BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	137	A→C	5.3%	intergenic (–/‑2841)	– / → eryR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	137	0	A	C	5.3%	35075.8 / inf	13775	intergenic (–/‑2841)	–/eryR	–/–
Reads supporting (aligned to +/- strand): ref base A (6568/6170); new base C (45/974); total (6625/7150)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 14330 total aligned reads displayed.

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

tcaaTCCTCAAATGTTGTAGAGTGATTTTTATGTCCCAAATATTAATGTTTCTGGTGAACCTTATAAAATTTTCGTTTATTTAATACAAAAATAGCGTAAAAATTAGAAGT‑AAATTAATAGAACGTAAATTAAAAAAGc                                                                                                                                          <  2:66216/136‑1 (MQ=255)

tCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGc                                                                                                                                          >  5:55107/1‑139 (MQ=255)

  cagcctccACCGTGGGAGAGTGAGTTTTATGTCCCAAAAATTAATGTTTCTGGTGAACCTAATCAAATTTGCGTTGATTTAATAGAAACATAGCGGAAAAATAAGCAGT‑AACTTAATACAACGGAAATGAAAAAAGCCa                                                                                                                                        <  4:161278/136‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTTAGTTTTATGTCGAAAATATTAATGTTTCTGGTGAACCTTAAAAAATTTTCGTTTATTTAATAGAACCATAGCGGTAAAATTAGCTGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:194534/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCATT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  5:301664/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGTAAATGAAAAAAGCCa                                                                                                                                        <  3:129057/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  7:11259/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  3:241743/1‑139 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  8:328942/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATCAAAAAAGCCa                                                                                                                                        <  7:136525/139‑1 (MQ=255)

  cATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATACAACGCAAATGAAAAAAGCCa                                                                                                                                        <  6:144424/139‑1 (MQ=255)

  cATCCTCCAAAGGTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        <  6:176848/139‑1 (MQ=255)

  cATCCTACAATGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATATAAAAATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATTAAAAAAGCCa                                                                                                                                        <  7:382538/139‑1 (MQ=255)

   aTCCTCCAAAGTTGTAGATTGAGTTTTATGTCGCAAATATTAATTTTTCTGTTGAACCTTATAAAATTTTCGTTTATTTAATTGAAACATAGCGGTAAAATTAGCAGT‑AAGTTAATAGAACGTAAATTAAAAAAGCCAc                                                                                                                                       <  1:54404/139‑1 (MQ=255)

   aTCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCTTTGATTAAATAGAAACATAGCGGTAAAATTCGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCAc                                                                                                                                       <  5:103037/139‑1 (MQ=255)

    tcatcaaaaGTTGTAGAGTGATTTTTATGTCGAAAATATTATTTTTTCGGGTGAACCTTTTAAAATTTTCTTTTATTTAATAGAAAAATAGCGTTAAAATTAGCAGT‑AACTTAATAGAACGTAAATGAAAAAAGCCAct                                                                                                                                      <  4:296618/133‑1 (MQ=255)

     cctcgAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCActc                                                                                                                                     >  7:351427/1‑139 (MQ=255)

       tccAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCActct                                                                                                                                    >  4:128538/1‑138 (MQ=255)

         cAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCa                                                                                                                                  >  3:68916/1‑138 (MQ=255)

          aatgTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTGTCTGGACAACATAATAAATTTTACTTTTATTTAATAGAAACATAGCGGTAAAATTACCACT‑AACTTAAACGACCGGAAATCAAAAAAGCCACTCTCata                                                                                                                                <  2:157196/136‑1 (MQ=255)

            aGTTGGTGTTTGAGTTTTATGTAGCAAATATTAATTTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGTAAATTAAAAAAGCCACTCTCATATg                                                                                                                              <  8:70415/139‑1 (MQ=255)

                ggAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAt                                                                                                                          >  1:193393/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTAtt                                                                                                                         >  1:372051/1‑139 (MQ=255)

                 gagaGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAAACTTATCAAATTTTCTTTGATTTAATAGAATCATAGCGGTAAAATTAGCAGT‑AACTTAATAGAAAGGAAATGATAAAAGCCACTCTCATATGCtttt                                                                                                                         >  8:420508/1‑139 (MQ=255)

                      tgagtTTTATGTCGCTAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAGACATAGCGGTAAATTTAGCAGT‑AAATTAATAGAACGGAAATTAAAAAAGACAATATCATATGCTATTGGCt                                                                                                                     >  1:309321/1‑138 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  6:272486/1‑139 (MQ=255)

                      tgagtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTa                                                                                                                    >  3:157778/1‑139 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  3:185281/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  1:100049/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  6:206375/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  4:390408/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  2:41620/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  8:241044/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGA‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  3:305923/139‑1 (MQ=255)

