BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,735	A→T	9.9%	intergenic (+20/‑136)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,735	0	A	T	9.9%	28049.2 / inf	9833	intergenic (+20/‑136)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (480/8273); new base T (1037/40); total (1519/8314)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 10064 total aligned reads displayed.

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3597‑3877

      aTCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTg                                                                                                                                            <  2:171467/139‑1 (MQ=255)

      aTCAGTTTAATCAAGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTAATTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTg                                                                                                                                            <  3:162087/139‑1 (MQ=255)

       tCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTgg                                                                                                                                           <  2:45497/139‑1 (MQ=255)

       tCAGTTTCATCAACCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTgg                                                                                                                                           <  7:190068/139‑1 (MQ=255)

         aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGa                                                                                                                                          <  2:75417/138‑1 (MQ=255)

         aGTTTCAACAACAAATTAAACACGCCAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATATTTATCTATTATTTAAGGGTAGTAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGa                                                                                                                                          <  1:189132/138‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  3:411729/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  4:360357/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  5:97421/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  7:296431/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  7:321067/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  1:250056/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  1:280763/139‑1 (MQ=255)

                tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  4:110448/139‑1 (MQ=255)

                tcaAGCAATGAAACACGACAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  3:274356/139‑1 (MQ=255)

                tcaAGCAATGAAAAACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑Taca                                                                                                                                 <  8:108911/139‑1 (MQ=255)

                ccaACCAATTAAACACGCCAAAGTAAACAATTTAAGTACAATTACTTATGAGCAAGTATTGTCTATTTTTACTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGGCGCTTTTTTAAATTTGGAAAGG‑Taca                                                                                                                                 <  3:104280/138‑1 (MQ=255)

                  aaGCAATGAAACACGCCACACGAAACAATTTAAGGACCATTAATTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACg                                                                                                                               <  4:255466/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACg                                                                                                                               <  5:69557/139‑1 (MQ=255)

                  aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACg                                                                                                                               <  6:331113/139‑1 (MQ=255)

                     cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTTAATTTTTTAATT‑TTCACGtt                                                                                                                             >  8:23542/1‑138 (MQ=255)

                     cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTa                                                                                                                            <  1:310176/139‑1 (MQ=255)

                       aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACt                                                                                                                          <  8:322679/139‑1 (MQ=255)

                          acacacGGCAAACTAAACAATTTCAGTACCATTAGTTATGAGCCAGTTTTGTCTATTTTTTATAGTTATCTATTATTTAACGGGAGGATATAATTCTATG‑CGGCTCTTTTTTAAATTTGGAAAGT‑TACACGTTACTaaa                                                                                                                       <  6:35995/137‑1 (MQ=255)

                               agCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGaa                                                                                                                  <  2:252994/138‑1 (MQ=255)

                                gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTTAATTTTGAAATT‑TAAACCTTACTAAAAGGAAt                                                                                                                 >  4:397467/1‑139 (MQ=255)

                                  cAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTTAATTTTGTAAGT‑TAAACGTTACAAAAGGGaaatg                                                                                                               >  7:136411/1‑136 (MQ=255)

                                     aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGgag‑a                                                                                                           <  2:109057/139‑1 (MQ=255)

                                      gTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑At                                                                                                          <  8:422980/139‑1 (MQ=255)

                                       tAAACAATTTAAGTACCCTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATa                                                                                                         <  8:392166/139‑1 (MQ=255)

                                         aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATaaa                                                                                                       <  3:32796/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAattatt                                                                                                  <  4:216594/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAattatt                                                                                                  <  7:87136/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAattatt                                                                                                  <  3:217972/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAattatt                                                                                                  <  6:15756/139‑1 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGGTTTTTAATTTTTTTAGT‑TTGACGTTTATAAATGGAATGGAG‑ATAAattttt                                                                                                  >  6:398113/1‑139 (MQ=255)

                                              tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGGGTTTTATTTTTGAAATTGTACACATTCTTAAAGGAAAT‑GAG‑ATAAatattt                                                                                                  >  4:50472/1‑135 (MQ=255)

                                                tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTTAATTTTTAAAGT‑TTAACCGTTCTAAAAGGATAGGGG‑ATAAattatttt                                                                                                >  8:74279/1‑137 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAg                                                                                                <  5:134802/138‑1 (MQ=255)

