Predicted mutation
evidence seq id position mutation freq annotation gene description
RA BS168_reference_genome 78,236 A→T 100% N418I (AAT→ATT)  ftsH → ATP‑dependent cytoplasmic membrane protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome78,2360AT100.0% 165.5 / NA 50N418I (AAT→ATT) ftsHATP‑dependent cytoplasmic membrane protease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (20/30);  total (20/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGCTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCAATATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGGTTATGCTGTTATGCTGCCAAGAGAAGA  >  BS168_reference_genome/78115‑78368
                                                                                                                         |                                                                                                                                    
gcgcTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACg                                                                                                                     <  2:177278/139‑1 (MQ=255)
 cgcTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACg                                                                                                                     >  5:146746/1‑138 (MQ=255)
 cgcTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACg                                                                                                                     >  7:304561/1‑138 (MQ=255)
 cgcTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACg                                                                                                                     >  7:67121/1‑138 (MQ=255)
   cTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGGTATCTCCAAGAAAGAACGCATTATCGGGGCTTATCAGGAAg                                                                                                                  >  2:118032/1‑139 (MQ=255)
      gTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACTGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGc                                                                                                                <  7:318411/138‑1 (MQ=255)
      gTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCg                                                                                                               >  3:378134/1‑139 (MQ=255)
          cGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGaca                                                                                                           <  1:251680/139‑1 (MQ=255)
          cGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGaca                                                                                                           <  8:304561/139‑1 (MQ=255)
           ggCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGacac                                                                                                          <  6:417065/139‑1 (MQ=255)
            gCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGacaca                                                                                                         <  4:424840/139‑1 (MQ=255)
             cTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCAAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGacacac                                                                                                        >  7:69329/1‑139 (MQ=255)
                   aaaaCAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTAt                                                                                                  <  8:115553/139‑1 (MQ=255)
                        aaGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGtc                                                                                             <  8:69329/139‑1 (MQ=255)
                                  tCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGa                                                                                   <  5:429124/139‑1 (MQ=255)
                                  tCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGa                                                                                   <  6:269490/139‑1 (MQ=255)
                                    gATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATg                                                                                 >  7:75585/1‑139 (MQ=255)
                                    gATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGAACCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGAt                                                                                  >  5:402809/1‑138 (MQ=255)
                                     aTGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGacac                                                                                                          >  8:233259/1‑113 (MQ=255)
                                     aTGCGCGTGATATTGACGAAGCGACGGACAGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGacac                                                                                                          <  7:233259/113‑1 (MQ=255)
                                      tGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGGCACACCGTTATCGGTCTCGTTTTAGATGAg                                                                               <  8:61428/139‑1 (MQ=255)
                                              atatTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGata                                                                        <  1:118032/138‑1 (MQ=255)
                                               tatTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGatat                                                                       <  2:245646/138‑1 (MQ=255)
                                                     cGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCAt                                                                <  6:77527/139‑1 (MQ=255)
                                                         gCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGatat                                                                       >  5:166442/1‑128 (MQ=255)
                                                         gCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGatat                                                                       <  6:166442/128‑1 (MQ=255)
                                                                  cGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTg                                                   <  4:333141/139‑1 (MQ=255)
                                                                    tgtAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGGCTCGTTTTAGATGAGGCAGATATGGTTGCTAAAGTTACGATTGtt                                                 >  1:300693/1‑139 (MQ=255)
                                                                    tgtAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATAGTGGCTTATCACCAAGGCCGACACACCGTTATCGGTCTCGTTTTATATGAGGCAGATATGGTTCATAAAGTAACGATTGt                                                  >  6:132343/1‑138 (MQ=255)
                                                                    tgtAATTGCCGGACCCGCT‑AGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTc                                                >  1:120666/1‑139 (MQ=255)
                                                                        aTTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCt                                              <  6:146746/138‑1 (MQ=255)
                                                                             ccgACCCGCTTAGCAGAGCCGCGTTATCACCAAGAAAGAACGCTTTATCGTGGCTTATCCCGAAGGCGGACACAGCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGGTCATAAAGTAACGATTGTTCCTCGGGGc                                        <  3:33489/137‑1 (MQ=255)
                                                                             cGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGc                                        <  6:402809/139‑1 (MQ=255)
                                                                             cGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGc                                        <  8:75585/139‑1 (MQ=255)
                                                                                   cGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCt                                  <  7:312911/139‑1 (MQ=255)
                                                                                     cTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCggg                                >  8:227986/1‑139 (MQ=255)
                                                                                           agagCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGgtta                          <  3:385386/139‑1 (MQ=255)
                                                                                             agCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGgttatg                        <  4:2197/139‑1 (MQ=255)
                                                                                             agCCGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGggtatg                        <  4:378134/139‑1 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGa                                                                                   <  7:286402/78‑1 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGa                                                                                   >  8:286402/1‑78 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGat                                                                         >  7:227349/1‑88 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGat                                                                         <  8:227349/88‑1 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTg                                                   >  7:179063/1‑110 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTg                                                   <  8:179063/110‑1 (MQ=255)
                                                                                               ccGCGTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGgttatgct                      >  3:136794/1‑139 (MQ=255)
                                                                                                  cgTTATCTCCAAGAAAGAACGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGgttatgctgtt                   <  2:120666/139‑1 (MQ=255)
                                                                                                                 agaaCGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGc                                        <  2:53429/103‑1 (MQ=255)
                                                                                                                 agaaCGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGc                                        >  1:53429/1‑103 (MQ=255)
                                                                                                                   aaCGCATTATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGGTTATGCTGTTATGCTGCCAAGagaaga  >  8:138619/1‑139 (MQ=255)
                                                                                                                         |                                                                                                                                    
GCGCTTGTAGCGGCTCGTCAAAACAAGAAAAAAATCGATGCGCGTGATATTGACGAAGCGACGGACCGTGTAATTGCCGGACCCGCTAAGAAGAGCCGCGTTATCTCCAAGAAAGAACGCAATATCGTGGCTTATCACGAAGGCGGACACACCGTTATCGGTCTCGTTTTAGATGAGGCAGATATGGTTCATAAAGTAACGATTGTTCCTCGGGGCCAGGCTGGCGGTTATGCTGTTATGCTGCCAAGAGAAGA  >  BS168_reference_genome/78115‑78368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