BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	pSIJ663_pHT315_araE_xy	5,464	T→G	5.8%	intergenic (+733/–)	ampR → / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	pSIJ663_pHT315_araE_xy	5,464	0	T	G	5.8%	24935.9 / 274.5	11583	intergenic (+733/–)	ampR/–	–/–
Reads supporting (aligned to +/- strand): ref base T (5034/5549); new base G (322/555); total (5462/6121)
Fisher's exact test for biased strand distribution p-value = 5.12e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 11651 total aligned reads displayed.

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCC  >  pSIJ663_pHT315_araE_xy/5326‑5598

   gCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                     <  7:209790/139‑1 (MQ=255)

   gCGGACAGGTAGCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGt                                                                                                                                     <  6:296461/139‑1 (MQ=255)

      gACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGAATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAg                                                                                                                                  >  7:420987/1‑139 (MQ=255)

      accgggTCTCCGGTACGCGGCAGGGACGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAg                                                                                                                                  <  1:196922/135‑1 (MQ=255)

       aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                 >  5:380091/1‑139 (MQ=255)

           gTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGAGGGTCAgggggg                                                                                                                             >  6:246660/1‑139 (MQ=255)

           gTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgggggg                                                                                                                             <  1:72376/139‑1 (MQ=255)

             agccGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  3:306102/137‑1 (MQ=255)

             acccGGAACGCGGCCGGGGCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  1:391108/137‑1 (MQ=255)

              tCCGGTACGGGGGAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCGAGGGGGATACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCTGCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  2:264168/138‑1 (MQ=255)

              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTGCAGGGGGAAACGGCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  6:100970/138‑1 (MQ=255)

               ccGGTAAGCGGCAGGGTCGGAACAGGACAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                         <  6:360333/139‑1 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCAGGTCTGGGGGGCGGCGcc                                                                                                                      >  5:25105/1‑138 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  3:184242/133‑1 (MQ=255)

                   tAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGcc                                                                                                                      >  2:101465/1‑138 (MQ=255)

                     cgCGGCAGGGTCTGAACGGGACAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTCTCGCGTTTCGCCCCCTCTGACTTGAGCGGCGATATTTGGTCTTCGAGTCAGGGTGGCGGAGCCTag                                                                                                                   >  8:210794/2‑138 (MQ=255)

                     aGCGGCAGGGTCGGAACCGGAGAGCGCACTAGGGAGCTTCCAGGGGGAAACGCCTGGTAGCTTTATAGTCCTGTCGGGTTTCGCCACCTATGACTTGATCGTCGATTTTTGTGATGCTCTTCAGGGGGGCGGAGCCTa                                                                                                                    <  6:410342/138‑1 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGa                                                                                                                         <  8:362052/133‑1 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCGTAt                                                                                                                   >  3:35303/1‑139 (MQ=255)

                     aGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                   >  6:326592/1‑139 (MQ=255)

                          cAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaa                                                                                                               >  4:409433/1‑138 (MQ=255)

                             ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGCGTTTCGCCGCCTCTGACTTGAGCGTCGAGTGTTGTGCTGCAGGACAGGGGGGCGGAGGCTTTGGaaaagc                                                                                                           >  1:127665/1‑137 (MQ=255)

                               tCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGGCTAGTTTTGTGAAGCTCGTTCCGGGGGCCGAGGCAATCGaaaaaacc                                                                                                         >  6:20848/1‑136 (MQ=255)

                                 ggAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGGGGAGCCTATGGAAAAACGc                                                                                                         >  6:157174/1‑137 (MQ=255)

                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                <  3:77322/139‑1 (MQ=255)

                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                >  6:387002/1‑139 (MQ=255)

                                          gagCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                              <  6:69885/139‑1 (MQ=255)

                                           agCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCAGCAACCCGGc                                                                                             >  4:8386/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCTGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  1:32205/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCTCCACCACTGACTTGAGCGTCGATTTTTTTGATTCTCGTCAGGGGGGAGGAGCCTATTGAAAAACGCCACCAACCCGGCCt                                                                                           >  3:159858/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGGTGGTCGTCAGGGGGGCGGGGCCGTTGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  3:49470/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTATCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAAGCGGcca                                                                                           >  5:167327/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCg                                                                                                                           <  8:303690/107‑1 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCCGCAACGCGGCCt                                                                                           >  6:184642/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTAt                                                                                                                   <  2:178978/115‑1 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                              <  2:137393/120‑1 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  4:195922/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTTGTTCTCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCAGGAACGCGGccg                                                                                           >  5:117910/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGAGTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  5:784/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  5:191703/1‑139 (MQ=255)

                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTAGCCAACTCTGAATTGAGCGTCGATTTTTTTGATTATAGTGAGGTGGGCGGAGCCTTTGGAAAAAAGCAAGCAACGCGGccg                                                                                           >  3:277939/1‑138 (MQ=255)

                                             cgcACGAGGGAGCTTACAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                           >  1:25910/1‑139 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCGTGGGGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTGGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                      <  4:351931/139‑1 (MQ=255)

                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                      >  5:143009/1‑139 (MQ=255)

                                                      gAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                <  2:43789/125‑1 (MQ=255)

                                                       aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACCCCCGCAACCCGGCCTTTTTACgggt                                                                                 >  6:133787/1‑137 (MQ=255)

                                                       aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGtt                                                                                 >  4:169076/1‑139 (MQ=255)

                                                         ctgccAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                               <  5:219089/136‑1 (MQ=255)

                                                         cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCTCGTCAgg                                                                                                                                 >  2:28978/1‑89 (MQ=255)

                                                                  ggAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACCCGGCCATTTTAAGGTTActggcctttt                                                                      >  2:329986/1‑139 (MQ=255)

                                                                    aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCGTTTTACGGTTCctggccttttgc                                                                    >  5:404626/1‑139 (MQ=255)

                                                                       cGCCTGGTATCTTTATAGGCCTGTAGGTTTTCGCCACCGCTGAGTTGAGCGAGGATTTTTGTGATGCTCGTCAGGGGGGCTGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctgt                                                                 <  7:6431/139‑2 (MQ=255)

                                                                           tGGTATCTTTTTCGTCGTGTCGGGTTTCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGCTCGTCTGGGGGGGGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                             <  7:297811/139‑1 (MQ=255)

                                                                           tGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                             >  7:270147/1‑139 (MQ=255)

                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCGAAGGAAAAACGCCAGCAACCCGGCCGTTTTACGGTTCCtggccttttgctggccgtttgcaac                                                      >  6:94840/1‑136 (MQ=255)

                                                                                    tatcGTCCTGGCGGGTTTCGCCACCTCTGACTTGCGCGTCGATGTTTGTGCGGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCGGGCCTTTTGCTCAC                                                     <  3:210107‑M1/138‑1 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGc                                                                                                         <  8:243600/86‑1 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                        <  8:394215/135‑1 (MQ=255)

                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACA                                                    >  7:355684‑M1/1‑139 (MQ=255)

                                                                                    tataGTCCTGGCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                        <  3:251382/135‑1 (MQ=255)

                                                                                      taGTCCTGTCGGGTTTCGCCACCTCTGCCGTGAGGGTCGATTTTGGTGATGCGCGTGAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATG                                                  <  7:181618‑M1/139‑1 (MQ=255)

                                                                                        ggcctgTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT                                                 <  3:4070‑M1/136‑1 (MQ=255)

                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTg                                                >  3:130428‑M1/1‑138 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTCTCGTCAGGGGGGCGGCGCCTATTGAAAAAAGCCCCCAACGCGGCCTTTTTACGGTTCCTGGCCGTTTTCTGGCCTTTTGCTGACATGTgcgg                                             >  8:45587‑M1/1‑135 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                             <  1:49217‑M1/139‑5 (MQ=255)

                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcgg                                             >  3:102767‑M1/1‑135 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAAGGTTCCTGGCCTTTTGCTTGCCATTTGCTCACATGGgcggc                                            >  4:110145‑M1/1‑134 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATTCTCGGCAGGGGGGCGGCGCCGATTGAAAAAAGACAGCAACGCCGGCATTTTTCGGTTCCTGGCCTTTTGCTTGCCGTTTGCTCACCTGttgggc                                            >  2:36815/1‑135 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAgg                                                                                                                                 <  1:231755/54‑1 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggc                                            >  8:274631‑M1/1‑134 (MQ=255)

