BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	131	G→C	6.2%	intergenic (–/‑2847)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	131	0	G	C	6.2%	38610.4 / inf	14226	intergenic (–/‑2847)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base G (6605/5530); new base C (21/981); total (6642/7584)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 14273 total aligned reads displayed.

‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

tcaataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATg                                                                                                                                           <  2:337089‑M1/131‑1 (MQ=255)

 caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  6:80901‑M1/132‑1 (MQ=255)

 caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGa                                                                                                                                          <  4:420824‑M1/132‑1 (MQ=255)

 caataaTCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGAAGTAACTTAATAGAACGCAAATGa                                                                                                                                          <  7:379357‑M1/132‑1 (MQ=255)

       tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                   >  1:127950/1‑139 (MQ=255)

       tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                   >  8:11502/1‑139 (MQ=255)

       tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                   <  7:274759/139‑1 (MQ=255)

         caacccccAACGTTGGTCAGTTTGTTTTTTGTCTAAAATATAAATGTTTCTGTCTAACCTTATAAAATTTTCTTTTATTTAATATAAAAAAAGCGTAAAAATTAGCAGTAACTTAAATGAACGCAAATTAAAAAAGCCa                                                                                                                                 <  4:121928/133‑1 (MQ=255)

         cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTGTGGGGAACCTTATCAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTAGCAGTAACTTAATACAACGGAAATGAAAAAAGCCa                                                                                                                                 <  5:77525/139‑1 (MQ=255)

         cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  5:407200/139‑1 (MQ=255)

         cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  3:214739/139‑1 (MQ=255)

         cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTGATTTAATAGAAACATCGCGGTAAAATTACCAGTAACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                 <  7:322838/139‑1 (MQ=255)

         cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATACACCGGAAATGAAAAAAGCCa                                                                                                                                 <  1:371106/139‑1 (MQ=255)

          aTCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTAAATAGAAACATAGCGGTAAAATTCGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAc                                                                                                                                <  5:103037/139‑1 (MQ=255)

           tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               >  5:263234/1‑139 (MQ=255)

           tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               >  6:371826/1‑139 (MQ=255)

           tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               >  6:137234/1‑139 (MQ=255)

           tcctccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGAAACTTAATAGAACGGAAATGAAAAAAGCCAct                                                                                                                               <  5:208114/139‑1 (MQ=255)

           tcctccAAAGTAGCACAGTGACATTTATGTCTCAAATATTAATCTTTCTGTTCAACCTTATCAAAATTTCGTTGATTTAATAGAAACATAGCGCTAAAATTAGAACAAACTTAATAGAACGGAAATGAAAAAAGacac                                                                                                                                >  8:141858/1‑138 (MQ=255)

                 aatgTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTGTCTGGACAACATAATAAATTTTACTTTTATTTAATAGAAACATAGCGGTAAAATTACCACTAACTTAAACGACCGGAAATCAAAAAAGCCACTCTCata                                                                                                                         <  2:157196/136‑1 (MQ=255)

                 aaaGTTTGAGTGTGAGTTTTATGTCTCAAATATTAATGTTTCTGGTGAACATTATAAAATTTTCGTTGATTAAATATAAACATAGCGTTAAAATTAGCAGTAACTTAATACAACGTAAATTAAAAAAGCCACTCTCata                                                                                                                         <  6:231327/139‑1 (MQ=255)

                   aGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                                       >  1:24957/1‑139 (MQ=255)

                        gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  2:379322/1‑139 (MQ=255)

                        gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  >  4:220540/1‑139 (MQ=255)

                        gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  <  3:103621/139‑1 (MQ=255)

                        gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCGTATCAAATTTTCGTTGATTTAATAGAACCATAGAGGAAAAATTAGATGTAACTTAATAGCACGCAAATGAAAACAGCCACTCTCATATCCTAtt                                                                                                                  >  2:118976/1‑139 (MQ=255)

