BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,729	T→G	7.0%	intergenic (+14/‑142)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,729	0	T	G	7.0%	27783.5 / inf	9375	intergenic (+14/‑142)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base T (733/7793); new base G (794/33); total (1527/7848)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9883 total aligned reads displayed.

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttt                                                                                                                                               >  5:361963/1‑139 (MQ=255)

  tttACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTAGGAGTCGC‑tgt                                                                                                                                               >  4:221849/1‑137 (MQ=255)

     aCTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑ttttt                                                                                                                                             <  5:309567/138‑1 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATGTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAAATTg                                                                                                                                     >  6:166383/1‑139 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTg                                                                                                                                     <  3:24801/139‑1 (MQ=255)

            aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTAtttt                                                                                                                                     >  6:264019/1‑138 (MQ=255)

             tCAGTTTCATCAACCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTgg                                                                                                                                    <  7:190068/139‑1 (MQ=255)

              cAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                   <  3:360398/139‑1 (MQ=255)

                     atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTAAGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                             <  2:396131/138‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:401568/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  7:135010/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  7:162053/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  2:48594/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  2:76401/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  4:153328/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  1:257223/139‑1 (MQ=255)

                      tcaAGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTAGTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAATGTTaca                                                                                                                           <  4:401317/139‑1 (MQ=255)

                      taaaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  8:268091/137‑1 (MQ=255)

                      gacaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                           <  5:115261/136‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTacac                                                                                                                          <  3:104506/138‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  7:215277/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  4:257735/139‑1 (MQ=255)

                        aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                         <  6:300536/139‑1 (MQ=255)

                                aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaaa                                                                                                                 <  7:112585/139‑1 (MQ=255)

                                    acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                             <  7:270972/139‑1 (MQ=255)

                                    acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                             <  6:197472/139‑1 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTACACGTTACTAAAaggta                                                                                                            >  8:323103/1‑137 (MQ=255)

                                     cGCCAAAGTAAACAATTTAAGTACCATTACCAATGAGCAAGTATTGTCTA‑TTTTTAATACTTATCTATTATTTAACGGGAGGAAATAAATCTATGAGGCGC‑ATTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  4:364379/139‑1 (MQ=255)

                                      ggcAAACTTAACAATTTTACTACCATTACGTTTGAGGAATGTTTGGGTATTTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                            <  1:193293/137‑1 (MQ=255)

                                      gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAAATTGGAAAGTTAAAAGTTACTAAAGGGa                                                                                                             >  1:284765/1‑137 (MQ=255)

                                       ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                          <  2:258568/139‑1 (MQ=255)

                                       ccAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATg                                                                                                          <  3:38381/139‑1 (MQ=255)

                                           aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgaga                                                                                                      <  6:84069/139‑1 (MQ=255)

                                            gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                     <  1:368162/139‑1 (MQ=255)

                                            gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGAt                                                                                                     <  8:75205/139‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                  <  6:332301/139‑1 (MQ=255)

                                               aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaa                                                                                                   <  5:216327/138‑1 (MQ=255)

                                                   aTTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGAGATAAattat                                                                                              <  1:55587/139‑1 (MQ=255)

                                                   aTTTAAGTAAGAATACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattat                                                                                              <  1:84023/139‑1 (MQ=255)

                                                    tttAATTACCATTACTTATGATCAATTATTGTCTA‑ATTTTAATTTTTATCTATTAATTAACGGGAGGAAATAAATCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattcat                                                                                             <  5:192597/139‑4 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCTAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAAAGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  2:221755/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  6:226627/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:279070/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:348800/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  7:63884/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                             <  3:165020/139‑1 (MQ=255)

                                                    tttAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAATTTAAACGTTAGTAAAAGGTATGGAGATAAattttt                                                                                             >  3:418707/1‑139 (MQ=255)

                                                    attAAGTACCATTACTTATTAGCAAGTATTGTGTA‑CTTTTAATATTTATGTTATATTTAACGGGAGGAAATAATTCTATGAGTCGA‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAtttatt                                                                                             <  1:133369/138‑1 (MQ=255)

                                                        aGTACCATTACTTATGAGCAAGGATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  2:181661/139‑1 (MQ=255)

                                                        aGTAACATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTAATATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTATAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                         <  5:245796/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  2:145086/139‑1 (MQ=255)

                                                         gTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                        <  1:341926/139‑1 (MQ=255)

                                                              aTTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTTAAATTTTCACGTTACTAAAAGGAATTGGGATAAATTATTTTatattat                                                                                    >  3:193887/1‑135 (MQ=255)

                                                               ttACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAATTTTCACCTTACTAAAGGGAATTGTGATAAATTATTATATataataa                                                                                  >  2:11378/1‑138 (MQ=255)

                                                               ttACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGTAAAGTTTAACGTTTCTTAAGGGAATGGAGAGAAATTATTTGATataataa                                                                                  >  8:238573/1‑138 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCTTTTTTTAAATTTTTAAGGTTA‑AGATTAATAAAATGAAAAGCGATAAATTATTTTATATAATAAAGACaa                                                                            >  6:133129/1‑138 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  4:18930/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  5:135456/137‑1 (MQ=255)

                                                                     aTGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                              <  3:6293/137‑1 (MQ=255)

                                                                      tGATCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                            <  7:386718/138‑1 (MQ=255)

                                                                        aGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTTTTTTTTAAGTTTGACGTGTGTAAAGGGTATTGGGCTTAATTTTTATATATAGTAATGTAAGCtt                                                                         >  1:160806/1‑139 (MQ=255)

                                                                           aaGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGTAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  8:110255/139‑1 (MQ=255)

                                                                           aaGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  7:240540/139‑1 (MQ=255)

                                                                           aaGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  4:117937/139‑1 (MQ=255)

                                                                           aaGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                      <  2:317893/139‑1 (MQ=255)

                                                                            aGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAATTTTAACGTTAATAAAGTGTATGGTGATAAAATATTATATATAATAATGAAAGCTTACa                                                                      >  5:76153/1‑138 (MQ=255)

                                                                            aGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                     <  5:164340/139‑1 (MQ=255)

                                                                             gTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  3:33226/139‑1 (MQ=255)

                                                                             gTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                    <  4:90739/139‑1 (MQ=255)

                                                                             gTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGTTAAGTTAAAAGTTTATAAAGGGTATGGTGATTAAATATTATATATACTACTGACAGCTTcc                                                                       >  2:290050/1‑136 (MQ=255)

                                                                              tATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  1:315827/139‑1 (MQ=255)

                                                                              tATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  4:53486/139‑1 (MQ=255)

                                                                              tATTGTCTA‑GTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  5:333759/139‑1 (MQ=255)

                                                                               aTTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  3:137563/138‑1 (MQ=255)

                                                                               aTTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                   <  5:276611/138‑1 (MQ=255)

                                                                                   tCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTTCACGTTTATAAATGGTATGGAGATAAATTTTTTTATATAATACGTACAGCTTGCaagaaacg                                                               >  8:1404/1‑135 (MQ=255)

                                                                                        tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                          <  7:322271/139‑1 (MQ=255)

                                                                                        tttttAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                            <  3:307185/137‑1 (MQ=255)

                                                                                         ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTTTAATTTAAACGTTACTAAAAGGAATTGTTATTAAATATTATATTTTATAATGTCAGATTACAATAACCTaaaaat                                                         >  6:10559/1‑136 (MQ=255)

                                                                                          tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                         <  8:174509/138‑1 (MQ=255)

                                                                                                 gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTATAGGGATTGGAGATAAATTATTAGATTTACTAATGACATCTTCGAATAAGCTAAAGAGGTCCCTAg                                                 <  7:298828/139‑1 (MQ=255)

