BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	3,749	A→T	5.3%	intergenic (+34/‑122)	eryR → / → ampR	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	3,749	0	A	T	5.3%	27638.1 / 233.9	8630	intergenic (+34/‑122)	eryR/ampR	–/–
Reads supporting (aligned to +/- strand): ref base A (782/7248); new base T (554/26); total (1341/7289)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 9646 total aligned reads displayed.

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

 atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                            <  3:82293/138‑1 (MQ=255)

 atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                            <  8:20323/138‑1 (MQ=255)

 atcaAGAAATCAACCACGCCAAAGTAAACAATTTAAGTTCCATTACTTATGAGCTAGTATTGTCTATTTTTAATAGTTATCTCTTATTTTACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTa                                                                                                                                            <  2:422018/138‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  8:418262/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  3:378777/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  8:264152/139‑1 (MQ=255)

  tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  4:359225/139‑1 (MQ=255)

  tcaACCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                          <  1:356305/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACg                                                                                                                                        <  5:196103/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  3:302186/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  5:247608/139‑1 (MQ=255)

    aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  2:97179/139‑1 (MQ=255)

    aaGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATTAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                        <  1:219147/139‑1 (MQ=255)

       cAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGACTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTATTTTTTGTAATTTAAACggt                                                                                                                                      >  4:295863/1‑136 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                   <  8:73479/139‑1 (MQ=255)

         aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                   <  5:332447/139‑1 (MQ=255)

            aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                                 <  5:117706/138‑1 (MQ=255)

            aaaCACGCAAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTaa                                                                                                                                 <  5:166825/138‑1 (MQ=255)

                acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                            <  4:90131/139‑1 (MQ=255)

                 cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                           <  5:360490/139‑1 (MQ=255)

                  gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTATTTTTTTAAATTAAAAGTTTCTaaaatggat                                                                                                                          >  8:169883/1‑133 (MQ=255)

                      aaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGgag                                                                                                                      <  4:14521/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  7:238867/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  8:389670/139‑1 (MQ=255)

                         tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  3:409211/139‑1 (MQ=255)

                         acagcAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  3:32754/135‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  1:421337/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  7:378878/138‑1 (MQ=255)

                          aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  8:321610/138‑1 (MQ=255)

                           aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                   <  1:96139/137‑1 (MQ=255)

                            aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTTATTTTTTATTTTTCTCGTTAGTAAAGTGGATGGAGatatat                                                                                                                >  2:341630/1‑139 (MQ=255)

                            aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTACACGTTACTAAAGGGAATGGATATAAat                                                                                                                >  8:232940/1‑139 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  6:16050/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  1:69298/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                            <  3:54004/139‑1 (MQ=255)

                                tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTGTAAATTTACACGGTTATAAATGGCATGGAGATAAattttt                                                                                                            >  4:182417/1‑139 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGATTAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                          <  5:981/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                          <  3:27320/138‑1 (MQ=255)

                                   aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                          <  2:279197/138‑1 (MQ=255)

                                    aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                        <  5:395450/139‑1 (MQ=255)

                                        ccATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                     <  1:389101/138‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTAATTTTGTAAATTTA‑ACGTTTATAAAGGGGATGGTGATAAATTATtatatata                                                                                                     >  3:156883/1‑138 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                     <  5:71978/138‑1 (MQ=255)

                                        ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                     <  8:135954/138‑1 (MQ=255)

                                        acATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                     <  6:45631/137‑1 (MQ=255)

                                          aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAATTTAAACGTTAATAAAGGGGATTGAGAAAAAATATtatataaaat                                                                                                   >  7:117108/1‑134 (MQ=255)

                                              cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                              <  1:305518/139‑1 (MQ=255)

                                                tATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAAGTTTCAAGTTACTAAAGTGAATGGTGATAAATTATTAGATATAATACTGACa                                                                                            >  2:242817/1‑139 (MQ=255)

                                                 aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                             <  6:263326/137‑1 (MQ=255)

                                                   gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTTAAATTTCACGGTTAGAAAGGGAATGGAGATTAATTATTATATATACTAATTACacca                                                                                         >  4:215319/1‑136 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAATGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  1:332247/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  7:5654/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  8:107730/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  6:399048/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  7:296354/139‑1 (MQ=255)

                                                       aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                     <  3:216076/139‑1 (MQ=255)

                                                        aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                    <  4:110230/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                   <  5:428792/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                   <  7:268859/139‑1 (MQ=255)

                                                         gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                   <  5:139645/139‑1 (MQ=255)

                                                            ttGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaagaag                                                                                <  1:18732/139‑1 (MQ=255)

                                                              gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAAATTTGAAATTTTGACGTTTCTAAAGTGTATGGTGGGTAATTTTTATTTATAATACTGACACCTTCCaagaagg                                                                               >  6:298024/1‑137 (MQ=255)

                                                              gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTATATGATTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGc                                                                               <  2:164177/138‑1 (MQ=255)

