BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	4,648	T→A	10.8%	Y260N (TAC→AAC) ‡	ampR →

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	4,648	0	T	A	10.8%	20008.7 / inf	7358	Y260N (TAC→AAC) ‡	ampR
Reads supporting (aligned to +/- strand): ref base T (4923/1518); new base A (15/844); total (4950/2408)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 7444 total aligned reads displayed.

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  pSIJ663_pHT315_araE_xy/4511‑4786

ttGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTa                                                                                                                                           >  1:418149/1‑139 (MQ=255)

ttGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAGAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCCCTGGGGCCCGATGGTCAGCCCTCCCGTATCGTAGTTATCTa                                                                                                                                           >  5:142808/1‑139 (MQ=255)

  gCAGGACCACTTCGGCGCTCGGCCCTGCCGGCTGGGTGGTTTATTGCTGATAAATCGGGCGCGGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACGGGGGCCAGAGGGTAAGCCCTCCCGTATCGTCGTTATCAaca                                                                                                                                         <  8:74358/139‑1 (MQ=255)

  cccgcaCCACTTCGGCGCGCGGCCCTGCGGGGGGGCTGTTTTATTGCTTATAATTCTGGCGCGGGGGTGCGGGGGTCTCGGGGTATCATTGCACCACGGGGGCCAGTTGGTAACCCCTCCCGTATCGTAGTTATCTcca                                                                                                                                         <  6:110858/134‑1 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  6:394902/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  4:224139/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                                        >  8:289003/1‑139 (MQ=255)

   cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTGTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTCTCGTAGTTATCTacac                                                                                                                                        >  3:207273/1‑139 (MQ=255)

         cACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  3:49387/1‑139 (MQ=255)

           cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                                                  >  7:86169/1‑137 (MQ=255)

              cTGCGCGCGGCCCTTCCGGCTGGCGGGTTTTTTGCTGATAAATCTGGCGCCGGTGCGCGGGGGTCGCGCGGTATCATTGCCGCCCTGGGGCCTGATGGTAAGCCCTCCCTTATCGTAGTTATCAACACGACGGGGAGt                                                                                                                              <  1:6301/138‑1 (MQ=255)

                gcgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCAGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCTGATGGTAAGCCCTCCCGAATCGTAGTTATCTACACGACGGGGAGGCAg                                                                                                                           >  1:85985/1‑139 (MQ=255)

                 cgcgcgGCCCTTCCGGATGTGTGTTTTTTTGCTTATAAATCGGCCCGTGGTGTGGGGGGGTGTGGCGTTATCTTTCCAGCAGTGGGGCCAGCTGTTACGCCCTCCCGTTGCTTTTTTTTCTACACGACGGGGAGTCAgg                                                                                                                          <  8:208323/139‑1 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  4:337363/1‑139 (MQ=255)

                 cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                                          >  2:2418/1‑139 (MQ=255)

                  gcggggCCCTGCCGGGGGGCTGGTTTTTTGGTTATAATTCTGGCGCGGGGGTGGGGGGGCCTCGCGGTATCATTGCCCCACGGGGGCATTATGGTACCCCCTCCCGTATGGTAGTAATCTCCACGACGGGGAGTCAGGc                                                                                                                         <  4:401192/135‑1 (MQ=255)

                  gcgcGGCCCTTCCGGGGGGGTGGTTTATTGCTGATAAAGCTGCAGCCGGTGCGCGGGGGTCGCGCGGAATCTTTGCAGCCCGGGGGCCAGATGGAACGCCCTCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGc                                                                                                                         <  8:400385/136‑1 (MQ=255)

                    tCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCaa                                                                                                                       >  2:170548/1‑139 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCTTTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTTTCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  2:113041/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTTTCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  7:2367/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGTCGGGGAGTCAGGCAAc                                                                                                                      >  4:43825/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  8:177610/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  6:220889/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  3:341747/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATATACACGACGGGGATTCAGGCAAc                                                                                                                      >  8:370751/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCACTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  2:43478/1‑138 (MQ=255)

                      ggCCCTTCCGGCTGGCTGGTTTATTGATGATAAATCTGGAGCCGGTGAGCGTGGGTCTCTCGGTATCATTGCAGAACTGGGGACAGATGGTAAGCACTCCAGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                                                      >  5:330226/1‑138 (MQ=255)

                      ggCCCTGCCGGGGGGCGGGTTTATTGCGCAAAAAGGGGGCGCCGGTGCGCGGGGGGCGCGGGGAATCATGCCACCACGGGGGCCAGAGGGTACGCCCCCCCGTAGCGTAGTTATCACCACGACGGGGAGTCAGGCAACt                                                                                                                     <  4:175781/139‑1 (MQ=255)