                        agtTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGA‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAc                                                                                                                   >  6:374362/1‑138 (MQ=255)

                        agtTTTATGTCGCAAATAATAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTAAATACAAACATAGCGGTAAAATACGCAGT‑AACTTAATAGACCGCAAATCAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  2:376697/139‑1 (MQ=255)

                         gtTTTTTGTCTAAAATTTTAATGTTTCTGGTGAAAATTATAAATTTTTCGTTTATTAAATAGAAAAATTGCGGAAAAATTAGAAGT‑AAATAAATAGAACGTAAATTAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  2:119759/138‑1 (MQ=255)

                         gtTTTTTGTCACAAATATTATTGTTTCTGGTCAACCTTATAAAATTTTCTTTTATATAATATAAACATAGC‑TTAAAAATACCATTAAAATAAATACAACGTAAATGAAAAACGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  2:238729/138‑1 (MQ=255)

                         gtTTTATGTCGCAAATATTAATGTTTCTGGTTAACATTATCAAATTTTCGTTTATTTAATAGAAACATAGCGGTAAAATTAGCATA‑AACTTAATCGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  3:335468/138‑1 (MQ=255)

                                  cGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  2:202402/1‑139 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  7:411616/127‑1 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  1:339594/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  1:248514/1‑137 (MQ=255)

                                    cAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGACACTCTCATATGCTATTGGCTACCAACCTTTAGc                                                                                                        >  6:113199/1‑137 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  7:2221/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  7:302106/139‑1 (MQ=255)

                                       atatTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  1:127515/139‑1 (MQ=255)

                                           tAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGAc                                                                                               >  5:83928/1‑139 (MQ=255)

                                                tttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGACCGGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTaa                                                                                           <  4:42212/138‑1 (MQ=255)

                                                 ttCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCa                                                                                                                                        >  3:213126/1‑92 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  3:39415/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  4:76401/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  8:35790/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATcc                                                                                        >  5:2383/1‑139 (MQ=255)

                                                  tCTGGTGAACCTTATAAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATc                                                                                         <  5:127674/138‑1 (MQ=255)

                                                   cTGGTGAGCCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCAACTCTCATATGCTATTGTCTCCCACCCTTTAGCGAGAATGACTTAATCCt                                                                                       >  3:27317/1‑139 (MQ=255)

                                                   cTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATg                                                                                                                              >  8:145087/1‑100 (MQ=255)

                                                      tttaaCATTATCAAATTTGAGTTGATTTAATAGAAACATAGCGTTAAAATTCGCAGT‑AACTTAATCGAACTGAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  6:310681/136‑1 (MQ=255)

                                                      gttaaCCTTATCAATTTTTCGTTGTTTTAATAGAAACATATCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  4:325927/136‑1 (MQ=255)

                                                      gTGAACCTTATCAAATTTTCGTTGATTTAATATAAACATAGCGGTAAATTTAGCAGT‑AACTTAATATAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    <  1:277848/139‑1 (MQ=255)

                                                         aaCATTATAAAATTTTCGTTGATTAAATACAAACATAGCGCTAAAATAAGCAGT‑AAAAAAATACAACGAAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 <  3:9642/139‑1 (MQ=255)

                                                              tATCAAATTTTCGTTGATTAAATAGAAACATAGCGGTAAAATTAGCATT‑AACTTAATAGAACTGAAATTAAAAAAGCCACTCTCATATGCTATAGGCTAACAACCTATAGCGAGAATGACTAAATCCAGTACAGCCAt                                                                             >  5:185390/1‑138 (MQ=255)

                                                               ttaaaaTTTTCTTTGATTAAATAGAAACATAGCTTTAAAATTAGCTTT‑AACTTAATAGAACGTAATTGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATa                                                                            <  8:211625/135‑1 (MQ=255)

                                                                tCAATTTTTCGTTGATTTAATAGAAAAATAGCGGAAAAATTAGAACT‑AACTAAATACACCGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                           <  3:102480/138‑1 (MQ=255)

                                                                tCAAATTTTCGTTTATTTAATAGAAACATAGCGTAAAAATTAGCAGT‑AAATAAATACACCGCAAATTAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                           <  1:223323/138‑1 (MQ=255)

