Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 1,073,672 A→T F16I (TTC→ATC)  scoC ← transcriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,073,6720AT100.0% 250.3 / NA 67F16I (TTC→ATC) scoCtranscriptional regulator of extracellular protease production, sporulation and bacilysin production (MarR family)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (31/36);  total (31/36)

GCTCCATTCAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGAAAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTTAATAAA  >  BS168_reference_genome/1073536‑1073806
                                                                                                                                        |                                                                                                                                      
gCTCCATTCAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGCTCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCCGGGTGataa                                                                                                                                      >  6:297550/1‑139 (MQ=255)
   ccATTCAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCa                                                                                                                                   <  6:267745/139‑1 (MQ=255)
      ttCAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCagag                                                                                                                                <  5:402491/139‑1 (MQ=255)
        cAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGc                                                                                                                               <  3:225240/138‑1 (MQ=255)
        cAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGc                                                                                                                               <  8:159021/138‑1 (MQ=255)
             gATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGcttc                                                                                                                            >  1:48363/1‑136 (MQ=255)
              aTACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCttt                                                                                                                         >  4:169799/1‑138 (MQ=255)
               tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca                                                                                                                       <  7:76379/139‑1 (MQ=255)
               tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca                                                                                                                       <  3:400983/139‑1 (MQ=255)
               tACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca                                                                                                                       <  8:230030/139‑1 (MQ=255)
               tACGCAATCCATAAAATATGATGCTCATTAATAATCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca                                                                                                                       <  8:406067/139‑1 (MQ=255)
                aCGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTca                                                                                                                       >  8:115279/1‑138 (MQ=255)
                    aaTCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCAcatca                                                                                                                  <  4:210481/139‑1 (MQ=255)
                    aaTCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCAcatca                                                                                                                  <  6:343611/139‑1 (MQ=255)
                        cATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAgg                                                                                                              >  4:360679/1‑139 (MQ=255)
                         aTAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAggg                                                                                                             <  4:156991/139‑1 (MQ=255)
                                atgatgCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCATTCTCGATCGATTTCCAAAGAGCCTTGCTATGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTc                                                                                                       >  6:374777/1‑138 (MQ=255)
                                 tgatgCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCa                                                                                                     <  7:324378/139‑1 (MQ=255)
                                 tgatgCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCa                                                                                                     <  7:318864/139‑1 (MQ=255)
                                  gatgCTCATTAATATTCAGGTCATACGGTATGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGCGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTcccc                                                                                                    >  5:187902/1‑137 (MQ=255)
                                      cTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGa                                                                                                <  3:169799/139‑1 (MQ=255)
                                         aTTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGAtt                                                                                              >  5:214022/1‑138 (MQ=255)
                                            aaTATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGAtt                                                                                              <  2:344404/135‑1 (MQ=255)
                                                   aGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCAc                                                                                   >  5:203399/1‑139 (MQ=255)
                                                   aGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCAc                                                                                   >  3:252465/1‑139 (MQ=255)
                                                   aGGTCATACGGTTTGAGCCAGTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCAc                                                                                   <  7:379909/139‑1 (MQ=255)
                                                     gTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCt                                                                                 >  8:224200/1‑139 (MQ=255)
                                                      tCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTAc                                                                                        >  2:139457/1‑131 (MQ=255)
                                                      tCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTAc                                                                                        <  1:139457/131‑1 (MQ=255)
                                                        aTACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCTGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCt                                                                                 >  1:328705/1‑136 (MQ=255)
                                                        aTACGGGTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCAcc                                                                                  <  2:152776/135‑1 (MQ=255)
                                                          aCGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTtc                                                                            >  5:254313/1‑139 (MQ=255)
                                                           cGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGc                                                                               <  1:148217/135‑1 (MQ=255)
                                                            ggTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCtt                                                                             >  1:188485/1‑136 (MQ=255)
