Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	127,648	A→G	E696G (GAA→GGA)	rpoC →	RNA polymerase (beta' subunit)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	127,648	0	A	G	100.0%	287.0 / NA	75	E696G (GAA→GGA)	rpoC	RNA polymerase (beta' subunit)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/42);  total (33/42)

AACTAAAGCTGGTATTACAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGAACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGCATCT  >  BS168_reference_genome/127514‑127778                                                                                                                                       |                                                                                                                                   aaCTAAAGCTGTTATTACAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACg                                                                                                                                 <  5:343135/138‑1 (MQ=255)             tATTACAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTc                                                                                                                    <  3:213902/139‑1 (MQ=255)                 aCAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  8:31806/138‑1 (MQ=255)                 aCAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  8:310231/138‑1 (MQ=255)                 aCAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  4:286450/138‑1 (MQ=255)                 aCAGTTGGGGTTTATGACATCGTAGTACTAGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  4:323162/138‑1 (MQ=255)                  cAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGATAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCa                                                                                                                   <  1:261841/135‑1 (MQ=255)                  cAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtct                                                                                                                <  8:382313/138‑1 (MQ=255)                  cAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtct                                                                                                                <  4:241627/138‑1 (MQ=255)                  cAGTTGGGGTTTCTGACATCGTCGAACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtct                                                                                                                <  8:271091/138‑1 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTTTGAGAGAGTCATCTCTATCt                                                                                                          >  5:272514/1‑139 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 >  3:224791/1‑132 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  4:224791/132‑1 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 >  4:198007/1‑132 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  6:244457/132‑1 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 >  5:244457/1‑132 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  3:198007/132‑1 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  3:323162/1‑139 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  6:322494/1‑139 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  7:155818/1‑139 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  3:275561/1‑139 (MQ=255)                       ggggTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  3:234448/1‑139 (MQ=255)                          gTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  1:369637/1‑136 (MQ=255)                          gTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCt                                                                                                          >  1:178016/1‑136 (MQ=255)                          gTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTg                                                                                                         >  8:118238/1‑137 (MQ=255)                             tCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAt                                                                                                                  >  8:239538/1‑125 (MQ=255)                             tCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAt                                                                                                                  <  7:239538/125‑1 (MQ=255)                              cTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGtg                                                                                                    <  7:118238/138‑1 (MQ=255)                              cTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGtg                                                                                                    <  3:174795/138‑1 (MQ=255)                                     cgtcgtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATgaga                                                                                                                         >  3:158784/1‑110 (MQ=255)                                     cgtcgtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATgaga                                                                                                                         <  4:158784/110‑1 (MQ=255)                                     cgtcgtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCaa                                                                                              >  7:8776/1‑137 (MQ=255)                                     cgtcgtACTCGAGTATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAATGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCaaa                                                                                             <  6:64554/138‑1 (MQ=255)                                         gtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        <  8:155818/139‑1 (MQ=255)                                         gtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        <  4:371951/139‑1 (MQ=255)                                         gtACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        <  5:322494/139‑1 (MQ=255)                                         gtACTCGAGGATAAACAAGTAATTCTTGAGGAAGCGCATAGCAAAGGTGACAACGTTATGAAGCATTTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        <  8:312342/139‑1 (MQ=255)                                                 tgatAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGCCGTTATcc                                                                                  >  7:105096/1‑137 (MQ=255)                                                 tgatAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCGTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        >  4:235384/1‑131 (MQ=255)                                                 tgatAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCATATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACg                                                                                        <  3:235384/131‑1 (MQ=255)                                                    tAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATAAGGAGTGCTGCAAAAGACGTTATCCaa                                                                                >  2:191040/1‑136 (MQ=255)                                                       aCAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGc                                                                             <  1:147475/136‑1 (MQ=255)                                                         aaGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACt                                                                        >  4:332817/1‑139 (MQ=255)                                                           gAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGTAAACtga                                                                      >  4:405196/1‑139 (MQ=255)                                                              aTTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATACAAGGCAAACtga                                                                      >  1:113307/1‑136 (MQ=255)                                                              aTTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTGATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGTAGTGCTGCAAAAGACGTTATCCAAGGCAAACtgatt                                                                    >  5:22030/1‑137 (MQ=255)                                                               ttCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGaa                                                                  <  3:283819/139‑1 (MQ=255)                                                               ttCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGaa                                                                  <  4:234448/139‑1 (MQ=255)                                                                        aaGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTtga                                                         <  8:265810/139‑1 (MQ=255)                                                                         aGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTtgat                                                        >  7:268095/1‑139 (MQ=255)                                                                          gcgcAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTtgat                                                        >  8:114417/1‑138 (MQ=255)                                                                              aaagcaaagTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACt                                                   <  8:228285/139‑1 (MQ=255)                                                                                  caaagTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTc                                                  <  1:357003/136‑1 (MQ=255)                                                                                     agTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCg                                            <  3:332817/139‑1 (MQ=255)                                                                                          aCAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCg                                            <  8:274574/134‑1 (MQ=255)                                                                                          aCAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCg                                            >  7:274574/1‑134 (MQ=255)                                                                                            aaCGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCt                                        <  2:369637/136‑1 (MQ=255)                                                                                            aaCGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACa                                     <  7:114417/139‑1 (MQ=255)                                                                                              cGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTAc                                      <  1:42883/136‑1 (MQ=255)                                                                                              cGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACatg                                   <  8:153070/139‑1 (MQ=255)                                                                                                 tATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 <  4:257240/58‑1 (MQ=255)                                                                                                 tATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCAtc                                                                                                                 >  3:257240/1‑58 (MQ=255)                                                                                                 tATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACatgat                                 >  3:81091/1‑138 (MQ=255)                                                                                                  aTGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACatgatg                                <  4:154400/138‑1 (MQ=255)                                                                                                   tGAAGCACTTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAg                              <  8:312436/139‑1 (MQ=255)                                                                                                   tGAAGCAATTCCGCCGCGTTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACTTTATACAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAg                              <  7:232484/139‑1 (MQ=255)                                                                                                    gAAGCAATTCCGCCGGGGTCTTATATCTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGAAATAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAg                              <  6:272514/138‑1 (MQ=255)                                                                                                        cAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTTTCTGGAGTGCTCCAAAAGACCTTATACAAGGCAAACTGATGAAATCACTAGATGAACGCAACCCGACCTACATTATTATTGaaa                         >  4:79555/1‑137 (MQ=255)                                                                                                        cAATTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATAACTTGAGGAAATCAACCCGATCTACATGATGAGTGAct                         >  7:126484/1‑139 (MQ=255)                                                                                                          aTTCCGCCGCGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCGATCTGGAGTGCTGCAAAAGACGTTATCCAACGCAAACAGATGAAATCACGTGATGAACTCAACCCGATATACATGATGAGTGActct                       >  3:226612/1‑139 (MQ=255)                                                                                                               gccgcGGGCTTCTCACAGAAGTATGACGCTATGAGAGAGTCATCTCTATGTGGAGTGCTGAAAAAGAAGTTATCAAAGGCAAACTGATGAAATCATTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGc                  <  6:22030/139‑1 (MQ=255)                                                                                                                ccgcGGTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGcc                 >  7:373952/1‑139 (MQ=255)                                                                                                                     gTCTTATCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATGTGGAGTGGGGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGCTGAACTCAACCCGATCGACATGATGAGTGACTCTGGTGCCCgggg            >  4:19946/1‑139 (MQ=255)                                                                                                                           tCACTGAAGAAGGACGCTATGAGAGAGTCATCTCTATATGGAGTGCTGCAAAAGACGTTTTCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATTATGAGTGACTCTTGTTCCCGTGGTATCg       >  8:175175/1‑138 (MQ=255)                                                                                                                              cTGAAGAAGGACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGTAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGCATc   <  3:405196/139‑1 (MQ=255)                                                                                                                                gaagaaggaCGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTtgat                                                        >  7:403710/1‑84 (MQ=255)                                                                                                                                gaagaaggaCGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTtgat                                                        <  8:403710/84‑1 (MQ=255)                                                                                                                                gaagaaggaCGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGCATCt  >  4:359087/1‑138 (MQ=255)                                                                                                                                gaagaaggaCGCTATGAGAGAGTCATCGCTATCTGGAGTGCTGCAAAAGGCGGTATCCAAGGCAAACTGATGAAATCACTTGCTGCACTCCCCCCGCTCGACATGATGAGTGCCTCTGGTGCCCGTGGTACCGCATCt  >  4:63852/1‑138 (MQ=255)                                                                                                                                   gaaggaCGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGc      >  2:205069/1‑131 (MQ=255)                                                                                                                                   gaaggaCGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGc      <  1:205069/131‑1 (MQ=255)                                                                                                                                   gaaggaCGCTATGAGAGAGTCATCTCAATCGGGAGTGCTGCAAAAGACGTTATCCCACGCAAACTGATGAAAACACTTGATGAACTCCACCCGATCTACATTATGAGTGACTCTGGTGCCCGTGGTAACGCATCt  >  2:11049/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                   AACTAAAGCTGGTATTACAGTTGGGGTTTCTGACATCGTCGTACTCGATGATAAACAAGAAATTCTTGAGGAAGCGCAAAGCAAAGTTGACAACGTTATGAAGCAATTCCGCCGCGGTCTTATCACTGAAGAAGAACGCTATGAGAGAGTCATCTCTATCTGGAGTGCTGCAAAAGACGTTATCCAAGGCAAACTGATGAAATCACTTGATGAACTCAACCCGATCTACATGATGAGTGACTCTGGTGCCCGTGGTAACGCATCT  >  BS168_reference_genome/127514‑127778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