                                                 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAg                                                                                                <  8:357620/138‑1 (MQ=255)

                                                  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGat                                                                                              <  1:133219/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGata                                                                                             <  2:349794/139‑1 (MQ=255)

                                                   gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGata                                                                                             <  6:126695/139‑1 (MQ=255)

                                                      ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGAtata                                                                                           <  1:395623/138‑1 (MQ=255)

                                                        aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTAGG‑AGTCGCGTTTTTTAATTTGGAAAGT‑TTAACGTTACTAAAGGGTATGGTG‑ATTAATTATTAGTTAtacg                                                                                         >  8:204170/1‑137 (MQ=255)

                                                            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGa                                                                                    <  2:393140/139‑1 (MQ=255)

                                                             ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGAc                                                                                   <  5:241688/139‑1 (MQ=255)

                                                              tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑ATTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGa                                                                                    >  7:60149/1‑137 (MQ=255)

                                                                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATG‑AGTCGCGTTTTTTAATTTTGAAAGT‑TAAAAGTTAATAAAAGGAATTGAG‑ATAAATTATTATATATACAAAAGACAGcg                                                                               >  8:266728/1‑138 (MQ=255)

                                                                 gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTTTATTTTTAAATT‑TAAACGTTTATTAATGGAATTGAG‑ATAAATTATTATATATAATAATGACaac                                                                                >  3:303180/1‑136 (MQ=255)

                                                                  aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCt                                                                               <  6:426833/138‑1 (MQ=255)

                                                                     aaGTATTTTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  6:295790/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATATTTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  5:161171/139‑1 (MQ=255)

                                                                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  3:134202/139‑1 (MQ=255)

                                                                     aaGTATTGTCAAATTTTAATAGTTATCTATTATTTTACGGGAGGAAATAATTCTATG‑AGTCGCTTATTTAAATTTGGAAAGT‑TACACGTTTCTATAGGGAATGGAG‑ATAAATTATTAGTTATACTACTGACAGCTTCCa                                                                           <  4:297220/139‑1 (MQ=255)

                                                                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  5:329175/139‑1 (MQ=255)

                                                                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:48536/139‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  3:379079/138‑1 (MQ=255)

                                                                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  7:214825/138‑1 (MQ=255)

                                                                          ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCaagaa                                                                       <  3:242955/138‑1 (MQ=255)

                                                                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTAAATTTTGAAAGT‑TAAAACTTAATAAAAGGAATTGTG‑ATAAATTATTATATATAATACAGACAGCTTCCAAGAAGCt                                                                    >  2:139175/1‑138 (MQ=255)

                                                                              cTATTTTTTTTTGTTATCTATTATTTAACGGGATGAAATAATTCTATG‑AGTCGCTTTTTTAATTTTGGAAAGT‑TACACGTTAATAAATGGAATTGAG‑ATAAATTATTAGATATTCTACTTTCAGCTTCCAAGAAGCTa                                                                   <  3:83398/138‑1 (MQ=255)

                                                                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTaaa                                                                 <  5:188413/137‑1 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTTTAATTGGATAGT‑TAAACGTTTCTAAATTGCATCGAGTAAAAATTTTATTATTTA‑TGCTGCCACCTACGAAGAAGCTAAagag                                                              >  4:183369/1‑139 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTTAATTTTGAAAGT‑TTCACGTTTCTAAAGGGAATGGAG‑ATAAATTTTTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              >  7:114346/1‑139 (MQ=255)

                                                                                  ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTATATTTTGGAAGT‑TTCACGTTTCTAAATGGAATGGTG‑ATAAATTATTTTATATTATACTGCCACCGTCCAACAAGCTAAagag                                                              >  8:139485/1‑139 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  7:238841/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  2:286558/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  7:164420/138‑1 (MQ=255)

                                                                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  4:325626/138‑1 (MQ=255)

                                                                                   tttAAATGTTATATATTATTTAACGGGAGGAAATATTTCTATG‑AGGCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAATTTATTAGATATACTACTGACAGCTACCAAGAAGCTAAtgag                                                              <  4:14626/138‑1 (MQ=255)

                                                                                        tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                        <  5:423429/139‑1 (MQ=255)

