Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 3,585,981 C→T 7.1% M98I (ATG→ATA)  hisB ← imidazoleglycerol‑phosphate dehydratase [Mn(II)‑dependent]

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome3,585,9810CT 7.1% 183.2 / 3.6 56M98I (ATG→ATA) hisBimidazoleglycerol‑phosphate dehydratase [Mn(II)‑dependent]
Reads supporting (aligned to +/- strand):  ref base C (16/36);  new base T (2/2);  total (18/38)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

TCGAGCGCCAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACATCGCCTTTTGCGTT  >  BS168_reference_genome/3585844‑3586115
                                                                                                                                         |                                                                                                                                      
tcgagcGCCAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTcatcca                                                                                                                                       >  8:142283/1‑139 (MQ=255)
 cgagcGCCAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCGTAAATCAATCACAACTTGAGCAAGTGCCTcatccat                                                                                                                                      >  4:384700/1‑139 (MQ=255)
  gagcGCCAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTcatccatc                                                                                                                                     <  5:168172/139‑1 (MQ=255)
  gagcGCCAGCTTCAACATAAATTCATGTACAAGCTCCGTATCGATAGTTCCGATTTTAGCAGCCTGGAATTCAGCGCGCATTTGTATGTGCGGCCGGTTGATTAAATCAATCACAACTTGATCAAGTGCCTcatccatc                                                                                                                                     <  6:25124/139‑1 (MQ=255)
       ccAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTTCTTAAATAAATCACAACTTGAGCAAGTGGCTAATACATCGGca                                                                                                                                 >  5:241908/1‑138 (MQ=255)
        cAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCCCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGcaca                                                                                                                               <  5:125873/139‑1 (MQ=255)
              ccACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACg                                                                                                                           <  7:128902/137‑1 (MQ=255)
               cacaAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGcc                                                                                                                         <  7:122115/138‑1 (MQ=255)
               cacaAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGcc                                                                                                                         <  3:107520/138‑1 (MQ=255)
                      aTTCATGTACAAGCTCCGTATCGTAAGATCCGACTTTTGCAGCCGGGAAATCAGGGCGGATTGCTAAGTGCGGCCGGTTGCTTAATTCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGGCGATCCGTa                                                                                                                 <  4:164305/139‑1 (MQ=255)
                               cAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGAt                                                                                                        <  2:145043/139‑1 (MQ=255)
                                       gTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCttttt                                                                                                >  6:40205/1‑139 (MQ=255)
                                          tCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTCTTTTa                                                                                              <  6:96919/138‑1 (MQ=255)
                                                gTTCCGACTTTAGCAGCCGGGAAATCAGCGCTCTTTTCTAAGTGAGGCAGGTTGCTTAAATCAATCACAACTTGAGCAAGTGACTCATACATCGGCACAACCGCCGATCCGTAGCGTTTGATTCCTTTTTTATAGccc                                                                                        >  7:256431/1‑138 (MQ=255)
                                                            gcagcCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGagc                                                                            <  5:4629/138‑1 (MQ=255)
                                                            gcagcCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGagc                                                                            <  5:40205/138‑1 (MQ=255)
                                                                ccGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       <  1:320029/139‑1 (MQ=255)
                                                                ccGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       <  5:315605/139‑1 (MQ=255)
                                                                ccGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       <  7:142283/139‑1 (MQ=255)
                                                                ccGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       <  2:113564/139‑1 (MQ=255)
                                                                    gAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCCCAACTTGAGCAAGTGCCTCATCTATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATAGCCCAACGCATCGCGCAGCGCCGGc                                                                   >  4:403705/1‑139 (MQ=255)
                                                                    gAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTAATCTATAGGCACAAACGCCGATCCGTAGCGTGTGGATCCTTTTTTATAGCCCCACGCTTCGAGCAGCCCCGGc                                                                   >  4:397389/1‑139 (MQ=255)
                                                                          aGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAg                                                             >  4:139277/1‑139 (MQ=255)
                                                                            cgcgcATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCtt                                                                                 <  8:350235/117‑1 (MQ=255)
                                                                            cgcgcATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCtt                                                                                 >  7:350235/1‑117 (MQ=255)
                                                                             gcgcACTGCTAAGTGCCGCCGGTTGCTTAAATCACTCACACCTTGAGCACGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCCTTTGATTCCTTTTTTATCGCCCAACGCTTCGCGCAGCGCCAGCCCTcagcag                                                          <  6:177615/139‑1 (MQ=255)
                                                                              cgcATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCTATCCGTATCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGa                                                         <  8:255721/139‑1 (MQ=255)
                                                                              cgcATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGa                                                         <  3:207645/139‑1 (MQ=255)
                                                                                    tCTAAGTGCGGCCGGGTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCg                                                    <  8:360289/138‑1 (MQ=255)
                                                                                      tAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCg                                                    >  6:358238/1‑136 (MQ=255)
