BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	165,749	Δ1 bp	intergenic (+42/‑5)	rrnI‑5S → / → trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,749	0	C	.	100.0%	422.2 / NA	116	intergenic (+42/‑5)	rrnI‑5S/trnI‑Asn	ribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (56/60); total (56/60)

Only 100 of 116 total aligned reads displayed.

CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGG  >  BS168_reference_genome/165620‑165875

cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                      <  5:30297/139‑1 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                      >  5:81120/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                      >  4:169392/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                      >  3:81989/1‑137 (MQ=255)

  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                                      >  7:58057/1‑137 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTTATTCTACCATGTTTGGTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                                    <  2:256184/136‑1 (MQ=18)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                                    <  1:126742/136‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                                    <  2:90671/136‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                                    <  2:21725/136‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTa                                                                                                                    <  1:206460/136‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                                 <  6:245055/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                                 <  7:368867/139‑1 (MQ=255)

     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                                 <  7:74195/139‑1 (MQ=255)

      cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                                   <  2:264739/136‑1 (MQ=255)

      cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                                <  4:130511/139‑1 (MQ=255)

         aCCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGt                                                                                                             >  3:351363/1‑139 (MQ=255)

            gAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGt                                                                                                          >  5:42105/1‑139 (MQ=255)

            gAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGATTTATAT‑T‑ATTCCGCAGTATCTCAGt                                                                                                             >  1:229689/1‑136 (MQ=21)

             aaCACGGAAGTTAAGCTCTTGAGCGCCGATGGTAGTCGGGGGTTT‑CCGCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                                         <  3:182032/139‑1 (MQ=25)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGATGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  8:139569/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                                 <  8:147541/131‑1 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                                 >  7:147541/1‑131 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  4:47711/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  7:400729/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  6:163718/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  3:153841/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  4:312307/1‑137 (MQ=255)

             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                                           >  7:332053/1‑137 (MQ=255)

               cacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGCCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTggaa                                                                                                         >  8:300494/1‑135 (MQ=25)

                acGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCACAGTgg                                                                                                           >  1:297054/1‑134 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                                     <  2:333413/136‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                                  <  4:81989/139‑1 (MQ=255)

                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                                  <  8:374241/139‑1 (MQ=255)

                     aGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                                     >  2:292151/1‑135 (MQ=255)

                     aGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGc                                                                                                     >  1:227048/1‑135 (MQ=255)

                                  aGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTTTAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGa                                                                                    <  8:176678/139‑1 (MQ=255)

                                   gcgcCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                                      <  2:297054/136‑1 (MQ=255)

                                   gcgcCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                                   <  7:219040/139‑1 (MQ=255)

                                   gcgcCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                                   <  4:359911/139‑1 (MQ=255)

                                   gcgcCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                                   <  8:58057/139‑1 (MQ=255)

                                         aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                             >  6:265433/1‑139 (MQ=255)

                                         aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                             <  3:216917/139‑1 (MQ=255)

                                         aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                             >  6:202350/1‑139 (MQ=255)

                                         aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCAGCAGTAGATCAGTGGTAGAGATATCGGCTGTTAACAGATCGGTCg                                                                             >  7:306411/1‑139 (MQ=37)

                                         aTGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGATTTATAT‑T‑ATTCAGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                                             >  5:358311/1‑139 (MQ=38)

                                         aTGGTAGGCGGGGGTTT‑CCCCCTGGGAGTGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCgg                                                                                <  1:198681/136‑1 (MQ=37)

                                           ggTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGt                                                                            <  5:265433/138‑1 (MQ=255)

                                            gTAGTCGGGGGTTTCCCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAGCGGTc                                                                              >  1:177650/1‑136 (MQ=37)

                                            gTAGTCGGGGGGTT‑CCCCCTGTGAGAGTAGGACGCCGCCACGAAGATTTATTAATGCTACCATGGTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGCGCTATCGGCTGTTAACCGATCGGTc                                                                              <  2:253000/135‑1 (MQ=25)

                                                    gggTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCg                                                                     <  2:349403/136‑1 (MQ=255)

                                                       ttt‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                                  <  6:230225/104‑1 (MQ=255)

                                                       ttt‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                                  >  5:230225/1‑104 (MQ=255)

                                                             cccTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTg                                                          <  5:65483/139‑1 (MQ=255)

                                                                 gtgAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                                        >  7:176583/1‑137 (MQ=255)

                                                                      agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGCGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                >  5:125063/1‑136 (MQ=21)

                                                                      agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                <  3:202735/139‑4 (MQ=255)

                                                                      agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                >  5:184618/1‑136 (MQ=255)

                                                                       gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                               <  3:88825/139‑5 (MQ=21)

                                                                            aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTc                                           >  5:33027/1‑139 (MQ=21)

                                                                            aCGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACATGCGGAGCCATTGCTTc                                           >  7:353738/1‑139 (MQ=21)

                                                                              gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         <  5:202350/139‑1 (MQ=21)

                                                                              gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         <  6:81120/139‑1 (MQ=21)

                                                                              gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         >  4:368836/1‑139 (MQ=21)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGc                                                                                              <  8:349206/84‑1 (MQ=37)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGc                                                                                              >  7:349206/1‑84 (MQ=37)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGa                                                                                    >  8:384599/1‑94 (MQ=25)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGa                                                                                    >  6:268284/1‑94 (MQ=25)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGa                                                                                    <  5:268284/94‑1 (MQ=25)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGtt                                                                       <  7:314325/107‑1 (MQ=255)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGtt                                                                       >  8:314325/1‑107 (MQ=255)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         >  8:247691/1‑137 (MQ=21)

                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATa                                       <  3:157450/139‑1 (MQ=21)

                                                                                  ccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCGCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCAt                                        <  2:299280/136‑1 (MQ=18)

                                                                                   cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         >  2:213136/1‑134 (MQ=18)

                                                                                   cAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                         >  1:36244/1‑134 (MQ=18)

                                                                                       cAGATTTATTAATTCTACCATGTTTGTTTCTGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGCCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                <  5:285697/139‑1 (MQ=18)

                                                                                       cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                <  8:176583/139‑1 (MQ=21)

                                                                                       cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                <  7:92645/139‑1 (MQ=21)

                                                                                               ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                        <  4:96339/139‑1 (MQ=21)

                                                                                               ttaATTCTACCAGGTTTGTATATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTCGGGGCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                        <  6:180787/139‑1 (MQ=18)

                                                                                                    tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                   >  7:162536/1‑139 (MQ=21)

                                                                                                    tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTCGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGTTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                   >  3:98528/1‑139 (MQ=21)

                                                                                                      tACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                  >  4:24847/1‑138 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                   <  2:227048/135‑1 (MQ=18)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTc                  <  3:24847/136‑1 (MQ=18)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                <  8:400729/138‑1 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                <  8:78559/138‑1 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                <  3:169392/138‑1 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                <  7:247691/138‑1 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                <  6:202501/138‑1 (MQ=21)

                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAt               >  7:78559/1‑139 (MQ=21)

                                                                                                           tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTAc                                                             <  2:8283/90‑1 (MQ=17)

                                                                                                           tgtttgttTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTAc                                                             >  1:8283/1‑90 (MQ=17)

                                                                                                             tttgtttATTAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATg              <  2:393850/135‑1 (MQ=18)

                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGt            >  2:776/1‑134 (MQ=18)

                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                >  1:248339/1‑94 (MQ=11)

                                                                                                                   tATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa      >  3:202152/1‑137 (MQ=21)

                                                                                                                     tGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAgg  >  7:308130/1‑139 (MQ=21)

                                                                                                                       aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCt                                                                                             >  6:190098/1‑46 (MQ=11)

                                                                                                                       aaGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCt                                                                                             <  5:190098/46‑1 (MQ=11)

CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTT‑CCCCCTGTGAGAGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGG  >  BS168_reference_genome/165620‑165875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`