                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggc                                            >  3:382436‑M1/1‑134 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGc                                                                                             >  7:327132/1‑88 (MQ=255)

                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTAACGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGGTCCTGGCCGTTTGCGGGCCTTTTGCTCACATGTgcctgt                                           >  7:125182‑M1/1‑132 (MQ=255)

                                                                                                   tttCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGGCTTTTTACGGTTGCTGGCCGTTTGGTGGGCTTTTGCTCACATGTgcggcgggggtt                                     >  2:71592‑M1/1‑127 (MQ=255)

                                                                                                     tCGCCACCTCTGACTTGAGCGGCGATTTTTGGGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                    <  8:69597‑M1/138‑14 (MQ=255)

                                                                                                      cGCCACCTCGGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttgg                                   <  4:54170‑M1/138‑15 (MQ=255)

                                                                                                        ccACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcgtggcgg                                >  7:387503‑M1/1‑122 (MQ=255)

                                                                                                         cACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGGCAGGGGGGCGGATCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggc                               <  3:330407‑M1/139‑19 (MQ=255)

                                                                                                         cACCTCTGACTTGAGCGTCGATTTTTCTGATGCTCGTAAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGAgcggcggggcttggcggc                               <  8:119630‑M1/139‑19 (MQ=255)

                                                                                                          aCCTCTTACTTGATCGTGGATTTTTTGGATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                              <  1:355583‑M1/139‑20 (MQ=255)

                                                                                                          aCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggct                              <  4:234089‑M1/139‑20 (MQ=255)

                                                                                                                 actggcgcggcgatttgtGTGAGGCGCGTCCCGGGGGCGGAGCCTATGGAACACCGCCAGCCACGCGGCCTGTTTACGGTTCCTGGCCTTTTGCT‑GCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  4:267744‑M1/131‑26 (MQ=255)

                                                                                                                 aCTTGAGGGTCGATTTTTGTGATGCTCGGCTGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  4:139802‑M1/138‑26 (MQ=255)

                                                                                                                 aCTTGAGCGTCGTTTTTTTGGATGCGGGGGCGGGGGGCGTAGCCTATGCAAAAACGACAGCAACGAGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctttttgta                       <  1:160381‑M1/139‑27 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGTTGCTCGTCAGGGGGGGGGTGCCTTTGGAAAAACGCCAGGATCGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  7:90386‑M1/138‑26 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTGGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  5:428206‑M1/138‑26 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                       >  2:23408‑M1/1‑113 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                       <  5:355953‑M1/139‑27 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  1:284551‑M1/138‑26 (MQ=255)

                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGCGCGTCAGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgta                       <  4:323520‑M1/139‑27 (MQ=255)

                                                                                                                 aCTTGAGCGTCGAGTTTTGTGATGCTCGTCTGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgt                        <  5:292157‑M1/138‑26 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGc                                                                                             <  8:177442/64‑1 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggct                  >  4:379198‑M1/1‑108 (MQ=255)

                                                                                                                      aGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggctta                            >  1:100353‑M1/1‑108 (MQ=255)

                                                                                                                        cGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttg                                    <  4:300644‑M1/119‑14 (MQ=255)

                                                                                                                           cGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTggccttttgctggccttttgct                                                        >  7:152166/1‑96 (MQ=255)

                                                                                                                            tatTTTGGTGATGGTCGTTCGGGGGGCGGTGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca            <  5:32332‑M1/138‑38 (MQ=255)

                                                                                                                            gATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca            <  8:147246‑M1/139‑38 (MQ=255)

                                                                                                                            gATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca            <  7:23904‑M1/139‑38 (MQ=255)

                                                                                                                            gATTTTTGTGATGCTCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTgcggcggggcttggcggcttattgtatggctatgaca            <  8:333369‑M1/139‑38 (MQ=255)

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCC  >  pSIJ663_pHT315_araE_xy/5326‑5598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`