                                                                tCAAATTTTCGTTGATTTAATACAAACATAGCGGTAAAATTAGCAGT‑AACTTAATCTACCGGAAAGGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATAc                                                                           <  6:81693/138‑1 (MQ=255)

                                                                       ttCTTTTATTTAATAAAAACATATCGTAAAAATTACCGTT‑ACATTAATACAACGTAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  4:262007/138‑1 (MQ=255)

                                                                       ttCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  7:27745/138‑1 (MQ=255)

                                                                       ttCGTTGATTTAATAGAAACATAGCGGAAAAATTCGCAGT‑AACTAAATAGACCGCAAATGAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACtt                                                                    <  2:267569/138‑1 (MQ=255)

                                                                        tCGTTTATTTAATATAAACATAGCGGTAAAATTATCAGT‑AACTAAATAGAACGGAAATCAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTc                                                                   <  8:181034/138‑1 (MQ=255)

                                                                              aTTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTta                                                             >  6:236578/1‑138 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTAcc                                                                                                                  <  7:250665/74‑1 (MQ=255)

                                                                                         aCATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGcc                                                  >  5:209178/1‑138 (MQ=255)

                                                                                              gCGGTAAAATAAGCATA‑AAATAAATATACCGGAAATCAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                                            <  4:226275/139‑1 (MQ=255)

                                                                                               cGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTc                                            >  5:385518/1‑138 (MQ=255)

                                                                                                ggTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaa                                          <  8:300533/139‑1 (MQ=255)

                                                                                                 gTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  4:189690/1‑139 (MQ=255)

                                                                                                 gTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCaaa                                         >  5:269363/1‑139 (MQ=255)

                                                                                                    aaaTTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAAt                                        >  7:165196/1‑137 (MQ=255)

                                                                                                    aaaTTAGACTT‑AACTTAATTGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATa                                       <  7:325359/138‑1 (MQ=255)

                                                                                                          gCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCACCTTCAAATAAGTTAtt                                >  7:88081/1‑139 (MQ=255)

                                                                                                           cAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTAttt                               >  2:336883/1‑139 (MQ=255)

                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  5:412888/1‑139 (MQ=255)

                                                                                                                 aCTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCtt                          >  3:366452/1‑139 (MQ=255)

                                                                                                                     aaTAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTta                                                             >  7:58152/1‑100 (MQ=255)

                                                                                                                         gAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                  >  1:82630/1‑139 (MQ=255)

                                                                                                                          aaCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgc                  >  1:15750/1‑138 (MQ=255)

                                                                                                                           aCGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTcgcg                 >  4:35599/1‑138 (MQ=255)

                                                                                                                            cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGaa                                                                                                   <  5:359592/55‑1 (MQ=255)

                                                                                                                            cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTa                                                                                    >  6:406765/1‑70 (MQ=255)

                                                                                                                            cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  3:421829/1‑139 (MQ=255)

                                                                                                                            cGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:118206/1‑139 (MQ=255)

                                                                                                                             ggAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGaa               >  8:27339/1‑138 (MQ=255)

                                                                                                                              gAAATAAAAAACGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACa             <  6:49314/139‑1 (MQ=255)

                                                                                                                                    aaaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAg                                                                                 >  5:332247/1‑65 (MQ=255)

                                                                                                                                    aaaaaaGCAACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGATATTTGACTTGTTTTCGCGAACAAGGCt        >  5:158681/1‑138 (MQ=255)

                                                                                                                                    aaaaaaCCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCt        <  8:392411/138‑1 (MQ=255)

                                                                                                                                     aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  2:299347/139‑1 (MQ=255)

                                                                                                                                     aaaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCtta      <  6:346102/139‑1 (MQ=255)

                                                                                                                                      aaaaGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCTCGAACAAGGc         >  1:406909/1‑135 (MQ=255)

TCCATCCTCCAAAGTTGGAGAGTGAGTTTTATGTCGCAAATATTAATGTTTCTGGTGAACCTTATCAAATTTTCGTTGATTTAATAGAAACATAGCGGTAAAATTAGCAGT‑AACTTAATAGAACGGAAATGAAAAAAGCCACTCTCATATGCTATTGGCTACCAACCTTTAGCGAGAATGACTTAATCCTGTACAGCCATACAGGACTTCGACTTATAAGAGGCGCCAACTTCAAATAAGTTATTTGCCTTGTTTTCGCGAACAAGGCTTATTAG  >  pSIJ663_pHT315_araE_xy/1‑275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`