BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	143,659	C→T	S100F (TCT→TTT)	rpsE →	ribosomal protein S5

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	143,659	0	C	T	98.4%	438.2 / ‑2.2	124	S100F (TCT→TTT)	rpsE	ribosomal protein S5
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (56/65); total (58/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

Only 100 of 124 total aligned reads displayed.

AAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAG  >  BS168_reference_genome/143524‑143794

aagTACCAGCAGCGAGTCGCAAAGCTGTTGAAGAGGCGAAAAAGACTTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACCCGAAATCATCGGACGTTTCGGGGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  8:83627/139‑1 (MQ=255)

    aacataaGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                   >  4:117038/6‑138 (MQ=255)

    aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAgg                                                                                                                                   >  4:78782/1‑138 (MQ=255)

    aCCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGt                                                                                                                                  >  3:120995/1‑139 (MQ=255)

         aaGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTg                                                                                                                              >  3:197092/1‑138 (MQ=255)

          aGCGATTAGCAAAGCTGTTTAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCAACACTAAATCAGCGGACGTTTCTGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTg                                                                                                                              >  3:387008/1‑137 (MQ=255)

             gaatCGCAAAGCAGTTGAAGATGCGAAAAAGAATTTCATTGAAGTACCAATGTTTGGAACTACAATTCCACACGAATTCATCGGACGTTTCGGTGCAGGTAAAATCTTGTTAAAACATCCGTTTGAAGGTACTGGAGt                                                                                                                          >  6:337004/4‑138 (MQ=255)

             gATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGt                                                                                                                          >  3:298695/1‑138 (MQ=255)

              aTTCGCAAAGCTGTTTAAGATGCGATAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTa                                                                                                                        <  3:117038/139‑1 (MQ=255)

                tCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTAGTGGAGt                                                                                                                          >  2:243952/1‑135 (MQ=255)

                 cGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTa                                                                                                                        >  1:99083/1‑136 (MQ=255)

                     aaGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTgg                                                                                                                 <  6:216548/139‑1 (MQ=255)

                      aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCt                                                                                                                   <  1:76270/136‑1 (MQ=255)

                      aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                <  4:298695/139‑1 (MQ=255)

                      aGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                <  7:222601/139‑1 (MQ=255)

                           ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                >  5:178524/1‑134 (MQ=255)

                           ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGa                                                                                                                <  6:178524/134‑1 (MQ=255)

                           ttGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGcc                                                                                                            <  3:14032/138‑1 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTg                                                                                                                  >  4:103417/1‑125 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTg                                                                                                                  <  3:103417/125‑1 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACg                                                                                                     <  7:388875/138‑1 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                    >  8:234713/1‑139 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                    >  5:360740/1‑139 (MQ=255)

                                  tGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGt                                                                                                    >  7:26113/1‑139 (MQ=255)

                                   gCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   >  4:299989/1‑139 (MQ=255)

                                    ctaaaaaGAATTTGATTGAAGTACCAATTGTTGGAACTACAATTCCACACGAACTCATAGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGATTTTGAAGTTACTGGAGTTATCTCTGGAGGCCATGTACTTg                                                                                                   <  4:387008/136‑1 (MQ=255)

                                    cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   <  8:66172/138‑1 (MQ=255)

                                    cGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGc                                                                                                  <  5:35024/139‑1 (MQ=255)

                                    agAAAAAGAATTTGCTTGAAGTACCAATGGTTGTAACAACAATTCCACACGATTTCATCGGAAGTTTCGGTGCAGGTAACATCTTGTTAAAACCTTCTTTTGAAGGTACTGGAGTTATCGCTCGAGGCCCTGTACGTg                                                                                                   <  6:59627/137‑1 (MQ=255)

                                     gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGATATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCg                                                                                                 >  4:172587/1‑139 (MQ=255)

                                     gAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCg                                                                                                 <  4:113457/139‑1 (MQ=255)

                                      aaaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   >  2:51344/1‑136 (MQ=255)

                                      aaaaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTg                                                                                                   >  2:275709/1‑136 (MQ=255)

                                        aaaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGct                                                                                                 >  2:107814/1‑135 (MQ=255)

                                         aaGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTAc                                                                                             <  4:188193/139‑1 (MQ=255)

                                                   ttgaAGTACCACTGGGTGGAACTACAATTCCACACGACATCATCGGACGTGTCGGTGCAGGTAACATATTGTTAAAACCTGCTTTTGAAGTTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctagc                                                                                   <  8:397514/139‑1 (MQ=255)

                                                   ttgaAGTACCAATGGATGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGGGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagct                                                                                      <  1:178561/136‑1 (MQ=255)

                                                        gTACCAATGGTTGGAACTACAATTCCACACGGAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTg                                                                                 <  2:403553/136‑1 (MQ=255)

                                                        gTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtg                                                                              <  6:69179/139‑1 (MQ=255)

                                                         tACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgagt                                                                             <  3:78782/139‑1 (MQ=255)

                                                            cAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTGTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGc                                                                          >  4:249427/1‑139 (MQ=255)

                                                             aaTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTa                                                                            <  2:17014/136‑1 (MQ=255)

                                                              aTGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTg                                                                        <  6:406884/139‑1 (MQ=255)

                                                                 gTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGa                                                                                                                                      <  7:4592/74‑1 (MQ=255)

                                                                   tGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                   >  5:13691/1‑139 (MQ=255)

                                                                    ggAACTACCATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAAAATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCTGTACTTGAGCTAGCTGGTGTAGATGATATc                                                                   <  8:60094/138‑1 (MQ=255)