                             ccGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAt                                                                                                               >  3:101411/1‑138 (MQ=255)

                              ggggtggctgtTTTATGTCTTATACATCGGGCGCCGGTGAGCGGGGGTCGCGCGGTATCATGCCCCCCCGGGGGCCCTATGGTACGCCCGCCCGTATCGTAGTAATCACCACGACGGGGAGTCAGGCAACTATGGATGa                                                                                                             <  8:259887/135‑1 (MQ=255)

                               ggctgggtGGTTTTTTGCTCAAAAATCTCGCCCGGGGGCGGGGGGGCCCCGCGTTATATTTGCCCCCCGGGGCCCATATGCTAACCCCCCCCGAATCGTATTAATAAACACGACGGGGAGTCAGGCAACTATGGATGaa                                                                                                            <  6:427750/139‑1 (MQ=255)

                                  tggctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACg                                                                                                          >  8:143129/1‑138 (MQ=255)

                                   ggggtgTTTATGCCTCATAATTCTGGCGCCGGTGCGCGGGGGCCGCGCGTACCCATGCCAGCCCGGGCCCCAGATGGAACCCCCCCCCGTATCCTAGTTACCAACACGACGGGGAGTCAGGCAACTATGGATGAACGaa                                                                                                        <  4:80309/134‑1 (MQ=255)

                                    gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  4:30193/1‑139 (MQ=255)

                                     ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  2:68637/1‑138 (MQ=255)

                                     ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                                       >  2:386253/1‑138 (MQ=255)

                                                 tGATAAAGCGGGCGCGGGTGAGCGGGGGGCGCGCGGTATCATTCCCGCCCGGGGGCCAGTTGGTAACCCCACCCGTCGCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          <  4:142813/139‑1 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  3:274749/1‑138 (MQ=255)

                                                  gATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACAAGACGCGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATctct                                                                                          >  1:27912/1‑138 (MQ=255)

                                                   aTAATTCTGGCGCCGGTGCGCGGGGGGCGCGCGGTATCTTTGCACCACGGGGCCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          <  3:400885/137‑1 (MQ=255)

                                                   aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                                          >  5:251105/1‑137 (MQ=255)

                                                      actCTGGAGCGGGGGCGCGGGGGTCGCGCGGAATCATGGCCGCACGGGGGCCAGATGGTAAGCCCTCCCGAATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                     <  7:149019/137‑1 (MQ=255)

                                                      aaTCTGGCGCCGGTGTGCGTGGGTCGCGCGTTATCATTGCAGCACTGGGGCCATATGGAAAGCCCTCCCTTATCGTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGAt                                                                                     <  8:339390/139‑1 (MQ=255)

                                                             aGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGcc                                                                              >  7:208805/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  5:43577/1‑139 (MQ=255)

                                                                  gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACt                                                                         >  1:37351/1‑139 (MQ=255)

                                                                      gCGGGGGTCGCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCACCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  6:145566/139‑1 (MQ=255)

                                                                               tcGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCCCTGATTAAGCATTgg                                                            >  5:217544/1‑139 (MQ=255)

                                                                                cgctGTATAATTGCAGCAGTGGGGCCAGATGTAAACCCCTCCGGTAGCTTTCTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCGCACTGATTAAGCATTGGt                                                           <  5:164507/139‑1 (MQ=255)

                                                                                 gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCACTgtct                                                          >  5:77367/1‑136 (MQ=255)

                                                                                   ggTATCATTGCTGGAGGGGGGCCAGAGGGTAAGCCCTCCCGTTTGGTTTTTATGTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAc                                                        <  7:38931/139‑1 (MQ=255)

                                                                                      aTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     >  1:226184/1‑139 (MQ=255)

                                                                                       tctttGCAGCAGTGGGGCCAGATGGTAAGCCCCCCGGTATCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     <  5:395704/135‑1 (MQ=255)

                                                                                           tGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                >  1:126038/1‑139 (MQ=255)

