BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	166,037	+T	intergenic (+4/‑27)	trnI‑Gly → / → trnI‑Arg	tRNA‑Gly/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	166,037	1	.	T	97.1%	324.2 / 1.7	105	intergenic (+4/‑27)	trnI‑Gly/trnI‑Arg	tRNA‑Gly/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base . (3/0); new base T (34/68); total (37/68)
Fisher's exact test for biased strand distribution p-value = 4.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGAT  >  BS168_reference_genome/165900‑166160

gCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTat                                                                                                                             <  5:186590/139‑2 (MQ=255)

 cTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTatc                                                                                                                            <  4:67431/139‑3 (MQ=255)

 cTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TACA‑TTTatc                                                                                                                            >  8:70614/1‑137 (MQ=255)

    aaaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTatcct                                                                                                                          >  3:271785/1‑134 (MQ=255)

    aaaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTatcc                                                                                                                           <  7:215297/137‑4 (MQ=255)

     aaTGTATTATTACCAAGGTTTCTCATAAGGATAAAGCTTTTTTTATTGCGATATGCGGAATTAGTTCAGTGCTAGAACACCACGTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTaa                                                                                                                        <  8:306411/139‑1 (MQ=21)

     aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTaa                                                                                                                        <  4:111382/139‑1 (MQ=255)

     aaTGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTaa                                                                                                                        <  5:209814/139‑1 (MQ=255)

         tattatTTCGAAGGTTTCTCATAAGGAGAAAGGTTTTTTTATTGCGATATGCTGAAGTAGTTCAGTGGTATATCACGACCTTGCCATGGTGGGGGTCGCTGGTTCGAATCCCGTCTTCCTC‑TCCT‑TTTATCCTAACggg                                                                                                                    <  3:183672/139‑1 (MQ=21)

         tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAAc                                                                                                                       <  1:119003/136‑1 (MQ=255)

         tattatTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACggg                                                                                                                    <  7:22913/139‑1 (MQ=255)

           ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTGCGCGGCCT‑TTTT‑GCTAACGGGa                                                                                                                   >  3:226411/1‑138 (MQ=21)

           ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGat                                                                                                                  <  4:80467/139‑1 (MQ=255)

           ttattaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGa                                                                                                                   >  5:74417/1‑138 (MQ=255)

              ttaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATAtt                                                                                                               <  3:102538/139‑1 (MQ=255)

              ttaCCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGC‑TCCA‑TTTATCCTAACGGGat                                                                                                                  >  1:221126/1‑136 (MQ=255)

                 ccAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGt                                                                                                             >  3:387840/1‑138 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATAtt                                                                                                               <  5:394163/134‑1 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATAtt                                                                                                               >  6:394163/1‑134 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTaaa                                                                                                          >  7:260621/1‑139 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTaaa                                                                                                          >  4:184293/1‑139 (MQ=255)

                   aaGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTGCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGt                                                                                                             <  1:213136/136‑1 (MQ=255)

                    aGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGt                                                                                                             >  1:9703/1‑135 (MQ=255)

                    aGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGAAATGCGGAAGTAGTTCAGTGGAAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGACACCCGTCGTGCGC‑TCCA‑TTTATCCTAACGGGATAtttt                                                                                                             >  2:44225/1‑133 (MQ=21)

                      gTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTaaa                                                                                                          >  1:288866/1‑136 (MQ=255)

                        ttCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGTCGCGGGGTAGACTCCCGTCGTTCGC‑GCCATTTTT‑GCTTGCGGGAGATTGTTaattgga                                                                                                     >  8:159162/1‑137 (MQ=18)

                         tctcATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGGCTTCCGC‑TCCA‑TTTATACTAAAGGGATATTGTAaattgaat                                                                                                    >  8:276/1‑139 (MQ=21)

                             aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAaattgaattg                                                                                                  <  7:158879/137‑1 (MQ=255)

                             aTAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAaattgaatt                                                                                                   <  4:51864/136‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTCGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                            <  2:135781/136‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                            <  2:288866/136‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCg                                                                                            <  1:340198/136‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                         <  6:364060/139‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                         <  8:332053/139‑1 (MQ=255)

                                    gaAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                         <  8:291459/139‑1 (MQ=255)

                                     aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATACTAACGGGATATTGTAAATTGAATTTCGCCCGTAGc                                                                                        >  8:64599/1‑139 (MQ=255)