                        gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAAACTTATCAAATTTTCTTTGATTTAATAGAATCATAGCGGTAAAATTAGCAGTAACTTAATAGAAAGGAAATGATAAAAGCCACTCTCATATGCtttt                                                                                                                  >  8:420508/1‑139 (MQ=255)

                         gtagtgattTTTATGTTCCAAATATTATTTTTTGTTGTTAAAGTAATAAAATTTTGGTTTTTTAAATAAAAAAATAGCGTAAAAATTAGAAGTAATTTAATATAACGTAAATCAAAAAAGCCACTCTCATATGCTAtt                                                                                                                  <  2:203791/136‑1 (MQ=255)

                             tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTgctgt                                                                                                             >  5:234079/1‑135 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  5:382322/139‑1 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  7:84318/139‑1 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  4:356063/1‑138 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  4:390408/1‑138 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  1:100049/1‑138 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  7:299286/1‑138 (MQ=255)

                               agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                            >  3:420267/1‑138 (MQ=255)

                                tttttATGTCCAAAATTTTATTTTTTCTGGTGACCTTTATAAATTTTTCGTTTATTAAATACAAACATAGCGTAAAAATTAGCTTTAATTAAATCGAAGGTAATTCAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                           <  2:68877/137‑1 (MQ=255)

                                        tCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTa                                                                                                   <  3:392423/138‑1 (MQ=255)

                                         cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTAATATGCTATTGGCTACCAACCTTTAGc                                                                                                 >  3:130223/1‑139 (MQ=255)

                                          gCAAATATTAATGTTTTTGGTTAACCTTATCAAATTTTCGTTGATTTAATAGAAAGTTTGCTGTTAAATTAGCAGTAAATTAATTTAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGGTACCAACCTTTAGc                                                                                                 <  3:363479/138‑1 (MQ=255)

                                           cAAATATTAATGTTTCTGTTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAAAGGAAATTAAAAAAGACACTCTCATATGCTATTGGCTACAAACCTTTAGc                                                                                                 >  5:183165/1‑137 (MQ=255)

                                           cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACtctc                                                                                                                            >  6:169978/1‑110 (MQ=255)

                                              attttAATGTTACTGGTGAACCTTATCAAATTTTCTTTTTTTTTATACAAAAATAGCGTAAAAATTAGCAGTAACTTAATAGAACGGAAAGGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTATAGCGAGaa                                                                                            <  7:407247/136‑1 (MQ=255)

                                              atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  3:94532/139‑1 (MQ=255)

                                              atatTAATGTTTCTGGTCAACCTTATCAAATTTTCTTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTAAATAGAACGGAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  4:257644/139‑1 (MQ=255)

                                              atatTAATGTTTCGGGTCAACCTTATCAATTTTTCGTTCATTTAATATAAACATAGCGTAAAAATTAGCATTAACTTAATAGAACGTAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  4:325245/139‑1 (MQ=255)

                                                    aTGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  6:105622/138‑1 (MQ=255)

                                                       tttCTGGTTAACCTTATAAAATTTTCGTTGATTTAATAGAAACAAAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                    <  7:191346/138‑1 (MQ=255)

                                                       tttCTGGTCAACCTTATAAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTCGCATTAACTAAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                    <  7:163153/138‑1 (MQ=255)

                                                         tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACttctgtc                                                                                 >  5:354114/1‑134 (MQ=255)

                                                         tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  1:308934/1‑139 (MQ=255)

                                                         tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                 >  3:273187/1‑139 (MQ=255)

                                                         tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                  <  3:186712/138‑1 (MQ=255)

                                                         gcTGGTTAACATTATCATATTTTCGTTTATTTAATACAAACATCGCGTAAAAATTACCAGTAAATTAATAGACCGCAAATTAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                  <  1:405730/137‑1 (MQ=255)

                                                          cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCt                                                                                >  1:224151/1‑139 (MQ=255)

                                                                aaaattATAAAATTTTCGTTTATTAAAAATAAACATAGCGTTAAAATTAGCATTAAATAAATACACCGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  1:158221/135‑1 (MQ=255)