                                                                                                  ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGTAAGTTACAAGTTACTAAATGGAATGGTGATAATTTATTATATATAAAAATGCAAGCTTCCAAGAAGAGTAATAAGTCCCTAgc                                                >  3:88533/1‑139 (MQ=255)

                                                                                                    aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTAAAATTAAAAGTTTATAAAAGGAATTGATTTTAAATATTTTATATAAAAAATACACCATCCAAAAACCTAAAAATGTACCAAgcgc                                              >  6:361421/1‑139 (MQ=255)

                                                                                                         ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTAc                                          <  8:423855/138‑1 (MQ=255)

                                                                                                            tttAACGGGAGGAAATAATTCTATGAGTCGA‑TTTTTTAAAATTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                       <  5:43167/138‑1 (MQ=255)

                                                                                                              tAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGGGAAATTTCAAGGTTCTAAATGGAATTGGGATAAATTATTAGATTTACAAAGGTCACCATCCCAGAAGCGAAAGAGGTGCGGAGCGCCGAAGCGGaa                                    >  8:358119/1‑139 (MQ=255)

                                                                                                               aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAATTTA‑ACGTTTATAAAAGGAATTGGGATAAATTTTTTTATATACTAATGACAGCATCCAAAAAGCTAAAGAGGTGCCAAGCGCCGACGGGGGAt                                   >  6:397282/1‑139 (MQ=255)

                                                                                                               aaCGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAAATT‑GTAATTTAAAAGTTTATAAATGGTATGGAGATTAATTATTATATTTAGAACTTACAGCTTCCAATAATCAAAATACGTCCCGAGCGGCTACggggtga                                   >  8:208538/1‑136 (MQ=255)

                                                                                                               aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTTTAAATTAAACCTTAATAAAAGGAATTTAGATTAATTATTTTTTATACTACTTCAACCGTACCAAAATCATAAGAGGTACCGAGCGCCGACGGGGAAt                                   >  8:295422/1‑139 (MQ=255)

                                                                                                                 cGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAAATTT‑TAAATATAAACCTTAGAAAAAAGTATTGAGATAAATTAATTTAAATAATAATGAAAACATCCAAAAAAATAAATAGATACCAAGAGCCAACCGGGAAttt                                 >  4:300328/1‑139 (MQ=255)

                                                                                                                         aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  8:392742‑M1/139‑8 (MQ=255)

                                                                                                                         aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGGTACACGTTACTAAAGGGAATGGAGATAAATTATAAGATATACTACTGCCAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                         <  4:32943‑M1/139‑8 (MQ=255)

                                                                                                                                   tgtttcgc‑tTTTTTTAATTTGTAATGTTAAACGTTACTAAAGGGAATGGAGATAATTTATTATATATACTACTGACAGCTTCAAAGAAGCTAACGAGGTCACTAGCGCCTACGGGGAATTTGgggtacattgaaaa                  <  2:355617‑M1/132‑15 (MQ=255)

                                                                                                                                   tGATTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  7:88964‑M1/139‑18 (MQ=255)

                                                                                                                                   tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                  <  6:424272‑M1/136‑15 (MQ=255)

                                                                                                                                    gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  1:224780‑M1/138‑18 (MQ=255)

                                                                                                                                    gAGTCGC‑TTTTTTAAATTTGGAAAGTTAAACGTTACTAAAGGTAATGGATTTAAATTATTAGATATACTACTGACAGCTTCCAAGACGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg               <  5:39753‑M1/138‑18 (MQ=255)

                                                                                                                                      gTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa             <  7:41352‑M1/138‑20 (MQ=255)

                                                                                                                                      gTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTACCTAGCGCCTAAGGGGAATTTGgggtacattgaaaaagg               >  1:10002‑M1/1‑119 (MQ=255)

                                                                                                                                       tCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaag            <  3:220181‑M1/138‑21 (MQ=255)

TGTTTACTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTA‑TTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTAT  >  pSIJ663_pHT315_araE_xy/3591‑3872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`