                                     aaaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATACCGTCTTCCCC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAg                                                                                         >  6:357389/1‑138 (MQ=255)

                                      atgCTTTTTTTATTGCGATATGCGGATGTAGTTCAGTGGTATAACACCACGTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTa                                                                                          <  2:127983/134‑1 (MQ=21)

                                      aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTa                                                                                          <  1:33023/136‑1 (MQ=255)

                                      aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  6:31151/139‑1 (MQ=255)

                                      aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  6:2331/139‑1 (MQ=255)

                                      aaGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTGCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  3:6436/139‑1 (MQ=255)

                                      aaGCTTTTTTTATTGCGATATGCGGAAGGAGTTCAGTGGTAGAACACCACCTTGCCAAGGGGGGGGGCGCGGGGTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  6:389879/139‑1 (MQ=25)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  2:277362/136‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCt                                                                                       <  2:257380/136‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                    <  6:273358/139‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                    <  3:287640/139‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                    <  3:184293/139‑1 (MQ=255)

                                         cTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCaa                                                                                    <  4:156582/139‑1 (MQ=255)

                                            tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAAt                                                                                   <  4:387840/137‑1 (MQ=255)

                                            tttttATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTCGAAACCCCTCGGCCCC‑GCCA‑TTTATCCTAAACGGATATTGTAAATTTAATTGCGACCGTAGCTCaaatt                                                                                 >  4:247015/1‑136 (MQ=18)

                                                 aTTTCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                             <  5:149493/138‑1 (MQ=255)

                                                 aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACTCCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACTGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATa                                                                             <  3:308162/138‑1 (MQ=255)

                                                 aTTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTgg                                                                                <  1:282283/135‑1 (MQ=255)

                                                   tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCC‑TTTATCCTAACGGGAATTTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATaggg                                                                          >  3:256340/1‑137 (MQ=21)

                                                   tGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTACGC‑TCCA‑ATTATCCTAAAGGGATATTGTAAATTGAATTGAGCCCGTAGCTAAATTGGATagag                                                                          >  7:143563/1‑139 (MQ=18)

                                                             ggAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTa                                                                <  8:260621/139‑1 (MQ=255)

                                                              gAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTAc                                                               <  4:215538/139‑1 (MQ=255)

                                                                    gtTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATACTAACGGGATATTGTAAATTTAATTGCGCCCGTAGCTCAATTGGATTGAGCGTTTGACTACGGAta                                                          >  4:385795/1‑137 (MQ=25)

                                                                     ttCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                        <  7:325014/139‑1 (MQ=255)

                                                                           ggTAGAACTCCACCTTGGCATGGTGGGGGTCGCGGGTTCGAATCGCGTCCTCCGC‑TCCA‑TTTATCCTACCGGGATATTGTAAATAGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGtt                                                  <  6:190779/139‑1 (MQ=25)

                                                                             tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGCATCCCGTCTTCCGC‑GCCATTTTT‑CCTAACGGGATATTGTTAATTTAATTTCGCCCGGAGCGCAATTGGATTGAGCGGTTGTCTACGGGTCAAAAGGGTAg                                                >  6:227219/1‑139 (MQ=21)

                                                                             tAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAg                                                <  7:387244/139‑1 (MQ=255)

                                                                               gAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTaa                                                                                                                        >  7:103845/1‑65 (MQ=255)

                                                                               gAACACCACCTTGCCAAGGTGGGGGTCGCGGGGTGGAATACCGGCTTGCGC‑TCCA‑ATTATCCGAACGGGATTTTTTTAAATGAATTTCGCCCGGAGCGCAATTGGATATAGCGTTTGACGACGGATCAAAAGGTTTggg                                              >  4:219565/1‑139 (MQ=16)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                        <  3:391972/124‑1 (MQ=255)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCaa                                                        >  4:391972/1‑124 (MQ=255)

                                                                                    ccaccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCg                                         >  3:340130/1‑139 (MQ=255)

                                                                                     caccTTGCCATGGTGGGGGTCGCGGGTTCGAATCACGTCTTCCGC‑TCCA‑TTTATCCTACCGGGATATTTTAAATTGAATTGCGCCCGTAGCTCAATTGGATTGAGCGTTTGACTACGGATCAAATGGTTAGGGGTTCGa                                        <  6:187376/139‑1 (MQ=25)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGtt                                           <  2:216263/136‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGtt                                           <  1:306204/136‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                        <  4:7612/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                        <  3:124910/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                        <  7:276/139‑1 (MQ=255)