                                                                     gAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGatat                                                                    >  1:198621/1‑136 (MQ=255)

                                                                      aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACtt                                                                                           <  1:52455/112‑1 (MQ=255)

                                                                      aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACtt                                                                                           >  2:52455/1‑112 (MQ=255)

                                                                      aaCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATc                                                                   >  1:40876/1‑136 (MQ=255)

                                                                      aaCTACAATACCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGagctag                                                                                    >  8:403702/1‑119 (MQ=255)

                                                                       aCTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTGGCTGGTGTAGCTGATATcc                                                                  <  2:23778/136‑1 (MQ=255)

                                                                         tACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTATAACCTGCTTTTGAAGGTACTTGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             <  5:337004/139‑1 (MQ=255)

                                                                         tACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTAc                                                                                                      <  1:286334/98‑1 (MQ=255)

                                                                              ttCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCt                                                             >  1:208350/1‑134 (MQ=255)

                                                                                ccACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtgt                                                                             <  7:340133/116‑1 (MQ=255)

                                                                                ccACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtgt                                                                             >  8:340133/1‑116 (MQ=255)

                                                                                ccACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      >  6:145744/1‑139 (MQ=255)

                                                                                ccACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      >  5:374105/1‑139 (MQ=255)

                                                                                 cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                         <  1:80439/135‑1 (MQ=255)

                                                                                 cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGt                                                         <  1:48568/135‑1 (MQ=255)

                                                                                 cacaCGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      <  3:62058/138‑1 (MQ=255)

                                                                                  acacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCt                                                       <  2:353429/136‑1 (MQ=255)

                                                                                  acacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTa                                                    <  8:241110/139‑1 (MQ=255)

                                                                                   cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGgtgt                                                                             >  7:298219/1‑113 (MQ=255)

                                                                                   cacGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAg                                                   >  5:15366/1‑139 (MQ=255)

                                                                                    acGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                  <  4:367052/139‑1 (MQ=255)

                                                                                      gAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACt                                                                                                                               >  6:285478/1‑60 (MQ=255)

                                                                                                   gTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGAt                                   <  4:115705/139‑1 (MQ=255)

                                                                                                    tttCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTg                                                                                                                  >  8:333931/1‑59 (MQ=255)

                                                                                                    tttCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTg                                                                                                                  <  7:333931/59‑1 (MQ=255)

                                                                                                    tttCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGAt                                   >  7:28968/1‑138 (MQ=255)

                                                                                                      tCGGTGCAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCaa                                >  4:29498/1‑139 (MQ=255)

                                                                                                       cGGTGCAGGTAACATCTTGTTACAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAAc                               <  6:39488/139‑1 (MQ=255)

                                                                                                            cAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAt                          <  8:158323/139‑1 (MQ=255)

                                                                                                            cAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAt                          <  5:145744/139‑1 (MQ=255)

                                                                                                            cAGGTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGAt                          <  7:234713/139‑1 (MQ=255)

                                                                                                               gTAACATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCtt                                                      >  3:328769/1‑108 (MQ=255)

                                                                                                                   cATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCa                   <  6:15366/139‑1 (MQ=255)

                                                                                                                   cATCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCa                   <  7:9916/139‑1 (MQ=255)

                                                                                                                    aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCACCATGATTCGTGcca                  >  8:287744/1‑137 (MQ=255)

                                                                                                                    aTCTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCaa                  >  6:238353/1‑139 (MQ=255)

                                                                                                                      cTTGTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAac                 >  4:118782/1‑138 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTaa                                                           >  5:228957/1‑93 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTa                                                    <  4:14757/100‑1 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTa                                                    >  3:14757/1‑100 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                  <  8:259697/102‑1 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAgg                                                  >  7:259697/1‑102 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                        <  3:65686/112‑1 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAacaca                                        >  4:65686/1‑112 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAaca                >  2:256407/1‑136 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACtt             >  8:164853/1‑139 (MQ=255)

                                                                                                                         gTTAAAACCTGCTTTTGAAGGTACTGGAGTTAACGCTGGAGGCCCTGTAGGTGCGGTACTTGAGCTAGATGGTGTAGATGATTTCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCTTGCAccagtg             >  3:32491/1‑136 (MQ=255)

                                                                                                                          ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAaca                <  1:256407/135‑1 (MQ=255)

                                                                                                                          ttAAAACCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACtt             <  3:118782/138‑1 (MQ=255)

                                                                                                                            ataaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGAGATCCTTTCTAAGTCGTTAGGGTCTAACACACCGATCAACATGATTCGTGCAACACTTCa           <  4:280248/136‑1 (MQ=255)

                                                                                                                              aaCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAgg        >  4:264334/1‑139 (MQ=255)

                                                                                                                               aCCTGCTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGt       <  3:168731/139‑1 (MQ=255)

                                                                                                                                    cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAg  >  6:311889/1‑139 (MQ=255)

                                                                                                                                    cTTTTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAg  >  8:64794/1‑139 (MQ=255)

AAGTACCAGAAGCGATTCGCAAAGCTGTTGAAGATGCGAAAAAGAATTTGATTGAAGTACCAATGGTTGGAACTACAATTCCACACGAAATCATCGGACGTTTCGGTGCAGGTAACATCTTGTTAAAACCTGCTTCTGAAGGTACTGGAGTTATCGCTGGAGGCCCTGTACGTGCGGTACTTGAGCTAGCTGGTGTAGCTGATATCCTTTCTAAGTCTTTAGGTTCTAACACACCGATCAACATGATTCGTGCAACACTTCAAGGTTTAAG  >  BS168_reference_genome/143524‑143794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`