| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | BS168_reference_genome | 1,487,388 | Δ1 bp | 8.9% | coding (351/477 nt) | fldN → | short‑chain flavodoxin (acts in lipid desaturation) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | BS168_reference_genome | 1,487,388 | 0 | C | . | 8.9% | 149.9 / 6.9 | 45 | coding (351/477 nt) | fldN | short‑chain flavodoxin (acts in lipid desaturation) |
| Reads supporting (aligned to +/- strand): ref base C (22/19); new base . (2/2); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAACCG > BS168_reference_genome/1487269‑1487525 | aTTCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAAtt > 3:255407/1‑138 (MQ=255) ttCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGTGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTg > 3:301111/1‑138 (MQ=255) ttCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTg > 4:177510/1‑138 (MQ=255) ttCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTATTATCCAAAGTTTTGCGAAGCGGTGAATTTTTTAAATTTCATTATTCAAGAGGAGGGAGCTGCTGTTTACAAGGAAACACTAAAAAttt > 4:17003/1‑137 (MQ=255) tAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGaaa < 4:255407/138‑1 (MQ=255) tAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGaaa > 5:419956/1‑138 (MQ=255) aCAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATg < 8:423684/139‑1 (MQ=255) aGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATg > 8:78672/1‑137 (MQ=255) gCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGACACACTAAAATTTGAATTAGCGCCTGAAACAGATg > 6:86691/1‑136 (MQ=255) gCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATg > 4:192282/1‑136 (MQ=255) gCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGAtgt > 7:283593/1‑139 (MQ=255) cTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGAtgtg < 1:244072/139‑1 (MQ=255) cGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGa < 1:164534/139‑1 (MQ=255) ttATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGAtgtg < 3:179151/118‑1 (MQ=255) ttATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGAtgtg > 4:179151/1‑118 (MQ=255) aTCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGc > 6:89087/1‑136 (MQ=255) tacAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGACTTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCgag < 4:348452/136‑1 (MQ=255) tacAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCAAGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCgag < 3:333561/136‑1 (MQ=255) tCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGACAGCTGCCGAGCGTTTGCgag < 6:404462/138‑1 (MQ=255) aaGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAAAAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGtt > 4:190045/1‑139 (MQ=255) ttGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCTAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCtt > 8:94002/1‑139 (MQ=255) ttGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCtt > 7:376172/1‑139 (MQ=255) cGAAGCGGTGAATTTGTTCAATGTCATGATGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCa < 3:24464/139‑1 (MQ=255) gCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATggg < 1:212646/139‑1 (MQ=255) gCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATggg < 7:270248/139‑1 (MQ=255) cGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCa < 1:204430/134‑1 (MQ=255) cGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCa > 2:204430/1‑134 (MQ=255) cGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATggg < 4:301111/138‑1 (MQ=255) gTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAg < 7:130304/139‑1 (MQ=255) gTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAg < 5:103732/139‑1 (MQ=255) gTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTatat > 1:369823/1‑138 (MQ=255) aaTGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACa < 3:177510/139‑1 (MQ=255) tgctgcAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAATATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGaaa > 7:395117/1‑138 (MQ=255) cAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATc > 7:120649/1‑138 (MQ=255) agagGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAg < 3:206061/113‑1 (MQ=255) agagGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAg > 4:206061/1‑113 (MQ=255) agagGCGGGAGCTGCTGTTTA‑CAGGAAACACTAAAAATTGAATTAGCGCCTGaaa > 4:23836/1‑55 (MQ=255) agagGCGGGAGCTGCTGTTTA‑CAGGAAACACTAAAAATTGAATTAGCGCCTGaaa < 3:29526/55‑1 (MQ=255) agagGCGGGAGCTGCTGTTTA‑CAGGAAACACTAAAAATTGAATTAGCGCCTGaaa > 4:29526/1‑55 (MQ=255) agagGCGGGAGCTGCTGTTTA‑CAGGAAACACTAAAAATTGAATTAGCGCCTGaaa < 3:23836/55‑1 (MQ=255) gAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCAt > 7:391794/1‑138 (MQ=255) gctgTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAggg > 7:230017/1‑139 (MQ=255) tACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAAcc > 2:292479/1‑139 (MQ=255) tACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAAc < 7:338841/138‑1 (MQ=255) aCCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGTGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAAcc < 8:395117/138‑1 (MQ=255) ccAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAACCg < 7:94002/138‑1 (MQ=255) | ATTCAGCTTAATGGTTTAAAAACAGCCTGCTTCGGGTCTGGCGATTATTCTTATCCAAAGTTTTGCGAAGCGGTGAATTTGTTCAATGTCATGCTGCAAGAGGCGGGAGCTGCTGTTTACCAGGAAACACTAAAAATTGAATTAGCGCCTGAAACAGATGAAGATGTGGAAAGCTGCCGAGCGTTTGCGAGAGGTTTTCTTGCATGGGCAGATTATATGAACAAGGAAAAAATCCATGTTTCATAAAGGGGCAACCG > BS168_reference_genome/1487269‑1487525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |