Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 3,439,579 T→C 5.3% S461S (TCA→TCG)  cadA ← Cd(II), Zn(II) and Co(II) exporter (ATPase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,439,5790TC 5.3% 238.6 / 3.7 75S461S (TCA→TCG) cadACd(II), Zn(II) and Co(II) exporter (ATPase)
Reads supporting (aligned to +/- strand):  ref base T (36/35);  new base C (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01

CAACGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTATCCTTTGTAAAACCTTCCGCGGGT  >  BS168_reference_genome/3439443‑3439711
                                                                                                                                        |                                                                                                                                    
cAACGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGa                                                                                                                                    <  2:372331/139‑1 (MQ=255)
  aCGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACt                                                                                                                                  >  5:293956/1‑139 (MQ=255)
  aCGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACt                                                                                                                                  >  6:231432/1‑139 (MQ=255)
   cGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACt                                                                                                                                  >  8:14107/1‑138 (MQ=255)
   cGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACt                                                                                                                                  >  2:141998/1‑138 (MQ=255)
       tACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAAtc                                                                                                                             >  5:207440/1‑139 (MQ=255)
       tACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTACATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGAAAAAATCCCGTGACCGGAGATTTCTTCGTATGACTCAAta                                                                                                                             >  7:167956/1‑138 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  1:113999/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  3:234877/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  2:312614/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  2:261255/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  2:245191/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  7:43369/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCg                                                                                                                       >  8:292366/1‑137 (MQ=255)
               ttCCAGCATTTTCATCCGGGACATCCTCAAATTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATct                                                                                                                       >  3:278251/1‑136 (MQ=255)
                  cAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCggagga                                                                                                                  <  8:4294/139‑1 (MQ=255)
                    gCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCggagga                                                                                                                  >  4:424600/1‑137 (MQ=255)
                          tCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCAtt                                                                                                           <  2:364802/138‑1 (MQ=255)
                          tCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCAtt                                                                                                           <  3:182544/138‑1 (MQ=255)
                                gggACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGt                                                                                                    <  4:250771/139‑1 (MQ=255)
                                      tCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCtt                                                                                              <  8:289250/139‑1 (MQ=255)
                                           aaTTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACg                                                                                          >  4:81507/1‑138 (MQ=255)
                                            aTTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGca                                                                                        <  7:292366/139‑1 (MQ=255)
                                                   ctctTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGAtt                                                                                  >  4:208887/1‑138 (MQ=255)
                                                        tCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAg                                                                               <  3:424600/136‑1 (MQ=255)
                                                        tCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAg                                                                               <  1:312614/136‑1 (MQ=255)
                                                           aTGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCg                                                                         >  5:8347/1‑139 (MQ=255)
                                                            tGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCgg                                                                        <  1:141998/139‑1 (MQ=255)
                                                            tGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCgg                                                                        <  7:14107/139‑1 (MQ=255)
                                                              aGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGAGTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGAt                                                                      >  6:139816/1‑139 (MQ=255)
                                                                   tttATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  <  3:418293/106‑1 (MQ=255)
                                                                   tttATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  >  4:418293/1‑106 (MQ=255)
                                                                       ttCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaat                                                             <  6:8347/139‑1 (MQ=255)
                                                                        tCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            <  1:326531/139‑1 (MQ=255)
                                                                         cccAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTCTGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            <  1:62958/138‑1 (MQ=255)
                                                                         cccAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGACAGCATTTTCCCGTAAGCTTTCCGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            >  2:208540/1‑138 (MQ=255)
                                                                         cccAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            <  4:304623/138‑1 (MQ=255)
                                                                           cAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAg                                                         >  5:227298/1‑139 (MQ=255)
                                                                           cAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAg                                                         >  7:287730/1‑139 (MQ=255)
                                                                           cAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATATTCCCGTAAGCTTTACTCACAGATTCAGCAATCGGATGCTGTGAATGAAg                                                         >  7:51286/1‑139 (MQ=255)
                                                                             ggaaGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCt                                                       <  2:213780/137‑1 (MQ=255)
                                                                             gCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGATGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCt                                                       <  2:339929/139‑1 (MQ=255)
                                                                                      tcAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATg                                              >  7:171987/1‑139 (MQ=255)
                                                                                         cttCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGAGGCTGTGAATGAAGCTCAGCGTATgccg                                           <  2:12359/138‑1 (MQ=255)
                                                                                           tCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCACGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATgccgcc                                         >  6:102837/1‑139 (MQ=255)
                                                                                            ccATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTACGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  <  1:320698/81‑1 (MQ=255)
                                                                                            ccATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTACGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  <  1:326406/81‑1 (MQ=255)
                                                                                            ccATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTACGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  >  2:320698/1‑81 (MQ=255)
                                                                                            ccATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTACGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTaa                                                                                                  >  2:326406/1‑81 (MQ=255)
                                                                                               tttACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCt                                                       <  4:319294/121‑1 (MQ=255)
                                                                                               tttACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCt                                                       >  3:319294/1‑121 (MQ=255)
                                                                                               tttACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCtt                                     >  4:121280/1‑139 (MQ=255)
                                                                                               tttACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCtt                                     >  3:123875/1‑139 (MQ=255)
                                                                                                   ccTTTGCAAATATGCCGTGAGCTTAGATTTCTTCGTATGACTCAATCTCATCGGAGGTAAGCATTTTCCCGTAATTTTTACGAACAGATTCAGCAATCTGATGCTGTGAATGAAGCTCAGCGTATTCCGCCGCTTCaa                                  <  8:167956/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGTTTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCAGTAAGCTTTACGCACAGATTGAGCAATCGGTTGCTGTGATTGAAGCTCAGCGTATGCCGCCGCTTcta                                  <  4:278251/138‑3 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  2:113999/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  7:386014/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  7:356479/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  3:283630/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  3:154996/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  4:234877/138‑1 (MQ=255)
                                                                                                   ccTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCaa                                  <  6:207440/138‑1 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAAt                                                                           <  8:5193/93‑1 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAAt                                                                           >  7:5193/1‑93 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            >  7:358557/1‑108 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGtgaatg                                                            <  8:358557/108‑1 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTaa                              >  2:373935/1‑138 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAAt                             >  7:77423/1‑139 (MQ=255)
                                                                                                       tGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAAt                             >  7:145858/1‑139 (MQ=255)
                                                                                                        gCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAAt                             <  5:231432/138‑1 (MQ=255)
                                                                                                        gCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAAt                             <  6:227298/138‑1 (MQ=255)
                                                                                                           aaaaTGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTAt                         >  3:134866/1‑139 (MQ=255)
                                                                                                            aaaTGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTATc                        <  8:77423/139‑1 (MQ=255)
                                                                                                                    tGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTATCCTTTGTaa                <  1:245191/139‑1 (MQ=255)
                                                                                                                                  ttcGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTATCCTTTGTAAAACCTTCCGCGGGt  >  6:297169/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CAACGTGTACGATTGTTCCAGCATTTTCATCCGGGACATCCTCAATTTGTTCTCTTTCCATGAGCTTTTTATTCCCAGCAAGGATCTCAGTTCCATTTACCTTTGCAAAAATGCCGTGACCGGAGATTTCTTCGTATGACTCAATCTCATCGGAGGAAAGCATTTTCCCGTAAGCTTTACGCACAGATTCAGCAATCGGATGCTGTGAATGAAGCTCAGCGTATGCCGCCGCTTCAAGTAATCTATCCTTTGTAAAACCTTCCGCGGGT  >  BS168_reference_genome/3439443‑3439711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