Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 165,749 Δ1 bp 100% intergenic (+42/‑5) rrnI‑5S → / → trnI‑Asn ribosomal RNA‑5S/tRNA‑Asn

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome165,7490C.100.0% 286.7 / NA 86intergenic (+42/‑5)rrnI‑5S/trnI‑Asnribosomal RNA‑5S/tRNA‑Asn
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (42/44);  total (42/44)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCA  >  BS168_reference_genome/165620‑165861
                                                                                                                                  |                                                                                                                 
cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        <  4:134017/139‑1 (MQ=255)
cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        <  2:267197/139‑1 (MQ=255)
cGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        <  7:305635/139‑1 (MQ=255)
  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        >  4:61355/1‑137 (MQ=255)
  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        >  1:308346/1‑137 (MQ=255)
  ttCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGGTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAg                                                                                                        >  8:254811/1‑137 (MQ=255)
   tCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAg                                                                                                     >  6:371970/1‑139 (MQ=255)
     ccATACCGAACCCGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                   <  6:215982/139‑1 (MQ=255)
     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTTGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                   <  1:273278/139‑1 (MQ=255)
     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                   <  8:244011/139‑1 (MQ=255)
     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                   <  4:340919/139‑1 (MQ=255)
     ccATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCt                                                                                                   <  7:399507/139‑1 (MQ=255)
      cATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTc                                                                                                  <  3:61355/139‑1 (MQ=255)
         aCCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGt                                                                                               >  6:15460/1‑139 (MQ=255)
         aCCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGt                                                                                               >  7:346405/1‑139 (MQ=255)
            gAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                             >  8:389824/1‑138 (MQ=255)
             aaaacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                           <  7:164811/136‑1 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                             >  4:309337/1‑137 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                             >  3:180673/1‑137 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTgg                                                                                             >  5:371413/1‑137 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                           <  2:308346/139‑1 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                           <  1:379252/139‑1 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                           <  3:309337/139‑1 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTa                                                                                           >  6:134740/1‑139 (MQ=255)
             aaCACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTACGCAGTAGCTCAGTgg                                                                                             >  5:328400/1‑137 (MQ=255)
              acacGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTag                                                                                          <  5:134740/139‑1 (MQ=255)
                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                    >  7:79626/1‑139 (MQ=255)
                    aaGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCACCCTGTGAGAGTA‑GGTCGCCGCCTAGAAGATTTATTAATTCTACCTTGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTTGTAGAGCTAt                                                                                    <  4:397533/139‑1 (MQ=25)
                       ttAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCGCAGTGGTAGAGCTATCgg                                                                                 <  6:40914/139‑1 (MQ=255)
                            ctctTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGt                                                                             >  1:243956/1‑138 (MQ=255)
                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGATCTATCGGCGGTTAAcc                                                                        >  4:5399/1‑139 (MQ=37)
                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                        >  8:307641/1‑139 (MQ=255)
                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                        >  8:396624/1‑139 (MQ=255)
                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                        >  4:202626/1‑139 (MQ=255)
                                tCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAAcc                                                                        >  6:31271/1‑139 (MQ=255)
                                  aGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGa                                                                      <  5:15460/139‑1 (MQ=255)
                                   gcgcCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                     <  7:161417/139‑1 (MQ=255)
                                    cgccgcTGGGACGCGGGGGGTTCCCCCAGTGTGCGTACGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGAt                                                                     <  1:353639/139‑1 (MQ=25)
                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                               >  8:250766/1‑139 (MQ=255)
                                         aTGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCg                                                               >  4:71101/1‑139 (MQ=255)
                                          tGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGt                                                              <  8:113672/139‑1 (MQ=255)
                                          tGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGt                                                              <  7:389824/139‑1 (MQ=255)
                                           ggTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGt                                                              <  2:173647/138‑1 (MQ=255)
                                               gTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGtt                                                         <  3:360286/139‑1 (MQ=255)
                                                 cGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTc                                                        >  8:399636/1‑138 (MQ=255)
                                                   ggggTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGGTTATGCAGCGTTATAT‑T‑ATTCCGCAGTCGCTCAGTGGTAGAGCTATCGGCTGGTACCCCATCGGTCGTAGGTTCGaa                                                     >  5:53842/1‑139 (MQ=25)