                                                                                           ttATCTATTATTTTAACGGTGTCAATAAATATATGTAGT‑GGTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTTTTAGATTTACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                      <  1:362592/138‑1 (MQ=255)

                                                                                           ttATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGTTTTTTAATTTGGTAAGT‑TACACGGTACTAAAGGGTATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCGAgc                                                     >  7:411730/1‑139 (MQ=255)

                                                                                             aTCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCGGTTTTTAATTTTTTAAGT‑TAAAAGATAAAAAAAGGAATTGAG‑ATAAAATATTATATATTATAAAGAAACCTTACAAAAAGCAAAAGAAGTCCCAAgacc                                                   >  6:22198/1‑136 (MQ=255)

                                                                                                     tttAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                            <  5:39183/138‑1 (MQ=255)

                                                                                                             gaggaAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                   <  5:305390‑M1/139‑3 (MQ=255)

                                                                                                              aggaAATAATTCTATG‑AGTCGTTTTTTTATATTTGGAAAGT‑TACACGTTTCTATAGGGAATGGAG‑ATAAATTATTAGTTATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGTGAATTTgggg                                  <  5:35758/139‑4 (MQ=255)

                                                                                                                   ataatTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacat                             <  5:214413‑M1/139‑9 (MQ=255)

                                                                                                                      atTCTATG‑AGTCGATTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATAAACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattga                          <  2:314449‑M1/139‑12 (MQ=255)

                                                                                                                         cTATG‑ACTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGCAATCGAG‑ATAAATTATTAGATATACTACTGCCAGCTTCCAAGAACATACAGAGGGCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                        <  8:294453‑M1/138‑14 (MQ=255)

                                                                                                                            tG‑AGTCTCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:273243‑M1/139‑18 (MQ=255)

                                                                                                                            tG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGTGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:221961‑M1/139‑18 (MQ=255)

                                                                                                                            tG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGATGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:192977‑M1/139‑18 (MQ=255)

                                                                                                                            tG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:351920‑M1/139‑18 (MQ=255)

                                                                                                                            tG‑AGTAGATTTTTTAAATTTGGAAAGT‑TACACGTTACTAAATGTAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:214583‑M1/139‑18 (MQ=255)

                                                                                                                             tgtgtctCTTTTTTAAATTTGGAAACT‑TACACGTTACTAAAGGGAATGGAC‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAATCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  6:8488‑M1/136‑18 (MQ=255)

                                                                                                                              taGTCTCTTTTTTAAATTTGGAAAGT‑TACACGTTACTCACTGTTAGGGAG‑AATATTTATTAGATATTCTACTTCAAGCTACCATGAATGTAACGAGGTCCCTAGCGCGTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAgg               <  6:260434/137‑1 (MQ=255)

                                                                                                                               aGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:383379‑M1/139‑20 (MQ=255)

                                                                                                                               aGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:347053‑M1/139‑20 (MQ=255)

                                                                                                                                gTCGCTTTTTTTAATTTGGAAAGT‑TACACGTTACTAAATGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  4:89236‑M1/138‑20 (MQ=255)

                                                                                                                                gTCGCTTTTTTAAATTTTGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTACCTAGCGCCTAAGGGGAATTTGgggtacattgaaaaagg                    >  1:10002‑M1/1‑119 (MQ=255)

                                                                                                                                gTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGTAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  1:338488‑M1/138‑20 (MQ=255)

                                                                                                                                gTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGTAATGGAG‑ATAAATTATTAGATATAATACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  3:5635‑M1/138‑20 (MQ=255)

                                                                                                                                     ttttttAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:160096‑M1/139‑26 (MQ=255)

                                                                                                                                      tttttAAATTTGGTAAGT‑TACACGTTACTAAAGGGAATTGAG‑ATAAATTATTAGATATACTACTGACTTCTTCCAAGAAGATAATGAGGTCAGTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  8:172874‑M1/138‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  1:778‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  7:26893‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:191669‑M1/137‑26 (MQ=255)

                                                                                                                                       ttttAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:115222‑M1/137‑26 (MQ=255)

CTAAAAATCAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATG‑AGTCGCTTTTTTAAATTTGGAAAGT‑TACACGTTACTAAAGGGAATGGAG‑ATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  pSIJ663_pHT315_araE_xy/3597‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`