| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | pSIJ663_pHT315_araE_xy | 3,843 | Δ5 bp | intergenic (+128/‑24) | eryR → / → ampR | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | pSIJ663_pHT315_araE_xy | 3,842 | 0 | G | . | 100.0% | 152.0 / NA | 34 | intergenic (+127/‑29) | eryR/ampR | –/– |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (22/12); total (22/12) | |||||||||||
| * | pSIJ663_pHT315_araE_xy | 3,843 | 0 | T | . | 100.0% | 152.0 / NA | 34 | intergenic (+128/‑28) | eryR/ampR | –/– |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (22/12); total (22/12) | |||||||||||
| * | pSIJ663_pHT315_araE_xy | 3,844 | 0 | A | . | 100.0% | 152.0 / NA | 34 | intergenic (+129/‑27) | eryR/ampR | –/– |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (22/12); total (22/12) | |||||||||||
| * | pSIJ663_pHT315_araE_xy | 3,845 | 0 | T | . | 100.0% | 152.0 / NA | 34 | intergenic (+130/‑26) | eryR/ampR | –/– |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (22/12); total (22/12) | |||||||||||
| * | pSIJ663_pHT315_araE_xy | 3,846 | 0 | C | . | 100.0% | 152.0 / NA | 34 | intergenic (+131/‑25) | eryR/ampR | –/– |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (22/12); total (22/12) | |||||||||||
AATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA > pSIJ663_pHT315_araE_xy/3711‑3964 ||||| aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTggggt < 1:182126/136‑5 (MQ=255)aataatTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTggggt < 2:99438/136‑5 (MQ=255) aatTGTATGAGTCGCTTTTTTTATTTTGGAAAGTTACAAGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTggggtac < 2:108914/135‑7 (MQ=255) gAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAgg < 7:158732/138‑1 (MQ=255) aGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa < 5:340617/139‑1 (MQ=255) ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAgagtat < 7:213607/137‑1 (MQ=255) aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAgagtatgagta < 8:352997/138‑1 (MQ=255) aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTAtt < 8:295483/139‑1 (MQ=255) cacGTTACTAAAGGGAATGTAGATAAATTATTACATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCg < 1:379333/136‑1 (MQ=255) aaGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCtt > 2:225319/1‑136 (MQ=255) ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTccc > 6:363401/1‑139 (MQ=255) aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCtt > 5:8824/1‑139 (MQ=255) ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAagag < 1:95340/97‑1 (MQ=255) ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAagag > 2:95340/1‑97 (MQ=255) agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAgagt > 2:57520/1‑96 (MQ=255) agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAgagt < 1:57520/96‑1 (MQ=255) agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCtttt > 1:292255/1‑136 (MQ=255) aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCa > 8:232125/1‑139 (MQ=255) actactGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTg > 7:372239/1‑139 (MQ=255) ctactGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCTTGTCGCCCTTATTCCCTTTTTTGAGGCATTTTGCCTTCCTGt > 3:260942/1‑139 (MQ=255) aCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCATCATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGc > 6:290749/1‑139 (MQ=255) aCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACAttt > 5:12906/1‑88 (MQ=255) aCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACAttt < 6:12906/88‑1 (MQ=255) aCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGCGGAATTT‑‑‑‑‑GGGGTCCATTGAAAAAGGAAGAGTATGAGTATTCAACATTTACGTGTCGCCCTTATTCCCGTATTTTCGGAATTTTGCATTCCTGTGTTTGc > 8:165178/1‑139 (MQ=255) gCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGc > 2:63116/1‑136 (MQ=255) ttCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAcc > 3:310947/1‑139 (MQ=255) cTAAAGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCt > 4:247989/1‑139 (MQ=255) aaGAGGTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGt < 7:232125/139‑1 (MQ=255) gTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCAtt > 4:60798/1‑98 (MQ=255) gTCCCTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCAtt < 3:60798/98‑1 (MQ=255) cccTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATGTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGt > 4:36505/1‑138 (MQ=255) ccTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAg > 1:144184/1‑136 (MQ=255) ccTAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAg > 2:370039/1‑136 (MQ=255) tAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACg < 6:150375/124‑1 (MQ=255) tAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACg > 5:150375/1‑124 (MQ=255) tAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGt > 1:370226/1‑135 (MQ=255) tAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTaa > 3:143316/1‑137 (MQ=255) ||||| AATAATTCTATGAGTCGCTTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAA > pSIJ663_pHT315_araE_xy/3711‑3964 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |