| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | BS168_reference_genome | 4,155,395 | (A)5→6 | intergenic (‑186/+38) | trnY‑Lys ← / ← purA | tRNA‑Lys/adenylosuccinate synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 4,155,390 | 1 | . | A | 93.8% | 501.3 / 22.9 | 144 | intergenic (‑181/+43) | trnY‑Lys/purA | tRNA‑Lys/adenylosuccinate synthetase |
| Reads supporting (aligned to +/- strand): ref base . (1/8); new base A (60/75); total (61/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
Only 100 of 162 total aligned reads displayed.
GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAAC‑AAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG > BS168_reference_genome/4155253‑4155519 | gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac > 6:165635/1‑138 (MQ=255) ctcATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTc‑aaac > 2:226731/1‑135 (MQ=255) aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aa > 5:212236/1‑137 (MQ=255) aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aa > 6:378282/1‑137 (MQ=255) aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aa > 3:156233/1‑137 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCTATAACTTTTTTGGTTTTTGTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcc > 6:340847/1‑139 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aacaag < 5:74370/137‑6 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aacaa < 3:66930/136‑5 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aacaa < 7:248432/136‑5 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aac < 1:279848/134‑3 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aa < 2:358753/133‑2 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aa < 2:279655/133‑2 (MQ=255) gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcc > 5:109518/1‑139 (MQ=255) tACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGccc < 8:125795/139‑1 (MQ=255) tACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcc < 6:300227/138‑1 (MQ=255) ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcc > 1:361096/1‑136 (MQ=255) ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGc < 1:234727/135‑1 (MQ=255) tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTa > 5:167304/1‑138 (MQ=255) tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTa > 5:191672/1‑138 (MQ=255) tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTa > 3:171139/1‑138 (MQ=255) ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcc < 8:17854/131‑1 (MQ=255) tGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTa > 2:11694/1‑135 (MQ=255) tGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTa > 2:333887/1‑135 (MQ=255) gAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATa < 5:311417/138‑1 (MQ=255) aaGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAgg > 5:136298/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑aaac‑aaaa‑aacaa < 4:203839/120‑5 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 8:238487/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 8:331208/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 6:65209/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 7:276865/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 7:272198/1‑139 (MQ=255) gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 8:172322/1‑139 (MQ=255) ttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTg < 8:330199/138‑1 (MQ=255) ttCCGTAACAACCAGATTTATAATATCACGAACAAAACGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGc < 2:185771/135‑1 (MQ=255) tCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCtt > 1:276625/1‑136 (MQ=255) cGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAg < 6:179952/138‑1 (MQ=255) gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc < 3:67181/139‑1 (MQ=255) gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc < 4:156233/139‑1 (MQ=255) gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc < 5:159272/139‑1 (MQ=255) gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc < 7:354699/139‑1 (MQ=255) gTAACAACGAGATATATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCa < 2:147742/136‑1 (MQ=255) acaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACata > 5:390071/1‑139 (MQ=255) acaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACata > 5:95299/1‑139 (MQ=255) caacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACatat < 4:323407/139‑1 (MQ=255) aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc > 4:342094/1‑134 (MQ=255) aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACatat > 5:370682/1‑138 (MQ=255) aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATAtt < 6:201307/139‑1 (MQ=255) agaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTc < 1:226731/136‑1 (MQ=255) tttATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATTGGGGCTTGCAGACATATTCTAttt < 4:314152/138‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 4:53249/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 6:95299/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 5:192941/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 8:272198/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 7:172322/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 8:22437/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 7:238487/139‑1 (MQ=255) atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACATATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 5:305754/139‑1 (MQ=255) tatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCa < 8:201770/139‑1 (MQ=255) tatCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCa < 3:262026/139‑1 (MQ=255) atCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc < 4:171139/139‑1 (MQ=255) agcACGAACAAATAGAAGCGGCAATAACTTTTTTGGTTTTTTTTCAAATTTTTTTCTCAGACCAACTAATATGCGCCTTTCAAAAA‑AAAA‑ACAACCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCg < 2:146967/134‑1 (MQ=255) tCACGAACAAATAGAAGTGGCATTAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc < 4:6468/138‑1 (MQ=255) tCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc < 6:393140/138‑1 (MQ=255) cACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTAAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 3:281766/135‑1 (MQ=255) cACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTAAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc > 4:281766/1‑135 (MQ=255) aCGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 8:368605/134‑1 (MQ=255) aCGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc < 1:154035/136‑1 (MQ=255) aCGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc < 1:39399/136‑1 (MQ=255) cGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCAc > 3:34045/1‑135 (MQ=255) cGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg > 2:38355/1‑136 (MQ=255) aaaCAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACgg > 1:273358/2‑136 (MQ=255) cAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca < 7:366683/139‑1 (MQ=255) aaGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGaa < 6:109518/139‑1 (MQ=255) aaGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGcccc > 8:27400/1‑84 (MQ=255) tgCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCg < 5:284164/116‑1 (MQ=255) ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCg > 6:284164/1‑117 (MQ=255) ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 7:317489/118‑1 (MQ=255) ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa > 5:207081/1‑138 (MQ=255) ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa > 8:107316/1‑138 (MQ=255) ggCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGAGGCTTTC‑AAACAAAAA‑ACAAGCCCCGTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa > 7:21380/1‑138 (MQ=255) gCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa > 7:196124/1‑139 (MQ=255) cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc < 7:290355/116‑1 (MQ=255) cAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc > 8:290355/1‑116 (MQ=255) aTAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa > 2:364654/1‑136 (MQ=255) ttttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATttgtttgt > 3:32726/1‑138 (MQ=255) ttttGGTTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc > 6:264722/1‑107 (MQ=255) ttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgtttg < 1:333887/135‑1 (MQ=255) ttGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAtttgtttg < 1:38355/135‑1 (MQ=255) tGGTTTTTTTTCATATTTTTTTCTCAGACCACCTTATATGCGGCTATC‑AAAC‑AAAACACAAGCCCCTTCAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg < 3:387052/138‑1 (MQ=255) tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATttgtttgt < 1:11694/135‑1 (MQ=255) tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg < 7:107944/138‑1 (MQ=255) tGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg < 4:390820/138‑1 (MQ=255) ttCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGa < 4:134711/138‑1 (MQ=255) gACAAACTTATATGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGa > 4:38956/1‑138 (MQ=255) atGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGcc > 7:51082/1‑139 (MQ=255) atGCGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGcc > 3:231453/1‑139 (MQ=255) cGGCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt < 4:231453/139‑1 (MQ=255) gCTTTC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAt > 5:358249/1‑85 (MQ=255) ttC‑AAACAAAAA‑ACAAGCCCCTTAAATAGTGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt > 1:333426/1‑134 (MQ=255) tC‑AAACAAAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg > 2:33021/1‑136 (MQ=255) | GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTTC‑AAAC‑AAAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG > BS168_reference_genome/4155253‑4155519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |