BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	4,155,390:1	+A	100%	intergenic (‑181/+43)	trnY‑Lys ← / ← purA	tRNA‑Lys/adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	4,155,390	1	.	A	97.9%	330.8 / ‑1.8	94	intergenic (‑181/+43)	trnY‑Lys/purA	tRNA‑Lys/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (41/51); total (41/53)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 108 total aligned reads displayed.

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAAC‑A‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG  >  BS168_reference_genome/4155253‑4155519

gTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑caaac                                                                                                                                      >  6:125074/1‑138 (MQ=255)

      aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aa                                                                                                                             >  8:30678/1‑137 (MQ=255)

      aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aa                                                                                                                             >  8:338710/1‑137 (MQ=255)

      aTCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aa                                                                                                                             >  3:100148/1‑137 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATTCGGCTTT‑Ctaac‑a‑aaa‑aaaaagcc                                                                                                                       >  1:406062/1‑135 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          <  2:100263/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          <  7:110923/136‑5 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGAACAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcc                                                                                                                        >  7:31480/1‑139 (MQ=255)

           gTACTCTATTGAAGCAGTTTCCGGAACAACGAGATTTATTATATCTCGAAAAATTTGAAGTGGTTATAACTTATTTGT‑TTTTTTTTCTTATTTTTTTCTCAGACCAACTTATATGGGGCTTT‑CAaac‑ataaa‑acaa                                                                                                                           <  8:149970/136‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcccc                                                                                                                      <  4:313165/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcccc                                                                                                                      <  2:296584/138‑1 (MQ=255)

              ctctATTGAAGCAGTTTACGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcccc                                                                                                                      <  6:316837/138‑1 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCTTATTTTTTTCTCAGACCAACTTATATGCGGCTTG‑CAAACAA‑AAA‑ACAAGCCCCTTa                                                                                                                   >  6:416670/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          >  3:217470/1‑126 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          <  4:217470/130‑5 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTa                                                                                                                   >  3:267366/1‑138 (MQ=255)

                 tATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTa                                                                                                                   >  1:70450/1‑138 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          >  7:218776/1‑124 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          <  8:218776/128‑5 (MQ=255)

                   ttGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTaaa                                                                                                                 >  1:388789/1‑138 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCt                                                                                                         >  7:363489/1‑139 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCACCTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCtt                                                                                                        >  7:182108/1‑139 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          <  2:37307/120‑5 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑Caaac‑a‑aaa‑aacaa                                                                                                                          >  1:37307/1‑116 (MQ=255)

                           gTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCtt                                                                                                        >  2:341111/1‑139 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  2:388789/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  3:23461/139‑1 (MQ=255)

                            tttCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTAATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  4:121649/139‑1 (MQ=255)

                            gttCCTTAACAAATAGATTTATAATATCACTAACAAATAGAAGTGTCAATAACTTTTTTTT‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CACACAA‑AAA‑ACAAGCCCCTTAAATAGGGGATTg                                                                                                       <  1:66286/138‑1 (MQ=255)

                              tCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGGGGCAATAACTTTTTTGGTTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  7:423889/138‑1 (MQ=255)

                                cGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAg                                                                                                    <  1:157707/138‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  <  4:267366/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  <  5:125074/139‑1 (MQ=255)

                                 gTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  <  2:289640/139‑1 (MQ=255)

                                    acaacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACata                                                                                               >  3:216580/1‑139 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATa                                                                                                               <  2:323138/121‑1 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATa                                                                                                               >  1:323138/1‑121 (MQ=255)

                                      aacGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                             <  5:229458/139‑1 (MQ=255)

                                      aaagagaTTTATAATATCACGAACAAATTGATGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                             <  4:92497/136‑1 (MQ=255)

                                       acGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGGTTTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATAtt                                                                                             <  8:363489/139‑1 (MQ=255)

                                        cGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       <  7:345490/127‑1 (MQ=255)

                                        cGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTg                                                                                                       >  8:345490/1‑127 (MQ=255)

                                          agaTTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACAGATTCTAt                                                                                         >  4:7820/1‑139 (MQ=255)

                                             tttATAATATCCGTAAAAATTCTATGTGGCTATTACTTTTTTGG‑TTTTTTTTAATATTTTTTTCTCAGACAAAGTTATTTGGGGTTTTAAAAAA‑A‑AAA‑ACAAGCCCCTTATATCGGGTATTTCAGACATATTCTAttt                                                                                       <  2:406062/138‑1 (MQ=255)

                                                ataataTCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGt                                                                                    <  5:297064/138‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  7:95777/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  8:342593/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  6:115832/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  5:163972/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  4:100148/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  2:35945/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  3:240980/139‑1 (MQ=255)

                                                   atatCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTATCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATCGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                <  5:270119/139‑1 (MQ=255)