                                                                                      tAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCg                                                    <  5:358238/136‑1 (MQ=255)
                                                                                          tGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCtt                                             <  6:123606/139‑1 (MQ=255)
                                                                                          tGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCtt                                             <  8:210539/139‑1 (MQ=255)
                                                                                          tGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCtt                                             <  3:139277/139‑1 (MQ=255)
                                                                                          tGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCtt                                             <  7:122974/139‑1 (MQ=255)
                                                                                           gCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       >  5:354542/1‑112 (MQ=255)
                                                                                           gCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAgcgc                                                                       <  6:354542/112‑1 (MQ=255)
                                                                                                  gTTGCTTAAATCAATCACTACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACTCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCgtg                                     <  7:78697/139‑1 (MQ=255)
                                                                                                  gTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCTATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCgtg                                     <  3:403705/139‑1 (MQ=255)
                                                                                                  gTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCTATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCgtg                                     <  3:397389/139‑1 (MQ=255)
                                                                                                  gTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCgtg                                     <  8:133013/139‑1 (MQ=255)
                                                                                                  gTTGCTTAAATCAATCACAACTTGAGCAAGGGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCgtg                                     <  8:90200/139‑1 (MQ=255)
                                                                                                    tGCTTAAATCAATCACCACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGtgtg                                   >  7:313274/1‑139 (MQ=255)
                                                                                                           atcaatcaCAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGatca                            <  6:165906/139‑1 (MQ=255)
                                                                                                                tcaCAACTTGAGCAAGTGCCTCATCCATCGGCACAAACg                                                                                                                           >  7:1087/1‑39 (MQ=255)
                                                                                                                tcaCAACTTGAGCAAGTGCCTCATCCATCGGCACAAACg                                                                                                                           <  8:1087/39‑1 (MQ=255)
                                                                                                                tcaCAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGatcatcga                        >  3:105116/1‑136 (MQ=255)
                                                                                                                tcaCAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCAAACGCTTCGAGCAGCGCCTGCCCTAATCAGATGACGATATCTTCAGTCGTGTGGTGatcaagaa                        >  5:24677/1‑134 (MQ=255)
                                                                                                                  acaACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTatat                     <  3:138856/139‑1 (MQ=255)
                                                                                                                    aaCTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGat                                                  >  4:347705/1‑108 (MQ=255)
                                                                                                                    aaCTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGat                                                  <  3:347705/108‑1 (MQ=255)
                                                                                                                         gAGGAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACAt               <  6:241908/138‑1 (MQ=255)
                                                                                                                         gAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCtt                                                                                 <  1:108918/72‑1 (MQ=255)
                                                                                                                         gAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACAt               <  8:385148/138‑1 (MQ=255)
                                                                                                                         gAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTATGATTCCTTTTTTATCGCCCAACGCtt                                                                                 >  2:108918/1‑72 (MQ=255)
                                                                                                                          aGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACATCg             <  1:368083/139‑1 (MQ=255)
                                                                                                                          aGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACATCg             >  3:162847/1‑139 (MQ=255)
                                                                                                                          aGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGATTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGGGGTGCTGATCTATATAAACATCg             >  2:46480/1‑139 (MQ=255)
                                                                                                                                tGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAg                                          >  6:119650/1‑104 (MQ=255)
                                                                                                                                tGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAg                                          <  5:119650/104‑1 (MQ=255)
                                                                                                                                      atccatcGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAAAGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATAAAAATAGCCTTTTGCGtt  >  6:304804/1‑138 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TCGAGCGCCAGCTTCCACAAAAATTCATGTACAAGCTCCGTATCGAAAGTTCCGACTTTAGCAGCCGGGAAATCAGCGCGCATTTCTAAGTGCGGCCGGTTGCTTAAATCAATCACAACTTGAGCAAGTGCCTCATCCATCGGCACAAACGCCGATCCGTAGCGTTTGATTCCTTTTTTATCGCCCAACGCTTCGAGCAGCGCCTGCCCTAAGCAGATGCCGATATCTTCAGTCGTGTGGTGATCATCTATATCAACATCGCCTTTTGCGTT  >  BS168_reference_genome/3585844‑3586115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