                                                                    ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGGTTTTAATTTTGAAAGGTTGCGGGTGCTTATGGGACTTGTGATAAATTATTTTATTTATTACTGACACCTGGCAAGAACCTAAagat                                                                        >  6:130117/1‑138 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  4:325626/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  4:397694/138‑1 (MQ=255)

                                                                     tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                        <  2:69904/138‑1 (MQ=255)

                                                                      ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                                       <  7:189583/138‑1 (MQ=255)

                                                                         aTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAATTTTGTAAGTTTCAAAGTACTAAAAGGAATGGTGATAAAATATTATTTATAAAAAGTAAAGCTTACAAGAAGCTAAagagtgacc                                                                   >  6:86751/1‑134 (MQ=255)

                                                                                    ttattTAACGTGATGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGGTACACGTTACTTAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                           <  6:196196/136‑1 (MQ=255)

                                                                                    ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAACGGGAATGGAGATAAATAATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCt                                                           <  1:246743/136‑1 (MQ=255)

                                                                                      attTAACGGGCGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                       <  2:202484/138‑1 (MQ=255)

                                                                                      attTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                       <  3:351058/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTTTGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                      <  7:409979/138‑1 (MQ=255)

                                                                                       tttAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTATTTTGTAAGTTTAACGGTTCTAAAGGGTATTGTGATTAATTATTATATATTAAACGGACAGCGTCAAAGAAGCGATAGAGGTCCCGAGCGGCTACgggg                                                     >  8:354919/1‑139 (MQ=255)

                                                                                         tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACAACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                                    <  6:36216/138‑1 (MQ=255)

                                                                                                    aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGTGAATGCATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                        <  5:310666‑M1/139‑8 (MQ=255)

                                                                                                              tGAGTGTC‑TTTTTTAAATTTGTAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGATGTCCCTAGCGCCTACGGTGAATTTGgggtacattgaaaaagg                              <  7:214414‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  1:157711‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  4:253211‑M1/139‑18 (MQ=255)

                                                                                                              tGAGTCGC‑TTTTTTAAATTTCGAAAGTTACACTTTACTAAAGGGAATGGATATAAATTTTTAGATATACTAGTGACAGCTTCAAAGAAGCTAAAGAGGTCCCTAGCGCGTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAgg                         <  6:225326/139‑1 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTATATTTGGATAGTTATACGTTTCTAAAGTGAATGTAGATAATTTATTAGATTTACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCGCTAGCGCCTTCGGGGAATTTGgggtacattgaaaaagg                              <  2:305489‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTAAATTTGGAAATTTACACGTTAATAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  4:5128‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGATGGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  3:127265‑M1/138‑18 (MQ=255)

                                                                                                               gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                              <  7:83443‑M1/138‑18 (MQ=255)

                                                                                                                aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCT‑AAGAGGTCCCTAGCTCCTACGGGTAATTTGgggtactttgaaaaaggaa                            <  7:1373‑M1/138‑20 (MQ=255)

                                                                                                                aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  7:319847‑M1/139‑20 (MQ=255)

                                                                                                                aGTCGC‑TTTTTTAAATTTGGAAAGATACACGTTACTAAAGGGAATGGACATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  6:332745‑M1/139‑20 (MQ=255)

                                                                                                                 gTCGC‑TTTTTTTATTTTGGAAAGTTACACGTTACTAAAGTGAATGGATATAAATTTTTATATATACTACTGACAGCTTCCAAGATGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                            <  7:164957‑M1/138‑20 (MQ=255)

                                                                                                                 gTCGC‑TTTTTTAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTACCTAGCGCCTAAGGGGAATTTGgggtacattgaaaaagg                              >  1:10002‑M1/1‑119 (MQ=255)

                                                                                                                   cGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAAATATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagag                         <  2:130584‑M1/139‑23 (MQ=255)

                                                                                                                       ttttttAAATTTGGAAAGTTACACGTTACTAACGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  8:293016‑M1/139‑26 (MQ=255)

                                                                                                                        tttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  5:221360‑M1/138‑26 (MQ=255)

                                                                                                                         ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                      <  1:27511‑M1/137‑26 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                 <  3:188445‑M1/138‑31 (MQ=255)

                                                                                                                             aaaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta                 <  1:132949‑M1/138‑31 (MQ=255)

                                                                                                                              aaTTTGGAAAGTTACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt               <  3:239860‑M1/139‑33 (MQ=255)

                                                                                                                              aaTTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt               <  5:279156‑M1/139‑33 (MQ=255)

                                                                                                                              aaTTTGCAAACTTACACTTTACAAAAGGGAATGTAGCTAAATTATTAGATATACTACTGAAAGCATCCAAGAAGCTAAAGCGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaacgaagagtatgagtctt               <  7:343553‑M1/139‑33 (MQ=255)

                                                                                                                                        gTTACACCTTACTACAGGGAATGGAGATAAATTAATCGATATACTACTGACAGCTTCCAAGAACCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttcc     <  2:384053‑M1/139‑43 (MQ=255)

CATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTT  >  pSIJ663_pHT315_araE_xy/3611‑3887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`