Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 70,638 A→G 6.9% H34R (CAT→CGT)  spoIIE → SpoIIAA‑phosphate serine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome70,6380AG 6.9% 171.3 / 4.3 58H34R (CAT→CGT) spoIIESpoIIAA‑phosphate serine phosphatase
Reads supporting (aligned to +/- strand):  ref base A (27/27);  new base G (2/2);  total (29/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

GGCAACGAATATAACAGGTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCAGAAGAGACAA  >  BS168_reference_genome/70505‑70776
                                                                                                                                     |                                                                                                                                          
ggCAACGAATATAACAGGTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTg                                                                                                                                        >  4:312926/1‑138 (MQ=255)
     cGAATATAACAGGTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCa                                                                                                                                  <  6:197157/139‑1 (MQ=255)
                 gTGGGATATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  1:231424/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATTGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCAAGAAGATGGTGACTCATCATATGCATTAACTTTTTATCta                                                                                                                       >  6:406481/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  1:357043/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  2:27114/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  2:245533/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  5:36427/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCta                                                                                                                       >  3:239750/1‑138 (MQ=255)
                 gTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGGTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGGTTTTCta                                                                                                                       >  5:316503/1‑138 (MQ=255)
                  tGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCtata                                                                                                                     <  4:148377/139‑1 (MQ=255)
                       gaTGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGa                                                                                                                <  1:78350/139‑1 (MQ=255)
                        aggagaGTAATGGAAAAAGCAGAAAGAAGAGGGAAGCGGCCAATGGAGGGACAAGCTTTGGTAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATGATTTGCATTCACTGTTTTTCTATAAAGGa                                                                                                                <  6:36427/136‑1 (MQ=255)
                            gagGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCAt                                                                                                           <  5:163281/139‑1 (MQ=255)
                                aaTGGAAAAAGCAGAAAGATCAGTGAACGCGCCATAGGCGGGACAAGCTTTGGAAAAACTCCAATCGATTTTTAACAGGGGCACGAAGCTGGTCACTCATCATTTGCATTCACTGTTTTTCTATAAAGCATTCAtat                                                                                                         >  5:375987/1‑135 (MQ=255)
                                aaTGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTAt                                                                                                       >  6:195001/1‑139 (MQ=255)
                                         aGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATc                                                                                              >  7:208577/1‑139 (MQ=255)
                                              aaagaaGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGAtt                                                                                         >  5:321436/1‑139 (MQ=255)
                                                        gAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGa                                                                               >  8:392034/1‑139 (MQ=255)
                                                         aaCGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGa                                                                               <  2:231424/138‑1 (MQ=255)
                                                         aaCGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGAc                                                                              <  3:392617/139‑1 (MQ=255)
                                                         aaCGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGAc                                                                              <  5:195001/139‑1 (MQ=255)
                                                          aCGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACg                                                                             <  7:392034/139‑1 (MQ=255)
                                                          aCGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACg                                                                             <  1:27114/139‑1 (MQ=255)
                                                            gggCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACggg                                                                           >  5:163415/1‑139 (MQ=255)
                                                              gCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCt                                                                         <  6:385805/139‑1 (MQ=255)
                                                              gCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCt                                                                         >  2:187356/1‑139 (MQ=255)
                                                              gCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCt                                                                         <  1:245533/139‑1 (MQ=255)
                                                                   tGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGTTCACGAAGCTGGTGAATCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCat                                                                    <  4:239750/139‑1 (MQ=255)
                                                                   tGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCat                                                                    <  3:312926/139‑1 (MQ=255)
                                                                   tGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCa                                                                     >  8:225605/1‑138 (MQ=255)
                                                                   tGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCa                                                                     >  4:265078/1‑138 (MQ=255)
                                                                     gCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCatat                                                                  <  6:321436/139‑1 (MQ=255)
                                                                        ggACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCat                                                                    >  5:413773/1‑134 (MQ=255)
                                                                        ggACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCat                                                                    <  6:413773/134‑1 (MQ=255)
                                                                        ggACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCAtattat                                                               >  3:351630/1‑139 (MQ=255)
                                                                        ggACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCAtattat                                                               >  1:120532/1‑139 (MQ=255)
                                                                        ggACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCAtatta                                                                >  2:217732/1‑138 (MQ=255)
                                                                          aCAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATcc                                                             >  1:99321/1‑139 (MQ=255)
                                                                             aGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAg                                                          <  1:187356/139‑1 (MQ=255)
                                                                                   ggAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCt                                                     <  6:83280/138‑1 (MQ=255)
                                                                                   ggAAAAACTCCAATCGGTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCt                                                     <  2:371622/138‑1 (MQ=255)
                                                                                    gAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCg                                                            >  1:192682/1‑130 (MQ=255)
                                                                                    gAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCg                                                            <  2:192682/130‑1 (MQ=255)
                                                                                         aCTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCGTTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTc                                              >  7:368810/1‑139 (MQ=255)
                                                                                         aCTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCGTTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTc                                              >  7:274843/1‑139 (MQ=255)
                                                                                                 cggatattAACACGGCCACGAAGCTGGTGACTCCTCATTTGCATTCACTGTTTTGCTATAAAGGAGTCATTTAAGGTGTCATCGCATTTTTGCGTGGACGGGCTGTCATATTATCCGAGGTGCTTCCCTTCGCACTTcc                                      <  4:360742/133‑1 (MQ=255)
                                                                                                  gtattttAACAGGCGCCCGACGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTAGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTATCGCACTTCct                                     <  6:316503/136‑1 (MQ=255)
                                                                                                  gTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCct                                     <  6:158895/139‑1 (MQ=255)
                                                                                                   ttttttAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCctt                                    >  6:130270/1‑139 (MQ=255)
                                                                                                   ttttttAACAGCGGCACGAAGCTGGTGACTCATCATTTGAATTCACTGATTTTCTATAAAGGATTCATTAATGATGTCATCTGATTTTTTCTTGGACGGGCTTTCATATTATCCGAGTTGATTCCTTTCGACCTTcat                                     >  8:18857/1‑136 (MQ=255)
                                                                                                            aGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTCTCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAg                           <  5:406481/139‑1 (MQ=255)
                                                                                                               ggCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGa                        <  2:357043/139‑1 (MQ=255)
                                                                                                                    gAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGctt                   <  8:208577/139‑1 (MQ=255)
                                                                                                                          ggTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTAt              >  2:131620/1‑138 (MQ=255)
                                                                                                                              aCTCATCGTTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCagaa         <  8:274843/139‑1 (MQ=255)
                                                                                                                              aCTCATCGTTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCagaa         <  8:368810/139‑1 (MQ=255)
                                                                                                                              aCTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCagaa         <  6:163415/139‑1 (MQ=255)
                                                                                                                                tcatcaTTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCagaa         >  8:81757/1‑137 (MQ=255)
                                                                                                                                  atcatTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCg                                             >  2:367128/1‑99 (MQ=255)
                                                                                                                                  atcatTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCg                                             <  1:367128/99‑1 (MQ=255)
                                                                                                                                  atcatTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCAGAAgaga     >  3:353689/1‑139 (MQ=255)
                                                                                                                                   tcatTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAg                           <  7:72879/116‑1 (MQ=255)
                                                                                                                                   tcatTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAg                           >  8:72879/1‑116 (MQ=255)
                                                                                                                                     atTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCAGAAGAGACaa  <  4:353689/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                          
GGCAACGAATATAACAGGTGGGAGATGAGAGGAATGGAAAAAGCAGAAAGAAGAGTGAACGGGCCAATGGCGGGACAAGCTTTGGAAAAACTCCAATCGTTTTTTAACAGGGGCACGAAGCTGGTGACTCATCATTTGCATTCACTGTTTTTCTATAAAGGATTCATTTATGTTGTCATCGGATTTTTGCTTGGACGGGCTTTCATATTATCCGAGGTGCTTCCTTTCGCACTTCCTTTCTTTGGAGCGATGCTTCTTATCAGAAGAGACAA  >  BS168_reference_genome/70505‑70776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