                                                                                     caccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGa                                        <  3:394295/139‑1 (MQ=255)

                                                                                       ccTTGCCACGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGAct                                      <  4:76474/139‑1 (MQ=255)

                                                                                       ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTGCGC‑GCCA‑TTTATCCTAACGGGATATTGTAAATTTAATTTCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTTCGGATCAAAATGTTAGGGGTTGGagg                                      >  3:321586/1‑137 (MQ=25)

                                                                                       ccTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑GCCA‑TTTATCCTAACGGGATATTTTAAATTGAATTGCGCCCGTAGGTGAATTGGTTTGAGCGTTTGGCTACGGATCCAAATGTTAGGGGTTGGAct                                      >  4:331849/1‑139 (MQ=25)

                                                                                           gCCAAGGGGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGActcct                                   <  8:262087/138‑1 (MQ=255)

                                                                                             cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGt                                                                                                             >  1:359395/1‑62 (MQ=255)

                                                                                             cAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGt                                                                                                             <  2:359395/62‑1 (MQ=255)

                                                                                               aGGTGGGGGTCGGGGGTTCGAATCCCTTCTTCCGA‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGATTTGCGCCCGTAGCTCAATTGGATAGAGCGTTTTACTACGGATCAAAAGGTTAGGGGTTCGACTCctct                                 <  2:251863/136‑1 (MQ=21)

                                                                                               aGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCgg                              <  4:241547/139‑1 (MQ=255)

                                                                                                ggTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCtctc                                <  1:97591/136‑1 (MQ=255)

                                                                                                  tGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATGCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCGCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcg                           >  6:180782/1‑139 (MQ=255)

                                                                                                       gTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑ATTATACTAACGGGATATTGTAAATTGAATTGCGCCCGTATCTCAATTGGATATAGCGTTTGACTACGGATGAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgcg                         >  2:2703/1‑136 (MQ=21)

                                                                                                       gTCGCGGGTTCGAATCCCGTCTTCCGC‑ACCA‑TTTATACTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGCGCGTTTGCCTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGgcgcg                         >  2:9044/1‑136 (MQ=21)

                                                                                                       gTCGCGGGTGCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCa                      <  5:117523/139‑1 (MQ=255)

                                                                                                           cgGGTTCGAAACCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGACCGTATCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTGCTCTCGGGCGCGGCctata                  >  7:339165/1‑138 (MQ=21)

                                                                                                              gTTCGAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCttt               <  6:128132/139‑1 (MQ=255)

                                                                                                                  gAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTTAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaa            <  8:143563/138‑1 (MQ=255)

                                                                                                                  gAATCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaat           <  5:404397/139‑1 (MQ=255)

                                                                                                                   aaTCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata          <  4:147984/139‑1 (MQ=255)

                                                                                                                   aaTCCCGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTa             <  2:73270/136‑1 (MQ=255)

                                                                                                                   aaTCCCGGCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaata          <  4:103392/139‑1 (MQ=255)

                                                                                                                        cGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCa                                                                                     <  4:182725/59‑1 (MQ=255)

                                                                                                                        cGTCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCa                                                                                     >  3:182725/1‑59 (MQ=255)

                                                                                                                          tCTTCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGTGCGCGCCATATCTTTTaatataa       >  6:217825/1‑135 (MQ=255)

                                                                                                                           cTTCCGC‑TCCA‑TTTATCCTAACGGGTTATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAatatat  <  6:34846/139‑1 (MQ=255)

                                                                                                                             tCCGC‑TCCA‑TTTATCCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTaatagaa       >  1:194373/1‑132 (MQ=255)

GCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATATGCGGAAGTAGTTCAGTGGTAGAACACCACCTTGCCAAGGTGGGGGTCGCGGGTTCGAATCCCGTCTTCCGC‑TCCA‑TTTA‑CCTAACGGGATATTGTAAATTGAATTGCGCCCGTAGCTCAATTGGATAGAGCGTTTGACTACGGATCAAAAGGTTAGGGGTTCGACTCCTCTCGGGCGCGCCATATCTTTTAATAGAATAGAT  >  BS168_reference_genome/165900‑166160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`