                                                                    ccATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCtctttttctt                                                                  >  8:133522/1‑139 (MQ=255)
                                                                       ttGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTTGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCtctttttctt                                                                  <  1:186988/136‑1 (MQ=255)
                                                                       ttGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               <  7:224200/139‑1 (MQ=255)
                                                                       ttGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCCGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               <  5:297550/139‑1 (MQ=255)
                                                                       ttGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTCGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               <  3:260587/139‑1 (MQ=255)
                                                                        tgctgcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACTTCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               <  3:393103/138‑1 (MQ=255)
                                                                         gctgcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCtttttctttttc                                                              <  7:102287/138‑1 (MQ=255)
                                                                         gctgcCAATCCTTCTCGATCGATTTCCAAAGAGCATTGCTACGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTGCACATCATAGGGCGGTTCCACTCGATACATTACCTCACCTGCAGCtctttttctttt                                                                >  2:45007/1‑136 (MQ=255)
                                                                            gcCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCta                                                          >  6:205827/1‑139 (MQ=255)
                                                                             ccAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCtat                                                         <  6:203399/139‑1 (MQ=255)
                                                                             ccAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCtat                                                         <  4:252465/139‑1 (MQ=255)
                                                                             ccAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCtat                                                         >  7:208576/1‑139 (MQ=255)
                                                                                aTCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCtattat                                                      <  7:133522/139‑1 (MQ=255)
                                                                                         gatcgatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCaat                                                <  3:360679/136‑1 (MQ=255)
                                                                                         gatcgatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCa                                                  <  2:48363/134‑1 (MQ=255)
                                                                                          atcgatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATACATTACGTCACCTGCTTCTCTTTTTATTTTTCTGTATTATGCCaat                                                >  1:166924/1‑135 (MQ=255)
                                                                                           tcgatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               >  6:314518/1‑119 (MQ=255)
                                                                                           tcgatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTctttttcttttt                                                               <  5:314518/119‑1 (MQ=255)
                                                                                             gatTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCaataaaata                                          <  8:208576/138‑1 (MQ=255)
                                                                                                             tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGTCGGTTCCACTCGATTCATTACGTCACCTGCTTCTATTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATg                          >  5:262313/1‑138 (MQ=255)
                                                                                                             tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTc                                                                                             >  7:59613/1‑71 (MQ=255)
                                                                                                             tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTc                                                                                             <  8:59613/71‑1 (MQ=255)
                                                                                                             tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTGTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATg                          >  7:44046/1‑138 (MQ=255)
                                                                                                             tGCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATtaata                                >  2:91581/1‑132 (MQ=255)
                                                                                                              gCTAAGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGa                         <  7:115279/138‑1 (MQ=255)
                                                                                                               cTAAGCTGAGCCATTTTATGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTAAGTCACATGCTTCTCTTTATATTTTTCTGTATTATGACAATAAAATAAATATTAATAAAGATGaa                        >  4:319480/1‑138 (MQ=255)
                                                                                                                  aGCTGAGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTAGGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTTTGCCAAAAAAATAAAGATTAATAAAGATGAAAATg                    >  6:38034/1‑139 (MQ=255)
                                                                                                                       aGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAg                >  3:396969/1‑138 (MQ=255)
                                                                                                                       aGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAg                >  5:348978/1‑138 (MQ=255)
                                                                                                                       aGCCATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTATCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAg                >  5:196036/1‑138 (MQ=255)
                                                                                                                          cATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGAtt                                    >  2:5880/1‑115 (MQ=255)
                                                                                                                          cATTTTCTGGGTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGAtt                                    <  1:5880/115‑1 (MQ=255)
                                                                                                                                    gTGATAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTtaa      >  2:120835/1‑135 (MQ=255)
                                                                                                                                    gTGATAACCAGAGCTTCTTTCACATCATAGGGCGGATCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTTAATaaa  <  5:205827/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GCTCCATTCAATTGATACGCAATCCATAAAATATGATGCTCATTAATATTCAGGTCATACGGTTTGAGCCATTGCTGCCAATCCTTCTCGATCGATTTCCAAAGAGCCTTGCTAAGCTGAGCCATTTTCTGGGTGAAAACCAGAGCTTCTTTCACATCATAGGGCGGTTCCACTCGATTCATTACGTCACCTGCTTCTCTTTTTCTTTTTCTCTATTATGCCAATAAAATAAAGATTAATAAAGATGAAAATGTAAGAATTTTTTAATAAA  >  BS168_reference_genome/1073536‑1073806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