| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 166,344 | Δ1 bp | 100% | intergenic (+16/‑156) | trnI‑Ala → / → rrnH‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 166,344 | 0 | G | . | 100.0% | 96.4 / NA | 57 | intergenic (+16/‑156) | trnI‑Ala/rrnH‑16S | tRNA‑Ala/ribosomal RNA‑16S |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (31/26); total (31/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTA > BS168_reference_genome/166209‑166462 | aTGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgtta > 3:308237/1‑136 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCCGCGGTTCGTTCCCGCTAGGCTCCCCCCAAAGTTTTTAAAagagttat > 5:264012/1‑135 (MQ=11) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 1:137557/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 7:43558/1‑135 (MQ=17) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGATTTTAAAAAAgttat > 3:111701/1‑135 (MQ=12) tGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGAACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAgttat > 7:246656/1‑135 (MQ=12) gggTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTAAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTAtt < 3:75173/138‑1 (MQ=12) gTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGAc > 3:3266/1‑139 (MQ=17) cAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACttt > 4:333218/1‑138 (MQ=17) aGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTga < 2:92565/139‑1 (MQ=17) gTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTg > 4:185400/1‑136 (MQ=17) cGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCCCCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag > 2:344386/1‑139 (MQ=12) gAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag < 8:233107/138‑1 (MQ=17) gAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGaagaag < 5:362690/138‑1 (MQ=17) aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg < 1:14268/139‑1 (MQ=17) aaTCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTg < 6:209510/139‑1 (MQ=17) cTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa < 3:110779/138‑1 (MQ=18) cTGTCTTACCGACCATTTTTTATTGGGCCTTAGCTCAGCTGGGAGAGCGACTGCTTTGCACGCAGGAGTTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTATTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACa < 1:80422/138‑1 (MQ=11) tCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACAtt < 4:401010/137‑1 (MQ=21) tCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTg > 2:275851/1‑138 (MQ=21) cTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta < 3:368572/139‑1 (MQ=21) cccGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGtat < 8:94725/137‑1 (MQ=21) cccGACCATTTTTTATGGGGCCTAAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGta < 6:240490/136‑1 (MQ=255) cATTTTTTATGGGGCGTTAGCTCAGCTGGGAGAGAGCCTGCTTTGCTCGCAGGAGGTCAGCCGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGATGTGACATTGTATACtaata > 7:71432/1‑138 (MQ=11) ttttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑TTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAg > 6:248851/1‑139 (MQ=2) ttttATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGt < 4:203343/138‑1 (MQ=11) gggCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTCTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTaa > 4:85083/1‑139 (MQ=2) ccTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACaa < 7:54010/139‑1 (MQ=11) aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaa < 3:390877/139‑1 (MQ=11) aGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGATTTAACAAAGCGGcca > 6:211423/1‑138 (MQ=2) gCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTATAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAACGCGGacaag > 4:326359/1‑138 (MQ=2) cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaa > 7:300040/1‑138 (MQ=11) cTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGacaaa > 7:190434/1‑138 (MQ=11) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt > 4:210628/1‑139 (MQ=11) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt > 4:69438/1‑139 (MQ=11) gcgcCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGAt > 6:59140/1‑139 (MQ=11) gcCTGCTTTGAACGCAGGATGTCAGCGGTTCGAACCCGCTAGCCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCt > 8:69553/1‑139 (MQ=11) cTGCTTTGCACGCAGGAGGGCAGCGGGTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCttt < 8:295984/139‑1 (MQ=2) tGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGaaaa < 3:333218/138‑1 (MQ=11) gCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCGCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAACGCGGACAAACAAAATGATCTTTGAAAAc > 2:382871/1‑138 (MQ=2) cAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACa < 6:399941/139‑1 (MQ=11) aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc > 6:246926/1‑137 (MQ=11) aggaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAAc > 8:423093/1‑137 (MQ=11) gaggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa < 7:8254/139‑1 (MQ=11) aggTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa > 6:260257/1‑138 (MQ=11) gTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa > 5:68906/1‑138 (MQ=11) gCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACg < 1:275851/139‑1 (MQ=11) ttCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa > 2:210369/1‑128 (MQ=11) ttCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGa < 1:210369/128‑1 (MQ=11) ttCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTAcc > 1:142620/1‑139 (MQ=11) ttCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTAcc < 5:122780/139‑1 (MQ=11) ttCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTCTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTAcc < 3:85083/139‑1 (MQ=2) cGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg > 8:230650/1‑139 (MQ=11) cGATCCCGCCAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTg > 8:382651/1‑139 (MQ=2) gATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGt < 4:3266/139‑1 (MQ=11) cGCTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAAtt < 3:185400/139‑1 (MQ=11) cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa < 2:386835/120‑1 (MQ=11) cTAGGCTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACa > 1:386835/1‑120 (MQ=11) cTCCACCAAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGtttt > 6:77690/1‑139 (MQ=11) caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa > 7:371957/1‑138 (MQ=11) caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa > 1:223634/1‑138 (MQ=11) caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa > 2:48555/1‑138 (MQ=11) caccaAAAGTTTTTAAAAAA‑GTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTa > 6:294488/1‑138 (MQ=11) | ATGGGGTCGCAGGTTCGAATCCTGTCTTCCCGACCATTTTTTATGGGGCCTTAGCTCAGCTGGGAGAGCGCCTGCTTTGCACGCAGGAGGTCAGCGGTTCGATCCCGCTAGGCTCCACCAAAAGTTTTTAAAAAAGGTTATTGACTTTGAAGAAGTGACATTGTATACTAATAAAGTTGCTTTAACAAAGCGGACAAACAAAATGATCTTTGAAAACTAAACAAGACAAAACGTACCTGTTAATTCAGTTTTTA > BS168_reference_genome/166209‑166462 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |