BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	BS168_reference_genome	165,825:1	+C	100%	noncoding (72/72 nt)	trnI‑Asn →	tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	165,825	1	.	C	100.0%	385.1 / NA	113	noncoding (72/72 nt)	trnI‑Asn	tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (58/55); total (58/55)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 117 total aligned reads displayed.

AGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATAT  >  BS168_reference_genome/165689‑165958

agTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccat                                                                                                                                   >  8:288312/1‑136 (MQ=255)

 gTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                                                                                                                  <  8:346405/139‑5 (MQ=21)

    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                                                                                                                 <  1:144042/138‑1 (MQ=21)

    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCt                                                                                                                                >  1:120453/1‑139 (MQ=21)

    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCt                                                                                                                                >  2:35849/1‑139 (MQ=21)

    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                                                                                                                  <  3:170124/136‑5 (MQ=21)

    ggACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑ccatt                                                                                                                                  >  4:170124/1‑132 (MQ=21)

        gccgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                            <  8:243282/139‑1 (MQ=21)

          cgccTATCAGATTTATTAATTCTACCATGTTTGTTTATGCAGCTTTATAT‑T‑ATTCCGCAGTAGCTCATTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTTCTTCCATa                                                                                                                          <  8:252435/139‑1 (MQ=18)

          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                                                                                                                 <  1:145612/132‑1 (MQ=18)

          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGc                                                                                                                                 >  2:145612/1‑132 (MQ=18)

          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                            >  2:326898/1‑137 (MQ=21)

          cgccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                            >  7:295132/1‑137 (MQ=21)

           gccAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAg                                                                                                                         <  6:288755/139‑1 (MQ=21)

               aGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGAGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTCCGTAGCCATTGCTTCCATAGCt                                                                                                                       >  2:266707/1‑137 (MQ=21)

                 cAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTcagc                                                                                                                   <  7:396624/139‑1 (MQ=21)

                    aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                                >  1:32428/1‑139 (MQ=21)

                    aTTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAgg                                                                                                                >  6:75782/1‑139 (MQ=21)

                         ttaATTCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGGTAACCGATCGGTCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           <  7:254811/139‑1 (MQ=21)

                              tCTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                      >  3:347296/1‑139 (MQ=21)

                               cTACCATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACtt                                                                                                      <  4:12116/138‑1 (MQ=21)

                                 aCCATGTTTGGATATGACGCTTTATAT‑TC‑TTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  4:347296/139‑1 (MQ=18)

                                  ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCTGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  1:326898/138‑1 (MQ=21)

                                  ccATGTTTGTTTATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  5:371970/138‑1 (MQ=21)

                                  ccATGTTTGTTTATGAAGCTTTAAAT‑T‑ATTCCGCAGTTGCTCAGTGGTAGAGCTATCGGATGTTAACCGATCGGGCGGAGGTTCGAATCCTACCTTCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGATCACTTCCa                                                                                                   <  6:85860/138‑1 (MQ=18)

                                       tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  4:277206/133‑1 (MQ=18)

                                       tttgtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   >  3:277206/1‑133 (MQ=18)

                                          gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           <  1:116244/122‑1 (MQ=17)

                                          gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           >  2:116244/1‑122 (MQ=17)

                                          gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           >  2:110084/1‑122 (MQ=17)

                                          gtttATGAAGCTTTATAT‑T‑ATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGGTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           <  1:110084/122‑1 (MQ=17)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGCGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTACGGAAGAGGTCAGCGGTTCGAGcc                                                                      >  7:239678/1‑139 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTca                                                                                                                     >  7:316647/1‑92 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTca                                                                                                                     <  8:316647/92‑1 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  4:289016/110‑1 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   <  8:166655/110‑1 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   >  7:166655/1‑110 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCa                                                                                                   >  3:289016/1‑110 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                         <  8:409510/120‑1 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                         >  7:409510/1‑120 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                      >  3:160918/1‑139 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                      >  5:380762/1‑139 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                      >  3:167070/1‑139 (MQ=255)

                                                                ttCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGcc                                                                      >  4:280131/1‑139 (MQ=255)

                                                                  ccGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCg                                                                    >  1:280162/1‑139 (MQ=255)

                                                                   cGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGccctg                                                                   >  3:222475/1‑137 (MQ=255)

                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                                 >  3:250704/1‑139 (MQ=255)

                                                                     cAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCtt                                                                 >  2:14950/1‑139 (MQ=255)

                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  8:159323/139‑1 (MQ=255)

                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  7:288312/139‑1 (MQ=255)

                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  8:302057/139‑1 (MQ=255)

                                                                       gTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  3:276573/139‑1 (MQ=255)

                                                                              aGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                          <  7:307641/137‑1 (MQ=255)

                                                                              aGTGGTAGAGCTATCGGCTGTTAACCGATCGGGCGTAGGGTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCt                                                          <  5:31271/137‑1 (MQ=255)

                                                                               gTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaa                                                       <  2:243956/139‑1 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                         <  8:174180/104‑1 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                         >  7:174180/1‑104 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGaa                                                                                         <  8:196809/104‑1 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                      <  8:307742/139‑1 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                      >  7:144765/1‑139 (MQ=255)

                                                                                tGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGGCAGCGGTTCGAGCCCGCTTGGAAGCTTaaa                                                      <  6:216236/139‑1 (MQ=255)

                                                                                     gagCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  1:347358/125‑1 (MQ=255)

                                                                                        cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtattat                                              >  8:249112/1‑139 (MQ=255)

                                                                                        cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtat                                                 >  6:170127/1‑136 (MQ=255)

                                                                                        cTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGtat                                                 <  5:170127/136‑1 (MQ=255)

                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTATATGTAttatta                                            >  2:317520/1‑139 (MQ=255)

                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                            >  8:315511/1‑139 (MQ=255)

                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTAttatta                                            >  4:277870/1‑139 (MQ=255)

                                                                                          aTCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCGTGGAAGCTTAAATGTAttatta                                            >  5:244075/1‑139 (MQ=255)

                                                                                             ggCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTAcc                                          <  8:295132/138‑1 (MQ=255)

                                                                                                 gTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                     <  4:113931/139‑1 (MQ=255)

                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAg                                                                                 <  1:252005/94‑1 (MQ=255)

                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAg                                                                                 >  2:252005/1‑94 (MQ=255)

                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGtt                                    >  8:51090/1‑139 (MQ=255)

                                                                                                  ttAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                     <  6:149497/138‑1 (MQ=255)

                                                                                                    acccGATCGGTCGTAGGTGCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                                   <  6:328400/136‑1 (MQ=255)

                                                                                                    aacggttCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGATTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGttt                                   <  2:418693/132‑1 (MQ=39)

                                                                                                    aaCCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCa                                                                                                                            >  3:228860/1‑49 (MQ=11)

                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTTAATGTATTATTACCAAGGTTTCTCAt                              >  6:307212/1‑138 (MQ=255)

                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                              >  2:281513/1‑138 (MQ=255)

                                                                                                         aTCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                              >  8:268012/1‑138 (MQ=255)

                                                                                                          tCGGTCGTAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCAt                              >  4:108632/1‑137 (MQ=255)

                                                                                                               cGTAGGTTCGCATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                       <  5:227581/139‑1 (MQ=255)

                                                                                                                 tAGGTTCGAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGgaga                       >  7:178751/1‑137 (MQ=255)

                                                                                                                        gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaa                                                       >  5:373954/1‑98 (MQ=255)

                                                                                                                        gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTaa                                                       <  6:373954/98‑1 (MQ=255)

                                                                                                                        gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAAGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                     <  5:312282/116‑1 (MQ=255)

                                                                                                                        gAATCCTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCAAGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGt                                     >  6:312282/1‑116 (MQ=255)

                                                                                                                           tCCTACCTGCGTAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTa           <  2:120453/139‑1 (MQ=255)

                                                                                                                             cTACCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTAtt         >  2:356231/1‑139 (MQ=255)

                                                                                                                               aCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           >  3:254867/1‑39 (MQ=255)

                                                                                                                               aCCTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTagag                                                                                                           <  4:254867/39‑1 (MQ=255)

                                                                                                                                ccTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATTGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTTAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg      <  1:266707/139‑1 (MQ=255)

                                                                                                                                ccTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg      <  6:244075/139‑1 (MQ=255)

                                                                                                                                ccTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg      <  7:249112/139‑1 (MQ=255)

                                                                                                                                ccTGCGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCg      <  7:51090/139‑1 (MQ=255)

                                                                                                                                    cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               >  2:134147/1‑78 (MQ=255)

                                                                                                                                    cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTgg                                                               <  1:134147/78‑1 (MQ=255)

                                                                                                                                    cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGatat  >  7:287304/1‑139 (MQ=255)

                                                                                                                                    cGGAGCCATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGata   <  7:315511/138‑1 (MQ=255)

AGTAGGACGCCGCCAAGCAGATTTATTAATTCTACCATGTTTGTTTATGAAGCTTTATATCTCATTCCGCAGTAGCTCAGTGGTAGAGCTATCGGCTGTTAACCGATCGGTCGTAGGTTCGAATCCTACCTGCGGAG‑CATTGCTTCCATAGCTCAGCAGGTAGAGCACTTCCATGGTAAGGAAGAGGTCAGCGGTTCGAGCCCGCTTGGAAGCTTAAATGTATTATTACCAAGGTTTCTCATAAGGAGAAAGCTTTTTTTATTGCGATAT  >  BS168_reference_genome/165689‑165958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`