MQ=255)                                         tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                               >  8:164154/1‑138 (MQ=255)                                         tCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAccac                                                                               >  6:33058/1‑136 (MQ=255)                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAAcccc                                                                               <  2:17922/134‑1 (MQ=255)                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                             <  6:76647/136‑1 (MQ=255)                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                             <  6:126264/136‑1 (MQ=255)                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                             <  8:315522/136‑1 (MQ=255)                                             atgatCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAg                                                                          >  3:317679/1‑139 (MQ=255)                                                 tCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                       >  3:205179/1‑138 (MQ=255)                                                  cTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCtt                                                                       >  7:123502/1‑137 (MQ=255)                                                    tttGGGGAAAGAGGGCTTACACTGGGTGAACATATAATAAGAAGAAAAAACCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAag                                                                    <  6:37206/138‑1 (MQ=255)                                                        gTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCt                                                                <  7:192820/138‑1 (MQ=255)                                                          gAAAGCCGGCGTACACCGGTGGAACATATAATAACAAGAAAAAAGCCGAAAATGGTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                             <  8:100425/139‑1 (MQ=255)                                                            aaGACGGCTTACACTGGTGGAACATATAAAAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCggg                                                           <  3:11766/139‑1 (MQ=255)                                                                 ggCTTACACTGGTGGAACCTATAATAAGAAGAAAAAAGACGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGATCTTCGGGGGttt                                                      <  6:297539/139‑1 (MQ=255)                                                                  gCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTc                                                     >  8:117014/1‑139 (MQ=255)                                                                           ggtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTa                                            >  5:203343/1‑139 (MQ=255)                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                           >  8:140441/1‑139 (MQ=255)                                                                            gtggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTAc                                           <  3:329284/139‑1 (MQ=255)                                                                             tggAACATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACt                                          >  4:103693/1‑139 (MQ=255)                                                                                aaCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                        >  1:351548/1‑106 (MQ=255)                                                                                aaCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAAAAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCt                                                                        <  2:351548/106‑1 (MQ=255)                                                                                 aCATATAATAAGAATAAACAAGACTTAAATGTCTA‑AAAGCATTTTCGG‑CTTTTTTACTCGTAACAAAGGTATAACCATCTACTTCATAACCACACACCCCAAGCTTAAGAGCTTCGGGTGTTTATATTTATTACTTCAt                                      <  4:222529/139‑1 (MQ=255)                                                                                 aCATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCAt                                      <  4:268479/139‑1 (MQ=255)                                                                                  cATATAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCA‑TTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATc                                     >  6:149286/1‑138 (MQ=255)                                                                                   atatAATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTc                                        >  2:131454/1‑135 (MQ=255)                                                                                    tataATAAGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCa                                   >  7:278695/1‑139 (MQ=255)                                                                                      taatacGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  7:91828/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACATAAGCGTTATTAAAAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTGTTTTGTTTAATTCAACCAt                                  >  8:43180/1‑138 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  3:374556/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  7:134976/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  5:146956/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  7:186065/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  6:9407/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  7:65615/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  3:182459/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 <  5:335683/139‑1 (MQ=255)                                                                                      taataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAAAAAAACCCCCTAAGCTTAAGAGCTTCGGTGGTTTCTATTGATTACTTCATCCAtt                                 <  7:114245/139‑1 (MQ=255)                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 >  4:202307/1‑138 (MQ=255)                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 >  3:203947/1‑138 (MQ=255)                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 >  7:326557/1‑138 (MQ=255)                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCAtt                                 >  7:1280/1‑138 (MQ=255)                                                                                       aataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTc                                <  3:263868/139‑1 (MQ=255)                                                                                        ataaGAAGAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCa                               <  8:373801/139‑1 (MQ=255)                                                                                          aagaagAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGaa                                                                                                                  >  2:260873/1‑54 (MQ=25)                                                                                          aagaagAAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGaa                                                                                                                  <  1:260873/54‑1 (MQ=25)                                                                                           agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                            <  8:278695/139‑1 (MQ=255)                                                                                           agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                            <  7:117014/139‑1 (MQ=255)                                                                                           agaagaAAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTa                            <  7:270783/139‑1 (MQ=255)                                                                                            gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                             >  8:168914/1‑105 (MQ=255)                                                                                            gaagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCg                                                             <  7:168914/105‑1 (MQ=255)                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                             >  5:97853/1‑88 (MQ=255)                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCa                                                                             <  6:97853/88‑1 (MQ=255)                                                                                             aagaaAAAAGCCGAAAATGTTTAC‑AAGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATg                          >  4:378903/1‑139 (MQ=255)                                                                                                    aaGCCGAAAATGTTTTC‑AAGCATATTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATg                   <  5:107653/139‑1 (MQ=255)                                                                                                                  ttacaaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAg      >  3:103880/5‑139 (MQ=255)                                                                                                                    acaaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGt     <  5:360801/136‑1 (MQ=255)                                                                                                                     caaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTAc   <  8:123502/138‑1 (MQ=255)                                                                                                                      aaGCATTTTCGG‑CTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACg  <  3:378903/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                ATCTCCTAACTACGAATCAGGCTCAATGGGACCGAAAGAATCTGATGATCTTTTGGTGAAAGACGGCTTACACTGGTGGAACATATAATAAGAAGAAAAAAGCCGAAATAGTTTACTAAGCATTTTCGGTCTTTTTTACGCTGAACAAAGGTATAACAATCTCCTTAATAACAAAACCCCCAAAGCTTAAGAGCTTCGGGGGTTTCTATTGATTACTTCATCCATTCAGTATGGAAGATGCCTTCTTTGTCAGTACG  >  BS168_reference_genome/2480538‑2480794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