                                                                                           ggcagccCGGGGGCCCGATGGGACGCCCTCCCGGATCGTTGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGAGCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                                                <  2:302710/138‑1 (MQ=255)

                                                                                             ccgcaCGGGGGCCATATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               <  8:31360/136‑1 (MQ=255)

                                                                                             cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               >  5:364915/1‑138 (MQ=255)

                                                                                             cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                                               >  5:292887/1‑138 (MQ=255)

                                                                                             cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCAGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTAAGAcc                                               >  7:307133/1‑138 (MQ=255)

                                                                                               gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                                            >  3:128857/1‑139 (MQ=255)

                                                                                                  ctccggCCATATGCTAAGCCATCCCCTATCGTAGTTATCTACACGACGGGGACTCAGCCACCAATGCATGAACGAAATACACAGATCGCTGAGATACCTACCACACAGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  8:4938/5‑138 (MQ=255)

                                                                                                   tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  3:83640/1‑139 (MQ=255)

                                                                                                   gggggCCAGATGGTAACCCCCCCCTTAGCGTATTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  7:284782/138‑1 (MQ=255)

                                                                                                   ggggcCCAGATGGCAAGCCCGCCGGTATCTTTTTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  1:308614/138‑1 (MQ=255)

                                                                                                   ggggcCCAGAGGGTAACCCCGCCCGTATCGTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        <  1:249237/138‑1 (MQ=255)

                                                                                                    ggggCCATTTGTTAAGCCCTCCATTTCGGTTTTTATCTACACTACGGGTAGTCAGGCAACTATTGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAATCATTGGTAACTGTCAGACCAAGTTTa                                        <  3:372400/138‑1 (MQ=255)

                                                                                                     gggCCATATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      <  7:11817/139‑1 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATATGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  2:338498/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                                        >  6:169517/1‑137 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTAc                                       >  2:208713/1‑138 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  8:113446/1‑139 (MQ=255)

                                                                                                     gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  3:184653/1‑139 (MQ=255)

                                                                                                         ccgATGGTAACCCCAGCCTTATCGTAGTTAACTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCata                                  <  7:422133/137‑1 (MQ=255)

                                                                                                          agctGGTACGCCCCCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  5:112168/136‑1 (MQ=255)

                                                                                                            aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata                                >  1:338905/1‑138 (MQ=255)

                                                                                                            aTGGCAACCCCCCCCGTAGAGAATTTTACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat                                 <  4:110897/137‑1 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     >  6:82126/1‑114 (MQ=255)

                                                                                                               gTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACt                            >  2:14096/1‑139 (MQ=255)

                                                                                                                      ctctCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACAAAGTTTACTCATATATACTTTAgatt                     >  4:2007/1‑139 (MQ=255)

                                                                                                                      cTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt                                      >  1:100005/1‑122 (MQ=255)

                                                                                                                       tCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTTTACTCATATATACTTTAgatt                     >  1:197457/1‑138 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                                                     <  7:261691/105‑1 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                                          >  2:272867/1‑116 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAgattga                   >  3:307731/1‑139 (MQ=255)

                                                                                                                        cccGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAGTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATAATTTAgattga                   >  7:363381/1‑139 (MQ=255)

                                                                                                                             aTCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTa               >  3:15821/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTTTCTACACGACGGGTAGTCAGGCAACGATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAAATGTCAGACCTAGTTTACTCATATATACTTTAGATTGATTTag              >  3:23025/1‑137 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  7:179903/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  1:160651/1‑138 (MQ=255)

                                                                                                                              tCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTaa              >  6:304138/1‑138 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  4:369685/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  6:368186/1‑139 (MQ=255)

                                                                                                                                 tAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACt          >  6:339273/1‑139 (MQ=255)

                                                                                                                                    ttATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     <  5:191401/77‑1 (MQ=255)

                                                                                                                                    ttATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                                                     >  6:191401/1‑77 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAAcctgtct     >  7:336294/1‑133 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  6:263652/1‑139 (MQ=255)

                                                                                                                                      aTCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtt     >  7:62843/1‑139 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   >  8:235427/1‑138 (MQ=255)

                                                                                                                                         tACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAACAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCAtttt   <  4:263581/138‑1 (MQ=255)

TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTT  >  pSIJ663_pHT315_araE_xy/4511‑4786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`