                                                       tttCCCCCTGTGAGAGTA‑GGAAGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCTCAGTAGCTAAGTGGTAGAGATATCGGCTGATAACCAATCGGTCGTAGGTTCGAATcc                                                  >  2:144042/1‑138 (MQ=25)
                                                          cccccTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTAcc                                              >  4:201083/1‑139 (MQ=255)
                                                            cccTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTg                                            <  7:250766/139‑1 (MQ=255)
                                                                gtgAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCg                                          >  7:119578/1‑137 (MQ=255)
                                                                     agTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                  >  8:288312/1‑136 (MQ=255)
                                                                      gTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                 <  8:346405/139‑5 (MQ=21)
                                                                          ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                <  1:144042/138‑1 (MQ=21)
                                                                          ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCt                               >  2:35849/1‑139 (MQ=21)
                                                                          ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCt                               >  1:120453/1‑139 (MQ=21)
                                                                          ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                 >  4:170124/1‑132 (MQ=21)
                                                                          ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                 <  3:170124/136‑5 (MQ=21)
                                                                              gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                           <  8:249319/139‑1 (MQ=21)
                                                                              gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                           <  8:243282/139‑1 (MQ=21)
                                                                                cgccTATCAGATTTATTAATTCTACCATGTTTGTTTATGCAGCTTTATAT‑T‑ATTCCGCAGTAGCTCATTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTTCTTCCATa                         <  8:252435/139‑1 (MQ=18)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                    <  2:422505/80‑1 (MQ=38)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTAt                                                                                    >  1:422505/1‑80 (MQ=38)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                >  2:145612/1‑132 (MQ=18)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                <  1:145612/132‑1 (MQ=18)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                           >  7:295132/1‑137 (MQ=21)
                                                                                cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                           >  2:326898/1‑137 (MQ=21)
                                                                                 gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAg                        <  6:288755/139‑1 (MQ=21)
                                                                                     aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTca                    <  8:119578/139‑1 (MQ=21)
                                                                                     aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGAGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTCCGTAGCCATTGCTTCCATAGCt                      >  2:266707/1‑137 (MQ=21)
                                                                                       cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                  <  7:396624/139‑1 (MQ=21)
                                                                                          aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg               >  6:75782/1‑139 (MQ=21)
                                                                                          aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg               >  1:32428/1‑139 (MQ=21)
                                                                                               ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          >  8:91494/1‑139 (MQ=21)
                                                                                               ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGGTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          <  7:254811/139‑1 (MQ=21)
                                                                                                    tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt     >  3:347296/1‑139 (MQ=21)
                                                                                                     cTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt     <  4:12116/138‑1 (MQ=21)
                                                                                                       aCCATGTTTGGATATGACGCTTTATAT‑TC‑TTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa  <  4:347296/139‑1 (MQ=18)
                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCTGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa  <  1:326898/138‑1 (MQ=21)
                                                                                                        ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa  <  5:371970/138‑1 (MQ=21)
                                                                                                        ccATGTTTGTTTATGAAGCTTTAAAT‑T‑ATTCCGCAGTTGCTCAGTGGTAGAGCTATCGGATGTTAACCGATCGGGCGGAGGTTCGAATCCTACCTTCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGATCACTTCCa  <  6:85860/138‑1 (MQ=18)
                                                                                                             tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa  <  4:277206/133‑1 (MQ=18)
                                                                                                             tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa  >  3:277206/1‑133 (MQ=18)
                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          >  2:116244/1‑122 (MQ=17)
                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          >  2:110084/1‑122 (MQ=17)
                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          <  1:116244/122‑1 (MQ=17)
                                                                                                                gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGGTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag          <  1:110084/122‑1 (MQ=17)
                                                                                                                                  |                                                                                                                 
CGTTCCCATACCGAACACGGAAGTTAAGCTCTTCAGCGCCGATGGTAGTCGGGGGTTTCCCCCTGTGAGAGTA‑GGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCA  >  BS168_reference_genome/165620‑165861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