                                                                aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATCTGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          >  4:294179/1‑139 (MQ=255)

                                                                aaCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  3:65734/139‑1 (MQ=255)

                                                                 aacTTATTAAATTTTGTGTGATTAAATATAAACATAGAGTATAAATAACCTTTAACTTATTAGACCGTAATATAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  6:340332/136‑1 (MQ=255)

                                                                 aCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  7:348066/138‑1 (MQ=255)

                                                                 aCCTTATAAAATTTTCGTTGTTTTAATAGAAAAATAGCGGTAAAATTAGCAGTAAATTAATAGAACGTAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                          <  5:327657/138‑1 (MQ=255)

                                                                     tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       >  2:268136/1‑121 (MQ=255)

                                                                     tATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                     >  4:18693/1‑139 (MQ=255)

                                                                              ttCTTTTATTTAATATAAACTTAGCGTTAAATTTAGAATTAAATTAATAGAACGTAAATAAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  6:48431/138‑1 (MQ=255)

                                                                              ttCGTTTATTAAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGCAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  8:389491/138‑1 (MQ=255)

                                                                              ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                             <  8:324716/138‑1 (MQ=255)

                                                                              ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTCAATCCTGTACAGCCATACAGGACtt                                                             <  6:305292/138‑1 (MQ=255)

                                                                                               aaCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            >  4:156538/1‑90 (MQ=255)

                                                                                                aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                 <  7:248706/84‑1 (MQ=255)

                                                                                                aCATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                           >  8:176341/1‑138 (MQ=255)

                                                                                                  aTAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAAc                                        <  3:160374/139‑1 (MQ=255)

                                                                                                     gCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACtt                                      >  7:218290/1‑138 (MQ=255)

                                                                                                        gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACa                                                                   <  8:207749/106‑1 (MQ=255)

                                                                                                        gTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                  >  7:13474/1‑139 (MQ=255)

                                                                                                                aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                            <  8:352463/73‑1 (MQ=255)

                                                                                                                aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAACTAAGTTAt                          >  1:277883/1‑139 (MQ=255)

                                                                                                                aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAATTTAt                          >  5:48368/1‑139 (MQ=255)

                                                                                                                aGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAt                          >  1:338869/1‑139 (MQ=255)

                                                                                                                  cAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAttt                        >  6:198985/1‑139 (MQ=255)

                                                                                                                       aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  4:7630/1‑139 (MQ=255)

                                                                                                                       aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  1:5408/1‑139 (MQ=255)

                                                                                                                       aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACGTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                   >  5:297425/1‑139 (MQ=255)

                                                                                                                        cTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTg                  >  8:269995/1‑139 (MQ=255)

                                                                                                                           aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGttt               >  2:240381/1‑139 (MQ=255)

                                                                                                                             tAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGTCTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc             <  3:24320/139‑1 (MQ=255)

                                                                                                                               gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACt                                                                                       <  7:54789/63‑1 (MQ=255)

                                                                                                                               gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc           >  8:236535/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTc             >  3:304816/1‑134 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  5:15696/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  6:403841/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  6:263641/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  1:80285/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  1:193461/1‑139 (MQ=255)

                                                                                                                                  cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTGCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  8:297824/1‑139 (MQ=255)

                                                                                                                                   ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  7:112063/1‑138 (MQ=255)

                                                                                                                                   ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa        >  2:421123/1‑138 (MQ=255)

                                                                                                                                   ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc       <  8:70509/139‑1 (MQ=255)

                                                                                                                                   ggAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAAc       <  3:332458/139‑1 (MQ=255)

                                                                                                                                    taaaTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  2:299435/138‑1 (MQ=255)

                                                                                                                                    gAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa      <  5:127455/139‑1 (MQ=255)

‑‑‑‑‑‑‑TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGTAACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGC  >  pSIJ663_pHT315_araE_xy/1‑268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`