                                                    tatCACGAACAAATAGAAGTGGAAATAACTTTTTTGG‑‑TTTTTTTCATATTATTTTCTCAGACCAACTTATATGCGGGTTT‑CAAACAA‑AAA‑ACAAGCCCGTTAAATTGGGGCTTGCAGACATATTCTATTTAGTTCGCAc                                                                              <  4:298314/139‑1 (MQ=255)

                                                      tatcGAACAATTAGATGTGGGAATTACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACAAACTTATATGCGGCTTT‑CAAAC‑A‑AAATCCAAGCCGCTTAATTAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             <  6:350244/136‑1 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGAc                                                                                                  <  7:147891/118‑1 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTAt                                                                                         >  5:252559/1‑127 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             >  1:123154/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             >  4:345474/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             >  7:377007/1‑139 (MQ=255)

                                                      tCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACg                                                                             <  2:282975/139‑1 (MQ=255)

                                                       cACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGCCATATTCTATTTAGTTCGCACgg                                                                            >  7:317488/1‑139 (MQ=255)

                                                       cACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACgg                                                                            >  6:204473/1‑139 (MQ=255)

                                                        aCGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGt                                                                           <  3:305105/139‑1 (MQ=255)

                                                           aaCAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  5:204473/139‑1 (MQ=255)

                                                             cAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca                                                                      <  7:30678/139‑1 (MQ=255)

                                                             cAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTacaca                                                                      <  7:219737/139‑1 (MQ=255)

                                                                aTAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATTCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGTGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTg                                                                   >  6:357798/1‑139 (MQ=255)

                                                                aTAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTg                                                                   >  2:77130/1‑139 (MQ=255)

                                                                    aaGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGaa                                                               <  2:239657/139‑1 (MQ=255)

                                                                      gTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcccc                                                                                                                      <  6:391280/82‑1 (MQ=255)

                                                                      gTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGcccc                                                                                                                      >  5:391280/1‑82 (MQ=255)

                                                                      gTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAgg                                                             <  2:318738/139‑1 (MQ=255)

                                                                        ggCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  1:299051/126‑1 (MQ=255)

                                                                        ggCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        >  2:299051/1‑126 (MQ=255)

                                                                        ggCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGa                                                            >  6:191080/1‑138 (MQ=255)

                                                                         gCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        <  7:80758/125‑1 (MQ=255)

                                                                         gCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTaca                                                                        >  8:80758/1‑125 (MQ=255)

                                                                         gCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACa                                                          >  6:128265/1‑139 (MQ=255)

                                                                          cAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACAt                                                         <  2:70450/139‑1 (MQ=255)

                                                                                      tGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  7:319164/138‑1 (MQ=255)

                                                                                      tGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  7:338710/138‑1 (MQ=255)

                                                                                            ttttttCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTc                                                                                  >  3:122622/1‑97 (MQ=255)

                                                                                                    tatTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGAc                                <  5:118933/139‑1 (MQ=255)

                                                                                                          tttCTCAGACCAACTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGTCTTGCAGACATATTCTATTTAGTTCGCACGGGACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAgaga                           <  5:298926/138‑1 (MQ=255)

                                                                                                                       cTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGa                                               >  4:54944/1‑105 (MQ=255)

                                                                                                                       cTTATATGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGa                                               <  3:54944/105‑1 (MQ=255)

                                                                                                                            atGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGa                                               >  7:24502/1‑100 (MQ=255)

                                                                                                                            atGCGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGa                                               <  8:24502/100‑1 (MQ=255)

                                                                                                                               cGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              <  7:417150/98‑1 (MQ=255)

                                                                                                                               cGGCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAg                                              >  8:417150/1‑98 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGc                                                                                >  8:234006/1‑62 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                 <  8:14929/77‑1 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCg                                                                 >  7:14929/1‑77 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGATAGCGGAATGCCTGt     >  1:253484/1‑137 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt     >  4:367276/1‑137 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt     >  4:361297/1‑137 (MQ=255)

                                                                                                                                 gCTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGt     >  7:165324/1‑137 (MQ=255)

                                                                                                                                  cTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg  >  1:159148/1‑139 (MQ=255)

                                                                                                                                  cTTT‑CAAACAA‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGAGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAg  >  1:326077/1‑139 (MQ=255)

GTTCTCATCTTGTACTCTATTGAAGCAGTTTCCGTAACAACGAGATTTATAATATCACGAACAAATAGAAGTGGCAATAACTTTTTTGG‑TTTTTTTTCATATTTTTTTCTCAGACCAACTTATATGCGGCTTT‑CAAAC‑A‑AAA‑ACAAGCCCCTTAAATAGGGGCTTGCAGACATATTCTATTTAGTTCGCACGGTACACACTGCGAAGGACATTTGTTTGTGAGCGGTCTGGACCGACAGAGAAAATAGAAAGCGGAATGCCTGTCAG  >  BS168_reference_genome/4155253‑4155519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`