Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	1,474,890	C→T	L45L (TTG→TTA)	kre ←	regulator of transcription factor ComK function via modulation of mRNA stability

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	1,474,890	0	C	T	100.0%	278.9 / NA	78	L45L (TTG→TTA)	kre	regulator of transcription factor ComK function via modulation of mRNA stability
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/48);  total (30/48)

TCTTTAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTC  >  BS168_reference_genome/1474756‑1475021                                                                                                                                       |                                                                                                                                    tcttTAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCCCGTCAGATTGCTGTTGGATGATACTTTGATGTTTTGAAAAATGATGCCCAATTTTCTTTCCTTTACGTTCTTTTACAAGCTTTTGTAAAg                                                                                                                                 >  8:95452/1‑139 (MQ=255)        ttGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCGGttt                                                                                                                          >  3:16784/1‑139 (MQ=255)             ggCATGTGAAAATAGTTGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTca                                                                                                                     <  4:29079/139‑1 (MQ=255)             ggCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTca                                                                                                                     <  7:361398/139‑1 (MQ=255)              gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGtttttt                                                                                                                       <  2:285615/136‑1 (MQ=255)              gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  5:337319/139‑1 (MQ=255)              gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  7:240529/139‑1 (MQ=255)              gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  3:129870/139‑1 (MQ=255)               cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  8:171120/139‑1 (MQ=255)               cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGACTGCTGGGGGCGGACCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCATTACCTTCTTTGACAAGCTGTTGTAAATCCCGTTTTTTcagc                                                                                                                   <  4:94307/139‑1 (MQ=255)                   tgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  1:374736/135‑1 (MQ=255)                    gAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  1:8340/1‑133 (MQ=255)                     aaaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGa                                                                                                                <  2:242780/136‑1 (MQ=255)                      aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                             <  7:354513/138‑1 (MQ=255)                       aaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGata                                                                                                              <  2:204207/136‑1 (MQ=255)                            tagtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAtt                                                                                                      >  3:213935/1‑139 (MQ=255)                             agtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  7:91627/124‑1 (MQ=255)                             agtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  8:91627/1‑124 (MQ=255)                             agtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTa                                                                                                        >  2:90762/1‑136 (MQ=255)                              gtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                     <  6:279811/138‑1 (MQ=255)                              gtCTCCGATTGAGATAAGCACGTCCGAGTGCTGTTGGCTGAACCTTTGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                     <  7:255312/138‑1 (MQ=255)                                 tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   >  8:266115/1‑121 (MQ=255)                                 tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  7:266115/121‑1 (MQ=255)                                 tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACATTGGATTTTTTGAAAAATGAAGCCCTAGTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAggg                                                                                                 >  5:70826/1‑139 (MQ=255)                                   cGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTCAAAAATGAAGCACTATTTTCTTTCCTTTACCTTCGTTGACAAGCTTTAGTAAAGCCCTTTTTTTCAGAAGATAAAGGTATGTAGGGtt                                                                                               >  6:2961/1‑139 (MQ=255)                                           aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  6:166172/1‑110 (MQ=255)                                           aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  5:166172/110‑1 (MQ=255)                                           aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCgg                                                                                       >  6:376699/1‑139 (MQ=255)                                                   gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt                                                                                  <  2:8340/136‑1 (MQ=255)                                                   gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt                                                                                  <  2:383041/136‑1 (MQ=255)                                                   gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGGTGGGGGCGGTTTTTGCa                                                                               <  8:350764/139‑1 (MQ=255)                                                    tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCGGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCAt                                                                              >  6:134191/1‑139 (MQ=255)                                                    tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTATTGTTGGGGGCGGTTTTTGCAt                                                                              >  5:201171/1‑139 (MQ=255)                                                    tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCAt                                                                              >  4:112686/1‑139 (MQ=255)                                                       gATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    <  4:293641/98‑1 (MQ=255)                                                       gATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                    >  3:293641/1‑98 (MQ=255)                                                       gATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTATTTTCAGCAGATATAGGGAGTTAGGGTAGGGGGCGGTGTTTGc                                                                                >  1:357553/1‑134 (MQ=255)                                                        aTTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTGGTTGCTTTACCTTCTTTGACAAGCGTTTGTAAAGCCCGTTTTTTGCGGAGGTATAGGTTTTTTGGGTTGGGGGGGGGTTTTGGATg                                                                             >  2:129578/1‑136 (MQ=255)                                                        aTTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCg                                                                          >  5:34896/1‑139 (MQ=255)                                                            cTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc                                                                      <  4:400319/139‑1 (MQ=255)                                                            cTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc                                                                      <  8:20078/139‑1 (MQ=255)                                                              gTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    <  6:344607/139‑1 (MQ=255)                                                               ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCGGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    <  4:16784/138‑1 (MQ=255)                                                               ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   <  6:100049/91‑1 (MQ=255)                                                               ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                   >  5:100049/1‑91 (MQ=255)                                                               ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    <  6:34896/138‑1 (MQ=255)                                                                 ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAg                                                                  <  3:157480/138‑1 (MQ=255)                                                                 ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAGATAGTTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCCCAgt                                                                 >  6:7557/1‑139 (MQ=255)                                                                      aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                               <  8:161243/120‑1 (MQ=255)                                                                      aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                               >  7:161243/1‑120 (MQ=255)                                                                      aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                            >  6:273944/1‑139 (MQ=255)                                                                         cTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTc                                                                                                                      >  2:55633/1‑78 (MQ=255)                                                                         cTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTc                                                                                                                      <  1:55633/78‑1 (MQ=255)                                                                           tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCt                                                       >  7:298826/1‑139 (MQ=255)                                                                                    tGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGa                                                  <  1:4503/135‑1 (MQ=255)                                                                                    tGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAg                                               <  7:206390/138‑1 (MQ=255)                                                                                                cccTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGtt                                  <  7:123500/139‑1 (MQ=255)                                                                                                cccTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGtt                                  <  5:21574/139‑1 (MQ=255)                                                                                                     ttttCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTg                             <  3:331548/139‑1 (MQ=255)                                                                                                          tttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    >  7:178368/1‑95 (MQ=255)                                                                                                          tttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    <  8:178368/95‑1 (MQ=255)                                                                                                            tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATc                       <  6:337988/138‑1 (MQ=255)                                                                                                            tcCTTTACCTTCTTTGACAAGCTTATGTAAAGCACGTTTTTTCAGGAGATATATGTATTTAGGGGTGGGGGCGGTTTTTGCATGGCGGATCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATc                       <  4:283395/138‑1 (MQ=255)                                                                                                             ccTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATTGCGGTTAACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTAGGCTGCAGa                         >  1:100069/1‑135 (MQ=255)                                                                                                             ccTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     >  7:29391/1‑139 (MQ=255)                                                                                                              cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATc                       >  2:327128/1‑136 (MQ=255)                                                                                                              cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                     <  5:376699/138‑1 (MQ=255)                                                                                                               tttACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt                                                                                  <  2:92612/76‑1 (MQ=255)                                                                                                               tttACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt                                                                                  >  1:92612/1‑76 (MQ=255)                                                                                                                  aCCTTCTTTGACTAGCTTTTGTAAAGCCAGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGTGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  6:91726/138‑1 (MQ=255)                                                                                                                  aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTATAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  3:112686/138‑1 (MQ=255)                                                                                                                  aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGGGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                 <  7:245156/138‑1 (MQ=255)                                                                                                                  aCCTTCTTTGACAAGCTTTTGTAAAGACCGTTTTATCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTTATCTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTGCGt                 <  8:213382/138‑1 (MQ=255)                                                                                                                          tGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCTGTTTTTGCATGGCGGTTCACAGTGTAAACTTCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCa          <  6:70826/137‑1 (MQ=255)                                                                                                                             ctaGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTGTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtc     <  5:2961/137‑1 (MQ=255)                                                                                                                                gCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    >  3:157635/1‑73 (MQ=255)                                                                                                                                gCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                    <  4:157635/73‑1 (MQ=255)                                                                                                                                    ttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc  <  1:129578/135‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TCTTTAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTC  >  BS168_reference_genome/1474756‑1475021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