BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	557,870	(T)_5→6	intergenic (+421/‑3)	yddT → / → ydzN	putative exported phage protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	557,865	1	.	T	98.2%	586.6 / 1.4	164	intergenic (+416/‑8)	yddT/ydzN	putative exported phage protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (2/1); new base T (81/80); total (83/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

GTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑T‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTT  >  BS168_reference_genome/557728‑557997

 tgAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t                                                                                                                                    >  8:285945/1‑139 (MQ=255)

   aaaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑ttt                                                                                                                                  >  5:353592/1‑139 (MQ=255)

   aaaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑ttt                                                                                                                                  >  8:321677/1‑139 (MQ=255)

   aaaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑ttt                                                                                                                                  >  5:329451/1‑139 (MQ=255)

    aaGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑t                                                                                                                                    >  2:176975/1‑136 (MQ=255)

       tAATGATTTGTATGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑tttttaa                                                                                                                              <  3:405478/139‑4 (MQ=255)

        aaTGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑t‑tttttaa                                                                                                                              <  5:58028/138‑4 (MQ=255)

            aTTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCg                                                                                                                          <  7:94968/139‑1 (MQ=255)

             tttGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCg                                                                                                                          <  3:38239/138‑1 (MQ=255)

             tttGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCg                                                                                                                          <  8:12966/138‑1 (MQ=255)

               tGTAGGATCAGTTAAGAAAGGTTTAATATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTg                                                                                                                       >  5:206185/1‑139 (MQ=255)

               tGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTg                                                                                                                       >  4:59768/1‑139 (MQ=255)

               tGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTg                                                                                                                       >  7:338176/1‑139 (MQ=255)

               tGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTg                                                                                                                       >  6:327394/1‑139 (MQ=255)

                  aGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGa                                                                                                                     <  5:258811/138‑1 (MQ=255)

                      tCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGt                                                                                                                   <  2:117474/136‑1 (MQ=255)

                      tCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTaaa                                                                                                                <  5:305574/139‑1 (MQ=255)

                         gTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTaaa                                                                                                                >  1:322309/1‑136 (MQ=255)

                           tAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATg                                                                                                              >  2:214381/1‑136 (MQ=255)

                             aGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGt                                                                                                            <  2:166210/136‑1 (MQ=255)

                             aGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCa                                                                                                         <  8:351906/139‑1 (MQ=255)

                               aaaGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAAt                                                                                                       >  6:287447/1‑139 (MQ=255)

                                    tttAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  <  4:113098/139‑1 (MQ=255)

                                    tttAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  <  7:321677/139‑1 (MQ=255)

                                    tttAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  >  8:121966/1‑139 (MQ=255)

                                    gttAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTTGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  <  4:385471/138‑1 (MQ=255)

                                       aaGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  >  2:331577/1‑136 (MQ=255)

                                             atatatTGAAAAATTTTATATATTTTCAATAAAATACTCATACTATAATAAATGTATACATATTTTATTGAGATTTAATTAATTAAAAATTA‑G‑TATTATAATTTAGTTTTAGTAAATTGTTCAATTTTTTATGTAtag                                                                                          >  8:319128/3‑138 (MQ=255)

                                             aGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTAtag                                                                                          >  7:103236/1‑138 (MQ=255)

                                                   tGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCAtt                                                                                   >  5:128436/1‑139 (MQ=255)

                                                    gAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATTTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTATCAtt                                                                                   <  4:137810/138‑1 (MQ=255)

                                                    gAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCAtt                                                                                   <  6:329451/138‑1 (MQ=255)

                                                      aaaaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCAtt                                                                                   <  1:158994/136‑1 (MQ=255)

                                                       aaaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAAGTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTg                                                                                  >  1:88068/1‑136 (MQ=255)

                                                        aaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTgg                                                                                 <  1:220535/136‑1 (MQ=255)

                                                        aaTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                              <  7:121966/139‑1 (MQ=255)

                                                         aTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTAGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGa                                                                               <  8:164034/137‑1 (MQ=255)

                                                         aTTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTAGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGa                                                                               >  7:164034/1‑137 (MQ=255)

                                                             tatataTTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTgg                                                                                 <  1:109935/131‑1 (MQ=255)

                                                               tatatTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                       <  7:103846/139‑1 (MQ=255)

                                                               tatatTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                       <  5:286449/139‑1 (MQ=255)

                                                                atatTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAat                                                                          <  1:214381/135‑1 (MQ=255)

                                                                       cAATATAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGAGG‑T‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGTGATCAATATATGGt                                                                   <  2:152837/135‑1 (MQ=255)

                                                                       cAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTtat                                                                <  6:353592/138‑1 (MQ=255)

                                                                                 cTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTAc                                                     <  6:218965/139‑1 (MQ=255)

                                                                                   ggTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACt                                                    >  3:171627/1‑138 (MQ=255)

                                                                                    gTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTat                                                  >  5:348676/1‑138 (MQ=255)

                                                                                    gTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTaa                                                  >  3:370449/1‑139 (MQ=255)

                                                                                       cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                              >  4:23019/1‑108 (MQ=255)

                                                                                       cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                              <  3:23019/108‑1 (MQ=255)

                                                                                       cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTaa                                                  >  1:96970/1‑136 (MQ=255)

                                                                                       cTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATCGTTATAAATTATTTACTaa                                                  >  2:2855/1‑136 (MQ=255)

                                                                                         ataataAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                       <  8:157851/113‑1 (MQ=255)

                                                                                         ataataAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAAcc                                                >  4:339852/1‑136 (MQ=255)

                                                                                          taataaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCaa                                              <  4:184730/137‑1 (MQ=255)

                                                                                            ataaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                              <  7:171605/103‑1 (MQ=255)

                                                                                             taaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGt                                         <  5:145835/139‑1 (MQ=255)

                                                                                             taaATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGt                                         <  3:59768/139‑1 (MQ=255)

                                                                                                    ataCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtac                                   >  8:253282/1‑138 (MQ=255)

                                                                                                    ataCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtac                                   >  7:293424/1‑138 (MQ=255)

                                                                                                    ataCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtac                                   >  4:18059/1‑138 (MQ=255)

                                                                                                     taCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                       <  6:406678/101‑1 (MQ=255)

                                                                                                     taCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAatata                                                                       >  5:406678/1‑101 (MQ=255)

                                                                                                     taCAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGtacta                                 >  6:167407/1‑139 (MQ=255)

                                                                                                       cAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGtttt                                                                                                  >  1:120947/1‑72 (MQ=255)

                                                                                                       cAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTactact                               <  7:154657/139‑1 (MQ=255)

                                                                                                         gATTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCg                             <  8:180426/139‑1 (MQ=255)

                                                                                                          aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGt                            >  5:31711/1‑139 (MQ=255)

                                                                                                          aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGt                            <  3:18059/139‑1 (MQ=255)

                                                                                                          aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGt                            >  3:298419/1‑139 (MQ=255)

                                                                                                          aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGt                            >  4:388496/1‑139 (MQ=255)

                                                                                                          aTTGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTAATACTCGt                            >  8:45602/1‑139 (MQ=255)

                                                                                                           ttGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGtt                           >  5:297926/1‑139 (MQ=255)

                                                                                                            tGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGtt                           >  3:47440/1‑138 (MQ=255)

                                                                                                            tGTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGtt                           >  3:12938/1‑138 (MQ=255)

                                                                                                            tGTACGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGa                                                                               <  8:37263/86‑1 (MQ=255)

                                                                                                            tGTACGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGa                                                                               >  7:37263/1‑86 (MQ=255)

                                                                                                             gTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGta                                                                                             >  2:381891/1‑71 (MQ=255)

                                                                                                             gTATGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGt                            >  1:268710/1‑136 (MQ=255)

                                                                                                               aTGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTg                                                                                  >  1:49468/1‑80 (MQ=255)

                                                                                                               aTGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGtt                           >  2:18886/1‑135 (MQ=255)

                                                                                                               aTGGAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACAAAGAAGTCAGTACTACTCGtt                           >  1:386799/1‑135 (MQ=255)

                                                                                                                 ggAGATGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATTGTTATAAATTATTTACTAACCAAGAATTCAGTACTAATCTTTtaata                      <  6:229037/138‑1 (MQ=255)

                                                                                                                  gagaTGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTaataat                    <  4:370449/139‑1 (MQ=255)

                                                                                                                   agaTGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTtaataa                     >  3:212871/1‑137 (MQ=255)

                                                                                                                   agaTGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTtaata                      <  2:315468/136‑1 (MQ=255)

                                                                                                                    gaTGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGAt                                                                              >  6:284231/1‑79 (MQ=255)

                                                                                                                    gaTGTAATTAATTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAg                  >  6:367050/1‑139 (MQ=255)

                                                                                                                        taattaatTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAg                  <  2:50177/135‑1 (MQ=255)

                                                                                                                        taattaatTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGc                 >  1:351641/1‑136 (MQ=255)

                                                                                                                        taattaatTAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCtt               <  6:297926/138‑1 (MQ=255)

                                                                                                                         aattaattAAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCATTATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATTATAGCt                >  2:341261/1‑136 (MQ=255)

                                                                                                                          attaattaAAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTg            >  5:118539/1‑139 (MQ=255)

                                                                                                                           ttaattaaAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTc              >  2:98103/1‑136 (MQ=255)

                                                                                                                             aattaaAAAGGA‑GTT‑TTTTAATTGAGATGGAGTAAATGGTTCAATGTTTATTGTATAGTAGCATACGGATCAATATATGGTTATAAATTATTAACTAACCAAGAAGTCATTACTACTCTTTTAATAATAGCTTCTGTaa         >  4:395428/1‑139 (MQ=255)

                                                                                                                              attaaAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTaa         <  7:253282/138‑1 (MQ=255)

                                                                                                                               ttaaAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAtt       >  3:254665/1‑139 (MQ=255)

                                                                                                                               ttaaAAAGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAtt       <  3:339852/139‑1 (MQ=255)

                                                                                                                                    aaGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgt     <  2:268710/136‑1 (MQ=255)

                                                                                                                                    aaGGA‑GTT‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAAttgt     <  1:2855/136‑1 (MQ=255)

GTGAAAGTAATGATTTGTAGGATCAGTTAAGAAAGGTTTAAGATTAGATATTGAAAAATTTTATATATTTTCAATAAAATACTGGTACTATAATAAATGTATACAGATTGTATGGAGATGTAATTAATTAAAAAGGA‑G‑T‑TTTTAATTGCGTTGGAGTAAATGGTTCAATGTTTTTTGTATAGTAGCATTGGGATCAATATATGGTTATAAATTATTTACTAACCAAGAAGTCAGTACTACTCGTTTAATAATAGCTTCTGTAATTGTGTT  >  BS168_reference_genome/557728‑557997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`